We narrowed to 9,393 results for: Coli
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Plasmid#92315PurposeExpression of NTerm 6xHis tagged protein L (PL) mutant K16TAG for ncAA incoporation using the p70a promoter and UTR1DepositorInsertB1 domain of protein L (PL) mutant K16TAG
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialMutationPromoterp70b (p70a promoter with one mutation)Available sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR1-deGFP Y35TAG D193TAG-6xHis-T500
Plasmid#92227PurposeExpression of deGFP mutant Y35TAG D193TAG for ncAA incoporation using the p70a promoter and UTR1DepositorInsertdeGFP Y35TAG D193TAG
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialMutationResidues Y35 D193 were mutated into TAG (Amber) s…Promoterp70aAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR3-Nterm 6xHis mRFP K15TAG-T500
Plasmid#92319PurposeExpression of NTerm 6xHis tagged mRFP mutant K15TAG for ncAA incoporation using the p70a promoter and UTR3DepositorInsertOptimized NTerm 6xHis tagged monomeric RFP (mRFP)
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialMutationPromoterp70aAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR1-Nterm 6xHis mRFP K15TAG-T500
Plasmid#92318PurposeExpression of NTerm 6xHis tagged mRFP mutant K15TAG for ncAA incoporation using the p70a promoter and UTR1DepositorInsertOptimized NTerm 6xHis tagged monomeric RFP (mRFP)
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialMutationPromoterp70aAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70b-UTR1-NTerm 6xHis-zmADH-T500
Plasmid#92229PurposeExpression of zmADH using the p70b (weak version of the p70a promoter) promoter and UTR1DepositorInsertNTerm 6xHis Tagged zmADH
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialMutationPromoterp70b (p70a promoter with one mutation)Available sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR1-NTerm 6xHis-zmADH V86TAG-T500
Plasmid#92230PurposeExpression of zmADH mutant V86TAG for ncAA incoporation using the p70a promoter and UTR1DepositorInsertNTerm 6xHis Tagged zmADH
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialMutationResidue V86 mutated into TAG (Amber) stop codon f…Promoterp70aAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZS1[GalS-T]
Plasmid#60753PurposeContains PIq driving expression GalS-T, the Fucose inducible chimera with the TAN DBD.DepositorInsertsGalS-T
GalS-T
UseSynthetic BiologyTagsExpressionBacterialMutationChimeric LacI/GalR repressor with the TAN DBD and…PromoterAvailable sinceJuly 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pSm-Nuclease
Plasmid#132431PurposeExpresses Serratia marcescens nuclease in E. coli using T7 promoterDepositorInsertSerratia marcescens nuclease (codon optimized)
UseTagsNoneExpressionBacterialMutationPromoterT7Available sinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHyperTEV60
Plasmid#228496Purpose10xHis-SUMO (Smt3) tagged HyperTEV60 engineered protease (E. coli based protein expression)DepositorInsertTEV Protease
UseTags10xHis-SUMO(Smt3)ExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pOpen-DpnIR
Plasmid#165529PurposeUsed to digest methylated DNA; to be expressed in dam- E. coli strains (strains which do not methylate their genomic DNA)DepositorInsertDpnI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 5, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBOH-MHL
Plasmid#62304PurposeIt is a donor vector for generation of recombinant baculovirus by site-specific transposition in an E. coli host.DepositorTypeEmpty backboneUseBaculovirus expressionTags6xHis and TEV cleavage siteExpressionMutationPromoterpolyhedrinAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTRC-LIC
Plasmid#62343PurposeUsed for tac promoter regulated high-level expression of cloned, untagged genes in E. Coli.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromotertac promoter from pKK233-2Available sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
YopH-pNIC-Bsa4
Plasmid#92063PurposepNIC expression venctor encoding gene for YopHDepositorInsertYopH
UseTags6x His TagExpressionBacterialMutationCodon optimized E. coliPromoterAvailable sinceJan. 17, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
p28BIOH-LIC
Plasmid#62352PurposeE. coli expression vector with N-terminal AviTag and a C-terminal Hexa-HIS tagDepositorTypeEmpty backboneUseTags6xHis and AviTagExpressionBacterialMutationPromoterT7-lacOAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMalAnPL
Plasmid#67281PurposeExpresses MBP-Anacystis nidulans photolyase in E. coliDepositorInsertphotolyase Anacystis nidulans
UseTagsmaltose binding proteinExpressionBacterialMutationnone knownPromoterAvailable sinceJuly 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T1-NDFIP2
