We narrowed to 19,524 results for: ACE

1. pQLinkHD_EBFP2

   Plasmid

   #118853

   Purpose

   fluorescent protein expression with an N-terminal His-tag

   Depositor

   Marc Birtwistle

   Article

   Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

   Insert

   EBFP2
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   EBFP1.2-I128V/V150I/ D155V/V224R

   Promoter

   Available since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQE-WT-N4-R5-RNRV

   Plasmid

   #63630

   Purpose

   N4-R5-RNRV RNAP mutant

   Depositor

   Andrew Ellington

   Article

   Meyer et al ACS Synth Biol. 2014 Oct 7.

   Insert

   N4-R5-RNRV RNAP
   

   Use

   Tags

   Expression

   Mutation

   L747I, N748D, L749C, M750V, F751I, Q754T, F755R, …

   Promoter

   T5/Lac

   Available since

   April 15, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pHelper_O

   Plasmid

   #173783

   Purpose

   The 186 helper plasmid necessary for genomic integration of the pIT5_KO plasmid.

   Depositor

   Keith Shearwin

   Article

   Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

   Insert

   186 Integrase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   E59G - please see depositor comments

   Promoter

   Available since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHelper_W

   Plasmid

   #173784

   Purpose

   The Wphi helper plasmid necessary for genomic integration of the pIT5_KW plasmid.

   Depositor

   Keith Shearwin

   Article

   Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

   Insert

   Wphi Integrase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHelper_D

   Plasmid

   #173781

   Purpose

   The D145 helper plasmid necessary for genomic integration of the pIT5_KD plasmid.

   Depositor

   Keith Shearwin

   Article

   Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

   Insert

   D145 Integrase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TDGS50

   Plasmid

   #183189

   Purpose

   TEV protease expressed under J23106 promoter; DivIVAsfGFP-TEV protease recognition site-M0050 expressed under J23116 promoter

   Depositor

   Hsiao-Chun Huang

   Article

   Hong et al ACS Synth Biol. 2021 Aug 20;10(8):1830-1

   Inserts

   DivIVA-GFP(tevR M0050)
   TEV-S219V
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pICH45152

   Plasmid

   #50263

   Depositor

   Sylvestre Marillonnet, Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   promoter, cab1 (AT1g29930, A. thaliana)
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Promoter

   Available since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUCSh3-mRFP

   Plasmid

   #170983

   Purpose

   Encodes sgRNA scaffold with mRFP in place of target and Tetracycline resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.

   Depositor

   Christopher Reisch

   Article

   Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

   Insert

   mRFP
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   July 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMM705

   Plasmid

   #68892

   Purpose

   IPTG-inducible CRISPRi vector targeting BT1754, pNBU2 backbone, AmpR

   Depositor

   Timothy Lu, Christopher Voigt

   Article

   Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

   Inserts

   dCas9
   LacI
   sgRNA
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. Golgi-DKAR

    Plasmid

    #49790

    Purpose

    FRET-based, Golgi-targeted kinase activity reporter for PKD

    Depositor

    Alexandra Newton

    Article

    Kunkel et al J Biol Chem. 2010 Jul 23;285(30):22748-5

    Insert

    D kinase activity reporter
    

    Use

    Tags

    CFP, YFP, and eNOS(1-33)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pICSL30003

    Plasmid

    #50304

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    CDS, no stop codon, mCherry variant of RFP (Discosoma sp.)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZA2[PLac:mCherry,PTAN:GFP]

    Plasmid

    #60780

    Purpose

    Contains PLac driving expression of mCherry and PTAN driving expression of GFP.

    Depositor

    Matthew Bennett

    Article

    Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

    Inserts

    mCherry
    yemGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBbB13k-GFP

    Plasmid

    #126122

    Purpose

    Constitutive expression vector for Escherichia coli. BglBrick compatible.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

    Insert

    GFPuv
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23102

    Available since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pICSL30004

    Plasmid

    #50302

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    CDS no stop codon, YFP variant of GFP (Aequorea victoria)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFTK056

    Plasmid

    #171328

    Purpose

    Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

    Depositor

    Arnold Driessen

    Article

    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

    Insert

    SpCas9-NLS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    n/a

    Promoter

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBP-TL4

    Plasmid

    #72988

    Purpose

    RBS_H5

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    H5 RBS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pBP-TL8

    Plasmid

    #72992

    Purpose

    RBS_L1

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    L1 RBS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pBP-L1U1H09

    Plasmid

    #73008

    Purpose

    L1U1H09 terminator

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    L1U1H09 terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pICH71421

    Plasmid

    #51834

    Purpose

    Depositor

    Sylvestre Marillonnet, Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    plant 3'UTRpolyadenylation signal/terminator, Tom H4
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available since

    March 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFTK088

    Plasmid

    #171360

    Purpose

    AMA1 sequence as Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

    Depositor

    Arnold Driessen

    Article

    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

    Insert

    AMA1 sequence (short), entry vector providing fungal replication
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    n/a

    Promoter

    Available since

    Jan. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge