We narrowed to 17,819 results for: Mut

1. dRT-pMDLg/pRRE

   Plasmid

   #60488

   Purpose

   gag/pol packaging plasmid for production of lentiviral vectors with mutated reverse transcriptase (NRTLV, non-reverse transcribable lentiviral vectors)

   Depositor

   Boris Fehse

   Article

   Mock et al Sci Rep. 2014 Sep 18;4:6409. doi: 10.103

   Insert

   HIV-1 gag, HIV-1 pol with inactive reverse transcriptase
   

   Use

   Tags

   Expression

   Mutation

   changed Asp at position 249 + 250 to Val

   Promoter

   Available Since

   Oct. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pORTMAGE312B

   Plasmid

   #128969

   Purpose

   Broad-host-range KanR plasmid expressing PapRecT and EcMutL_E32K with Toluic acid induction

   Depositor

   George Church

   Article

   Wannier et al Proc Natl Acad Sci U S A. 2020 Jun 16;11

   Inserts

   PapRecT
   mutL E32K
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   E32K

   Promoter

   xylS-Pm

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAT253

   Plasmid

   #40943

   Depositor

   Angela Taddei

   Article

   Dubarry et al Genes Dev. 2011 Jul 1;25(13):1365-70.

   Insert

   GFP-LacI**
   

   Use

   Tags

   GFP(S65T, V163A) and SV40 NLS

   Expression

   Yeast

   Mutation

   LacI**/LacI2G(4 mutations) (see comment below); l…

   Promoter

   His3 promoter fragment

   Available Since

   Feb. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBAD-tdTEVDB_C-15A/G4C

   Plasmid

   #177205

   Purpose

   Permuted td intron with evolved initiation sequence for the expression of circular mRNA

   Depositor

   Stephen Fried

   Article

   Lee et al ACS Cent Sci. 2021 Oct 27;7(10):1736-175

   Insert

   Permuted GFP with evolved initiation sequence for circular mRNA
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Permuted sfGFP after residue 52 & Changed Cyt…

   Promoter

   araBAD

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-FLEX-SaCas9-U6-sgSlc18a2

   Plasmid

   #124849

   Purpose

   Mutagenesis of Slc18a2

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Slc18a2 (Slc18a2 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-FLEXfrt-SaCas9-U6-sgSlc18a2

   Plasmid

   #124862

   Purpose

   Mutagenesis of Slc18a2

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Slc18a2 (Slc18a2 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. cpLID-micro

   Plasmid

   #168995

   Purpose

   circularly permuted LOV2 based optical dimerization system (ssrA-cpLOV2 and sspB)

   Depositor

   Yubin Zhou

   Article

   He et al Nat Chem Biol. 2021 Aug;17(8):915-923. d

   Insert

   mCh-sspB-T2A-cpLID-CAAX
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-FLEX-SaCas9-U6-sgSlc17a8

   Plasmid

   #124848

   Purpose

   Mutagenesis of Slc17a8

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Slc17a8 (Slc17a8 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCS2TAL3-DD

   Plasmid

   #37275

   Purpose

   TALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-terminal tal protein segments increases mutation induction; Fok I DD (improved version=pCS2TAL3-DDD)

   Depositor

   David Grunwald

   Article

   Dahlem et al PLoS Genet. 2012 Aug;8(8):e1002861. Epub

   Type

   Empty backbone

   Use

   Tags

   Flag, Fok I DD, Tal-C, and Tal-N

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAT222

    Plasmid

    #40942

    Depositor

    Angela Taddei

    Article

    Dubarry et al Genes Dev. 2011 Jul 1;25(13):1365-70.

    Insert

    GFP-LacI**
    

    Use

    Tags

    GFP(S65T, V163A) and SV40 NLS

    Expression

    Yeast

    Mutation

    LacI**/LacI2G(4 mutations) (see comment below); l…

    Promoter

    His3 promoter fragment

    Available Since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. E3020X

    Plasmid

    #21366

    Depositor

    Gregory Germino

    Article

    Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

    Insert

    autosomal dominant polycystic kidney disease type I (PKD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Changed E3020 to stop codon

    Promoter

    Available Since

    Feb. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pSALECTNK-TEM1(S70A,D179G)/csTEM1

    Plasmid

    #81163

    Purpose

    pSALECT plasmid with TEM-1 beta-lactamase fused to TEM-1

    Depositor

    Timothy Whitehead

    Article

    Klesmith et al Proc Natl Acad Sci U S A. 2017 Feb 14. p

    Insert

    TEM-1 beta-lactamase
    

    Use

    Tags

    Codon swapped TEM-1 from H26 to W290 and His6x

    Expression

    Bacterial

    Mutation

    Deleted the sec transport tag. Plasmid encodes H2…

    Promoter

    lac promoter

    Available Since

    Feb. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. GluR2_adRNA(1,20,6)-ADAR2(E488Q)

    Plasmid

    #124621

    Purpose

    Expresses adRNA(1,20,6) targeting the RAB7A transcript along with the ADAR2(E488Q)

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

    Insert

    ADAR2(E488Q)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    E488Q hyperactive mutant

    Promoter

    CMV

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAGH42

    Plasmid

    #107249

    Purpose

    Vipp1-mGFPmut3 (or vipp1-gfp) construct with spectinomycin resistance cassette downstream used to replace the native copy of Vipp1 in Synechocystis (sll0617).

    Depositor

    Erin O'Shea

    Article

    Gutu et al Mol Microbiol. 2018 Jan 22. doi: 10.1111

    Insert

    vipp1-gfp
    

    Use

    Suicide vector for destined for double homologous…

    Tags

    mGFPmut3

    Expression

    Bacterial

    Mutation

    Promoter

    native promoter of sll0617

    Available Since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET21b-Caspase-3(D3A,V266E)

    Plasmid

    #90091

    Purpose

    Expresses caspase D3A and V266E mutant in bacterial cells

    Depositor

    Clay Clark

    Article

    Pop et al Biochemistry. 2003 Oct 28;42(42):12311-2

    Insert

    Caspase 3 (CASP3 Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    D175A D28A D9A V266E

    Promoter

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-SI-121

    Plasmid

    #131126

    Purpose

    A-to-G base editing reporter

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    mutant EGFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available Since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-FLEXfrt-SaCas9-U6-sgSlc17a8

    Plasmid

    #124861

    Purpose

    Mutagenesis of Slc17a8

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc17a8 (Slc17a8 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAG306-GPD-VMA1 chr I

    Plasmid

    #41896

    Purpose

    Yeast expression of VMA1.

    Depositor

    Dan Gottschling

    Article

    Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

    Insert

    VMA1 (VMA1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Contains silent mutation at 342A-G and L87S mutat…

    Promoter

    GPD

    Available Since

    April 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFAB808

    Plasmid

    #47853

    Purpose

    BIOFAB reporter plasmid for measuring rrnB T1 termination efficiency

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    rrnB T1 terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLTetO8

    Available Since

    Dec. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pC-KIT5

    Plasmid

    #118987

    Purpose

    Mutational dissection of the G rich region of the c-kit promoter

    Depositor

    Bjorn Hogberg

    Article

    Ducani et al J Am Chem Soc. 2019 Jul 3;141(26):10205-

    Insert

    c-kit proto-oncogene {promoter} (KIT Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mutK2-mutSP-K1

    Promoter

    Available Since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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