We narrowed to 32,226 results for: Eng
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Plasmid#89901PurposeBacterial expression for bzCas13b and crRNA with both HEPN domains mutated. New spacers can be cloned by digesting with BsaI.DepositorInsertCas13b
ExpressionBacterialMutationR116A/H121A/R1177A/H1182APromoterLacAvailable SinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
plko-shmfn2-mfn2-Rescue
Plasmid#160655Purpose"Rescue mfn2 expression by adding the guide-resistant-mutant mfn2 sequence"DepositorAvailable SinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHRIG-PRAS40
Plasmid#53599Purposelentiviral expression of constitutively active PRAS40 and EGFPDepositorInsertPRAS40 (AKT1S1 Human)
UseLentiviralTagsIRES-EGFP and mycExpressionMammalianMutationconstitutively active (myristoylated version)PromoterCMVAvailable SinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV Gpat4-V5
Plasmid#175145PurposeLentiviral expression of mouse Gpat4-V5DepositorAvailable SinceJan. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-CaMKII-TurboID
Plasmid#227178PurposeCo-expression of FLAG-tagged TurboID and T2A-mCherry under the CaMKIIα short promoter by AAV vectorDepositorInsertTurboID
UseAAVTagsFLAG and T2A-mCherryExpressionMammalianPromoterCaMKIIa_shortAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMYs-MFN2-IRES-mitoRFP
Plasmid#121997PurposeExpression of MFN2 and mitoRFP in mammalial cellsDepositorInsertMFN2-IRES-mitoRFP (MFN2 Human)
UseRetroviralMutationsilent Q386Q Q387Q V388V Y389Y C390C E391E E392EAvailable SinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
human ETV6 gRNA-1
Plasmid#133353Purposehuman ETV6 gRNA-1 is a gRNA expression plasmid. Its 20-nt specific sequence targets the first ETV6 exon.DepositorAvailable SinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MBP-(CBX1)3-WT
Plasmid#180212PurposeBacterial expression of engineered CBX1 fusions with enhanced binding to H3K9meDepositorInsertCBX1 chromobox protein 1 x3 (CBX1 Human)
Tags6xhis-tag and MBPExpressionBacterialPromoterT7Available SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
BE3-hA3A-R128A
Plasmid#132944PurposeGene editing. See Gene/Insert section of the plasmid page for targeting sequence cloned into this plasmid.DepositorInsertBE3-hA3A(R128A)
UseCRISPR; Base editorExpressionMammalianMutationBE3-hA3A(R128A)Available SinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMTB2-cytoBirA-2A-mCherry_Ras
Plasmid#80066PurposeBeta-actin2 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialPromoterBeta-actin2Available SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX330-NLC-DHFR-SpCas9
Plasmid#124523PurposeExpresses SpCas9 fused to DHFR domains on both the N- and C- termini and an internal loop in mammalian cellsDepositorInsertNLC-DHFR-SpCas9
UseAAVTagsDestabilized domain of E.coli dihydrofolate reduc…ExpressionMammalianPromoterCbhAvailable SinceJuly 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMTB2-NLS-BirA-2A-mCherry_Ras
Plasmid#80067PurposeBeta-actin2 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialPromoterBeta-actin2Available SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
tdEos-Vimentin-7
Plasmid#57688PurposeLocalization: Intermediate Filaments, Excitation: 505 / 569, Emission: 516 / 581DepositorAvailable SinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEM-cytoBirA-2A-mCherry-SV40pA-FKF
Plasmid#79887PurposeDonor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertHA-tagged BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
ExpressionBacterialAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT-myl7-cytoBirA-2A-mCherry_Ras
Plasmid#80060PurposeMyl7 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialPromoterMyl7 promoterAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
TMED3-hLOV-TEVcs-GAL4bd-VP64-V5
Plasmid#170996PurposeHiLITR transcription factor with full-length TMED3 targeting sequence (ER/Golgi)DepositorInsertTMED3(FL)-NNES-hLOV-TEVcs(ENLYFQ/M)-GAL4bd-VP64-V5 (TMED3 Synthetic)