Plasmid#179330PurposeE. coli optimized expression of GST-fusion of human NDFIP2DepositorInsertNDFIP2 (NDFIP2 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceApril 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
Sc Rad27-pET24
Plasmid#239204PurposeOverexpress Sc Rad27 (FEN1) in E. coliDepositorInsertRad27 (RAD27 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET/His-Rab1a(Q70L)
Plasmid#232919PurposeE. coli ExpressionDepositorInsertRAB1A (RAB1A Human)
UseTagsHisExpressionBacterialMutationQ70LPromoterAvailable sinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAIP4_2RH3
Plasmid#234219PurposeHeterologous protein expression of protein virC2 in Escherichia coliDepositorInsert2RH3 (virC2 Agrobacterium tumefaciens)
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_5Y33
Plasmid#234204PurposeHeterologous protein expression of alginate lyase in Escherichia coliDepositorInsert5Y33
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_4REH
Plasmid#234195PurposeHeterologous protein expression of major latex-like protein in Escherichia coliDepositorInsert4REH
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_4ZHW
Plasmid#234158PurposeHeterologous protein expression of YfiB in Escherichia coliDepositorInsert4ZHW (yfiB Pseudomonas aeruginosa PAO1)
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_1XSZ
Plasmid#234151PurposeHeterologous protein expression of RalF in Escherichia coliDepositorInsert1XSZ (ralF Legionella pneumophila)
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_2D5J
Plasmid#234143PurposeHeterologous protein expression of unsaturated glucuronyl hydrolase in Escherichia coliDepositorInsert2D5J
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
MZB186
Plasmid#228489PurposeEmpty 10xHis-SUMO (Smt3) fused Avi-tag vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expressionDepositorTypeEmpty backboneUseTags10xHis-SUMO(Smt3)-AvitagExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
C84m
Plasmid#120995PurposeMoClo golden gate assembly CD part for sigma28/RpoF (RNA polymerase required for flagellar formation in E. coli). Please see Supplemental Documents for annotated Genbank file.DepositorInsertSigma 28
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pET28-iLOVf
Plasmid#63723PurposeE coli expression plasmid to produce Thrombin cleavable His tagged iLOV fusion proteinsDepositorTypeEmpty backboneUseTagsTEE, iLOV, 6xHis, thrombin cleavage siteExpressionBacterialMutationPromoterT7Available sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHN764
Plasmid#178819PurposeExpression of EndoH in E. coliDepositorInsertsynthetic gene encoding endo-β-N-acetylglucosaminidase H
UseTagsHIs8 and MBPExpressionBacterialMutationPromotertacAvailable sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a_SYK
Plasmid#224240PurposeExpression of human SYK Kinase in E. coliDepositorInsertsyk (SYK Human)
UseTags6xHis and 6xHis-TEVExpressionBacterialMutationPromoterT7 promotorAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pOPINN-GFP
Plasmid#53541PurposepTirex based GFP fusion vector for expression in mammalian, E. coli and insect cells using the baculovirus systemDepositorInsertGFP
UseTagsN-His-GFPExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-pOsmB::mCherry
Plasmid#53313PurposemCherry reporter plasmid for OsmB promoterDepositorInsertOsmB (osmB E.coli)
UseTagsmCherryExpressionBacterialMutationPromoterT7Available sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-pSoxS::mCherry
Plasmid#53311PurposemCherry reporter plasmid for SoxS promoterDepositorInsertSoxS promoter (soxS E.coli)
UseTagsmCherryExpressionBacterialMutationPromoterT7Available sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
Sc FLAG-rad24-pRSFDuet
Plasmid#240733PurposeOver-express FLAG-rad24 in E. coliDepositorInsertFLAG-rad24 (RAD24 Budding Yeast)
UseTags3X FLAGExpressionBacterialMutationPromoterAvailable sinceAug. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pN3
Plasmid#233520PurposeNb Vector Plasmid (under galactose induction), AmpR (E. coli) and HygroR (yeast), Cen6/ARSDepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterpGAL1Available sinceJuly 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pN4
Plasmid#233521PurposeNb Vector Plasmid (under galactose induction), AmpR (E. coli) and TRP1 (yeast), Cen6/ARSDepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterpGAL1-10Available sinceJuly 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET/His-GST-Rab1a(Q70L)
Plasmid#232911PurposeE. coli ExpressionDepositorInsertRAB1A (RAB1A Human)
UseTagsHis-GSTExpressionBacterialMutationQ70LPromoterAvailable sinceMarch 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAIP4_3W4Q
Plasmid#234236PurposeHeterologous protein expression of beta-lactamase in Escherichia coliDepositorInsert3W4Q
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits