We narrowed to 3,225 results for: sam

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pLD-puro-Cn-VA-NHEJ1-WT

Plasmid

#141339

Purpose

Lentiviral vector for expression of human NHEJ1-WT in mammalian cells

Depositor

Mohan Babu

Article

Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

Insert

NHEJ1 (NHEJ1 Human)

Use

Lentiviral

Tags

VA tag (3XFLAG-2XTEV-6XHis-2XStrep-Beacon)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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AIP(WT)-mChF-giantin

Plasmid

#61523

Purpose

Expression of human golbin B1 and FK506 binding protein 1A (FKBP1A) tagged with mCherry and AIP

Depositor

Takanari Inoue

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Inserts

FK506 binding protein 1A (FKBP1A Human)
golgin B1 (GOLGB1 Human)

Tags

AIP and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
pYX-C-mScarlet-Zeo-TSC22D2

Plasmid

#222670

Purpose

endogenously tag TSC22D2 with C-terminal mScarlet

Depositor

Jason Moffat

Article

Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

Insert

TSC22D2 (TSC22D2 Human)

Tags

mScarlet

Expression

Bacterial

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR 223 VPS4A

Plasmid

#169319

Purpose

Gateway entry vector for human VPS4A ORF

Depositor

Francisca Vazquez

Article

Neggers et al Cell Rep. 2020 Dec 15;33(11):108493. doi

Insert

VPS4A (VPS4A Human)

Use

Gateway Cloning

Promoter

NA

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-PPO-Venus

Plasmid

#139504

Purpose

AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the CAG promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.

Depositor

Michael Bruchas

Article

Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

Has Service

AAV5 and AAV9

Insert

parapinopsin

Use

AAV

Tags

Venus tag following 3x Ala linker

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR 223 VPS4B

Plasmid

#169320

Purpose

Gateway entry vector for human VPS4B ORF

Depositor

Francisca Vazquez

Article

Neggers et al Cell Rep. 2020 Dec 15;33(11):108493. doi

Insert

VPS4B (VPS4B Human)

Use

Gateway Cloning

Promoter

NA

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS/XBB.1.16-RBD

Plasmid

#213070

Purpose

Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.XBB.1.16 variant

Depositor

Priyamvada Acharya

Article

Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

Insert

pCAGGS/XBB.1.16-RBD (S )

Tags

6 His Tag

Expression

Mammalian

Mutation

G339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pαH-S-GSAS-2P-Omicron-BA.5

Plasmid

#213828

Purpose

Mammalian cell expression of SARS-CoV-2 Spike protein of OMICRON.2P.BA5 variant

Depositor

Priyamvada Acharya

Article

Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

Insert

pαH-S-GSAS/Omicron-2P-BA5 (S )

Tags

HRV 3C cleavage site (before tags) 8X His tag 2X…

Expression

Mammalian

Mutation

G339D,R346T,S371F,S373P,S375F,T376A,D405N,R408S,K…

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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BCL6-GFP G55A in Artichoke

Plasmid

#169935

Purpose

Overexpression of BCL6-GFP G55A mutant fusion

Depositor

Benjamin Ebert

Article

Slabicki et al Nature. 2020 Dec;588(7836):164-168. doi:

Insert

BCL6 G55A (BCL6 Human)

Use

Lentiviral

Tags

GFP

Mutation

160R>C

Available Since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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LMP AMPK alpha2 hp1

Plasmid

#89490

Purpose

shRNA against AMPK alpha2 in mouse/human to knock down protein expression

Depositor

Russell Jones

Article

Faubert et al Cell Metab. 2013 Jan 8;17(1):113-24. doi

Insert

hairpin against mouse/human AMPKalpha2 (PRKAA2, Prkaa2 )

Use

Mouse Targeting and Retroviral

Expression

Mammalian

Promoter

LMP

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only
pYX-N-Puro-mNeon-WNK3

Plasmid

#222674

Purpose

endogenously tag WNK3 with N-terminal mNeon

Depositor

Jason Moffat

Article

Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

Insert

WNK3 (WNK3 Human)

Tags

mNeon

Expression

Bacterial

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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LeGO-iG-CSF2RB(A455D)

Plasmid

#125751

Purpose

Lentiviral Expression Vektor with CSF2RB-A455D mutant cDNA and IRES eGFP

Depositor

Dirk Heckl

Article

Labuhn et al Cancer Cell. 2019 Aug 12;36(2):123-138.e

Insert

CSF2RB(A455D)

Use

Lentiviral

Mutation

A455D, codon optimized

Promoter

SFFV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 NSP12_nostop

Plasmid

#149314

Purpose

Gateway-compatible Entry vector, with insert of NSP12 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP12 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-T7-NLS(SV40)-(Spyo_Cas9)-6xHis-NLS(SV40)

Plasmid

#185706

Purpose

CMV and T7 promoter expression plasmid for human codon usage anCas(Spyo_Cas9) with an N-terminal NLS and C-terminal NLS

Depositor

Raul Perez-Jimenez

Article

Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

Insert

Cas9

Tags

6xHis-NLS and NLS

Expression

Mammalian

Mutation

optimized for human codon usage

Promoter

CMV and T7

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS/XBB.1.5-RBD

Plasmid

#213038

Purpose

Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.XBB.1.5 variant

Depositor

Priyamvada Acharya

Article

Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

Insert

pCAGGS/XBB.1.5-RBD (S )

Tags

6 His Tag

Expression

Mammalian

Mutation

G339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pMMK ENTR 1 CMV mAG beta-catenin

Plasmid

#206258

Purpose

ENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mAG Ctnnb1 under the control of CMV promoter.

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

mAG Ctnnb1 (Ctnnb1 Mouse)

Use

Multimate/gateway entr 1

Tags

monomeric Azami Green

Expression

Mammalian

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pEMS1503

Plasmid

#29299

Purpose

Homologous recombination plasmid for gene targeting at the mouse Hprt locus containing Ple32 (CLDN5 MiniPromoter) driving a lacZ reporter.

Depositor

Elizabeth Simpson

Article

de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

Insert

Ple32 (CLDN5 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Available Since

June 10, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pPICZc-Ub-R-HsACTB*-thymosinB-8His

Plasmid

#111149

Purpose

Expresses arginylated human beta-actin fused with thymosin beta and a His tag.

Depositor

Mohan Balasubramanian

Article

Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827

Insert

ACTB (ACTB Human)

Use

Pichia pastoris integration plasmid

Tags

Thymosin beta and a His-tag

Expression

Yeast

Mutation

N-terminal arginylation

Promoter

AOX1

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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BCL6-GFP Y58A in Artichoke

Plasmid

#169936

Purpose

Overexpression of BCL6-GFP Y58A mutant fusion

Depositor

Benjamin Ebert

Article

Slabicki et al Nature. 2020 Dec;588(7836):164-168. doi:

Insert

BCL6 Y58A (BCL6 Human)

Use

Lentiviral

Tags

GFP

Mutation

160R>C

Available Since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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BCL6-GFP R28A in Artichoke

Plasmid

#169933

Purpose

Overexpression of BCL6-GFP R28A mutant fusion

Depositor

Benjamin Ebert

Article

Slabicki et al Nature. 2020 Dec;588(7836):164-168. doi:

Insert

BCL6 R28A (BCL6 Human)

Use

Lentiviral

Tags

GFP

Mutation

160R>C

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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