UseLentiviral and Synthetic BiologyTagsV5ExpressionMammalianPromoterEf1-alphaAvailable SinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3-hALDOA D34S
Plasmid#210810PurposeTo reduce the catalytic constant (kcat) for conversion of FBP to dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P)DepositorInsertALDOA (ALDOA Human)
TagsHAExpressionMammalianMutationresidues 34 of ALDOA mutated to serinePromoterCMVAvailable SinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
BE3-hA3A-Y130F
Plasmid#132945PurposeGene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.DepositorInsertBE3-hA3A(Y130F)
UseCRISPR; Base editorExpressionMammalianMutationBE3-hA3A(Y130F)Available SinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV V5-TbID-emerin
Plasmid#175099PurposeLentiviral expression of V5-TbID-tagged mouse EmdDepositorAvailable SinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMT-zic2a-cytoBirA-2A-mCherry_Ras
Plasmid#80064PurposeZic2a promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialPromoterZic2a enhancer driving c-fos promoterAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT-ubb-NLS-BirA-2a-mCherry
Plasmid#79886PurposeUbiquitin promoter driving HA-tagged biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
Tags3x HAExpressionBacterialPromoterUbiquitinAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT-MCS-NLS-BirA-2A-mCherry_Ras
Plasmid#80059PurposeMCS for cloning promoter to drive HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
Cas9_YTHDF2_sgRNA
Plasmid#186673PurposeYTHDF2 sgRNA plasmidDepositorInsertYTHDF2 KO sgRNA Plasmid (YTHDF2 Human)
ExpressionMammalianAvailable SinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GABARα1N-HA
Plasmid#128110PurposeExpress GABARα1 N terminal domainDepositorAvailable SinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEM-BirA-2A-H2B-Dendra2-SV40pA-FKF
Plasmid#119864PurposeDonor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), H2B-tagged Dendra2 fluorophore and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertBirA, 2A, H2B-tagged Dendra2 and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
TagsHA-tagged biotin ligase (BirA), Thosea asigna vir…ExpressionBacterialAvailable SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
Mouse GFP-PRMT7 LDIG(69-72)AAAA
Plasmid#65044PurposeExpresses Catalytically inactive GFP-PRMT7 LDIG(69-72)AAAA mutantDepositorInsertPRMT7 (Prmt7 Mouse)
UseBacterial propagationTagsGFPExpressionMammalianMutationL69A D70A I71A G72APromoterCMVAvailable SinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
tdEos-Keratin-17
Plasmid#57629PurposeNote- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 505 / 569, Emission: 516 / 581DepositorAvailable SinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTYB3-human PUM1-HD PUF531_Q867E-Q939E-C935S-Q1011E- C1007S-intein-CBD
Plasmid#73306PurposeExpresses mutant human PUM1-HDDepositorInserthuman PUM1 Pumilio homology domain (PUM1 Human)
TagsinteinExpressionBacterialMutationQ867E-Q939E-C935S-Q1011E-C1007SAvailable SinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHIVT9-NAT-3xFlag-SMARCAL1-ΔN
Plasmid#246936PurposeLentiviral vector that expresses Flag-tagged SMARCAL1 with a deletion of the RPA-binding domain located in the N-terminal region (ΔN)DepositorInsertSMARCAL1 (SMARCAL1 Human)
UseLentiviralTags3XFLAGExpressionMammalianPromoterEF-1-alpha promoterAvailable SinceDec. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHIVT9-NAT-3xFlag-SMARCAL1-R764Q
Plasmid#246937PurposeLentiviral vector that expresses Flag-tagged SMARCAL1 with a mutation in the ATP translocase domain (R764Q)DepositorInsertSMARCAL1 (SMARCAL1 Human)
UseLentiviralTags3XFLAGExpressionMammalianPromoterEF-1-alpha promoterAvailable SinceDec. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHIVT9-NAT-3xFlag-SMARCAL1
Plasmid#246935PurposeLentiviral vector that expresses wildtype Flag-tagged SMARCAL1DepositorInsertSMARCAL1 (SMARCAL1 Human)
UseLentiviralTags3XFLAGExpressionMammalianPromoterEF-1-alpha promoterAvailable SinceDec. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHNF4A-Enh_Gsta2-IRES2-H2BeGFP
Plasmid#247049PurposeThis plasmid was employed to generate the mouse model used in this study. It contains the human HNF4A gene enhancer linked to the Shh mini-promoter and the Gsta2-IRES2-H2BeGFP. Homologous armDepositorInsertGlutathione S-Transferase Alpha 2 (Gsta2 Mouse)
ExpressionMammalianAvailable SinceNov. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
MSCV-IRES-Thy1.1-Aoah
Plasmid#233141PurposeThy1.1 and Aoah overexpressionDepositorInsertAcyloxyacyl hydrolase (Aoah Mouse)
UseLentiviralAvailable SinceNov. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
PATagRFP-GRP78
Plasmid#238419PurposeExpresses GRP78 fused to the photoactivatable Tag-red fluorescent protein (PATagRFP-GRP78)DepositorAvailable SinceSept. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
GST-GRP78-N Terminus (pGEX4T1)
Plasmid#238422PurposeExpresses GRP78 N terminus fused to the GST TagDepositorAvailable SinceAug. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
GST-GRP78-C Terminus (pGEX4T1)
Plasmid#238423PurposeExpresses GRP78 C Terminus fused to the GST TagDepositorAvailable SinceAug. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-1 AMPK αKD(27-290aa)
Plasmid#227853PurposeTo express in E. coli AMPK αKD(27-290aa)DepositorAvailable SinceJuly 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.3 N-MYC TB182
Plasmid#227882PurposeUsed for transfection of cells for expression of C-MYC TB182DepositorAvailable SinceJuly 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.3 N-MYC PDHA1
Plasmid#227877PurposeUsed for transfection of cells for expression of C-MYC PDHA1DepositorAvailable SinceJuly 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.3 C-MYC pdzd-8(536A)
Plasmid#227885PurposeUsed for transfection of cells for expression of C-MYC pdzd-8(536A)DepositorAvailable SinceJuly 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.3 N-MYC pdzd-8
Plasmid#227844PurposeUsed for transfection of cells for expression of C-MYC pdzd-8DepositorAvailable SinceJune 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
sg_Snrnp48_i1-ipUSEPR-TR657
Plasmid#228925PurposeKnockdown of Snrnp48 in mammalian cellsDepositorInsertsg_Snrnp48_i1 (Snrnp48 Mouse)
UseLentiviralAvailable SinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
sg_Tada3_i3-ipUSEPR-TR657
Plasmid#228915PurposeKnockdown of Tada3 in mammalian cellsDepositorInsertsg_Tada3_i3 (Tada3 Mouse)
UseLentiviralAvailable SinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
sg_Tada3_i5-ipUSEPR-TR657
Plasmid#228916PurposeKnockdown of Tada3 in mammalian cellsDepositorInsertsg_Tada3_i3 (Tada3 Mouse)
UseLentiviralAvailable SinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
sg_Atm_i3-ipUSEPR-TR657
Plasmid#228917PurposeKnockdown of Atm in mammalian cellsDepositorInsertsg_Atm_i3 (Atm Mouse)
UseLentiviralAvailable SinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
sg_Atm_i5-ipUSEPR-TR657
Plasmid#228918PurposeKnockdown of Atm in mammalian cellsDepositorInsertsg_Atm_i5 (Atm Mouse)
UseLentiviralAvailable SinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
sg_Snrnp48_i4-ipUSEPR-TR657
Plasmid#228926PurposeKnockdown of Snrnp48 in mammalian cellDepositorInsertsg_Snrnp48_i4 (Snrnp48 Mouse)
UseLentiviralAvailable SinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-NPR4 (SA binding domain)
Plasmid#226885PurposeExpresses the Salicylic acid binding core (SBC) of NPR4 in E.coli, in an insoluble formDepositorInsertThe Salicylic acid (SA) binding domain of NPR4 (NPR4 Mustard Weed)
TagsHis tagExpressionBacterialMutationNPR4 SA-binding core (residues 373-516), with a s…PromoterT7Available SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
B2M-SDMutation-gRNA
Plasmid#215547PurposegRNA targeting B2M to introduce splice donor mutation on the first intron of the locusDepositorInsertB2M (B2M Human)
UseCRISPRAvailable SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFlare9A-Clip2E9 WT
Plasmid#209574Purposeminigene construct for Clip2 E9 alternative splicingDepositorAvailable SinceMarch 7, 2024AvailabilityAcademic Institutions and Nonprofits only