We narrowed to 3,095 results for: sam

1. pCMV-T7-NLS(SV40)-anCas(PCA)-6xHis-NLS(SV40)

   Plasmid

   #185704

   Purpose

   CMV and T7 promoter expression plasmid for human codon usage anCas(PCA) with an N-terminal NLS and C-terminal NLS

   Depositor

   Raul Perez-Jimenez

   Article

   Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

   Insert

   anCas(PCA)
   

   Tags

   6xHis-NLS and NLS

   Expression

   Mammalian

   Mutation

   optimized for human codon usage

   Promoter

   CMV and T7

   Available Since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-T7-NLS(SV40)-anCas(BCA)-6xHis-NLS(SV40)

   Plasmid

   #185702

   Purpose

   CMV and T7 promoter expression plasmid for human codon usage anCas(BCA) with an N-terminal NLS and C-terminal NLS

   Depositor

   Raul Perez-Jimenez

   Article

   Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

   Insert

   anCas(BCA)
   

   Tags

   6xHis-NLS and NLS

   Expression

   Mammalian

   Mutation

   optimized for human codon usage

   Promoter

   CMV and T7

   Available Since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGK-Ldb3-mKate2

   Plasmid

   #128766

   Purpose

   Fluorescent mKate reporter for Ldb3

   Depositor

   Deepak Srivastava

   Article

   Stone et al Cell Stem Cell. 2019 Jul 3;25(1):87-102.

   Insert

   Ldb3 promoter (Ldb3 Mouse)
   

   Expression

   Mammalian

   Promoter

   Ldb3

   Available Since

   Nov. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. MBP-rPKL S12TAG

   Plasmid

   #107155

   Purpose

   MBP-rPKL construct for production and purification of rat PKL pS12

   Depositor

   Jesse Rinehart

   Article

   Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

   Insert

   MBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)
   

   Tags

   C-term 6-his and N-term MBP

   Expression

   Bacterial

   Mutation

   Serine 12 to TAG for phosphoserine insertion

   Promoter

   PBAD

   Available Since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. MBP-rPKL S113TAG

   Plasmid

   #107157

   Purpose

   MBP-rPKL S113TAG for production and purification of rat PKL pS113

   Depositor

   Jesse Rinehart

   Article

   Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

   Insert

   MBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)
   

   Tags

   C-term 6-his and N-term MBP

   Expression

   Bacterial

   Mutation

   Serine 113 changed to TAG for phosphoserine inser…

   Promoter

   PBAD

   Available Since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAav-TP53-PQS1-3xFLAG

   Plasmid

   #84884

   Purpose

   rAAV-based template for genome engineering of the p53 C-terminus containing PQS1 and 3xFLAG tags and a selection cassette

   Depositor

   Sven Eyckerman

   Article

   Vandemoortele et al Sci Rep. 2016 Jun 6;6:27220. doi: 10.103

   Inserts

   TP53 genomic region HR1 (TP53 Human)
   TP53 genomic region HR2
   

   Use

   AAV

   Tags

   PQS1 3xFLAG

   Promoter

   no and no

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEMS1499

   Plasmid

   #29247

   Purpose

   Pleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).

   Depositor

   Elizabeth Simpson

   Article

   de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

   Insert

   Ple28 (CCL27 Human)
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Tags

   Intron-LacZ-NLS

   Available Since

   Nov. 7, 2011

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pEMS1498

   Plasmid

   #29246

   Purpose

   Pleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).

   Depositor

   Elizabeth Simpson

   Article

   de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

   Insert

   Ple27 (CCL27 Human)
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Tags

   Intron-LacZ-NLS

   Available Since

   Nov. 7, 2011

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pEMS1493

   Plasmid

   #29241

   Purpose

   Pleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).

   Depositor

   Elizabeth Simpson

   Article

   de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

   Insert

   Ple22 (CCKBR Human)
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Tags

   Intron-LacZ-NLS

   Available Since

   Nov. 7, 2011

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pEMS1568

    Plasmid

    #29265

    Purpose

    Pleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).

    Depositor

    Elizabeth Simpson

    Article

    de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

    Insert

    Ple97 (GPX3 Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Intron-LacZ-NLS

    Available Since

    Oct. 21, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pEMS1500

    Plasmid

    #29248

    Purpose

    Pleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).

    Depositor

    Elizabeth Simpson

    Article

    de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

    Insert

    Ple29 (CCL27 Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Intron-LacZ-NLS

    Available Since

    Oct. 20, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pEMS1215

    Plasmid

    #29109

    Purpose

    Expression of the eGFP reporter gene was not detected in the brain and eye.

    Depositor

    Elizabeth Simpson

    Article

    de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

    Insert

    Ple97 (GPX3 Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Available Since

    Oct. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. RED

    Plasmid

    #139835

    Purpose

    Expresses RNase H1-EGFP- dCas9 fusion protein in human cells

    Depositor

    Karim Mekhail

    Article

    Abraham et al Nature. 2020 Sep;585(7824):298-302. doi:

    Insert

    RNase H1-EGFP-dCas9 (RNASEH1 RNase H1 (H. sapiens), dCas9 (S. pyogenes))
    

    Expression

    Mammalian

    Mutation

    dCas9 protein contains two mutations: D10A and H8…

    Promoter

    tet-inducible CMV

    Available Since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. dRED

    Plasmid

    #139836

    Purpose

    Expresses dRNase H1-EGFP-dCas9 fusion protein in human cells

    Depositor

    Karim Mekhail

    Article

    Abraham et al Nature. 2020 Sep;585(7824):298-302. doi:

    Insert

    dRNase H1-EGFP-dCas9 (RNASEH1 dRNase H1 (H. sapiens), dCas9 (S. pyogenes))
    

    Expression

    Mammalian

    Mutation

    RNase H1 protein contains D210N mutation. dCas9 …

    Promoter

    tet-inducible CMV

    Available Since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pZ P (cHS4)X4 TetON-3XFLAG-tdT CAGG-m2rtTA v2

    Plasmid

    #112669

    Purpose

    Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows inducible expression and contains cHS4 insulators.

    Depositor

    Catherine Verfaillie

    Article

    Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-

    Insert

    tdT
    

    Use

    AAV; Donor plasmid for recombinase-mediated casse…

    Expression

    Mammalian

    Available Since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. P3-Lenti-dCas9-Tet1-GFP

    Plasmid

    #190729

    Purpose

    Lentiviral construct to express spdCas9-Tet1-GFP.

    Depositor

    Maja Jagodic

    Article

    Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:

    Insert

    dCas9-tet1
    

    Use

    CRISPR

    Tags

    SV40 NLS and T2A-EGFP

    Expression

    Mammalian

    Mutation

    Changed Pro434 codon from CCG to CCC and Gly468 f…

    Promoter

    UbC

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCag SE (Self-excising) FlpO-2A-Cre EV

    Plasmid

    #130986

    Purpose

    episomal expression of FlpO and Cre recombinases, self excising

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    FlpO-2A-Cre
    

    Use

    Cre/Lox and Unspecified; Episomal expression vect…

    Expression

    Mammalian

    Mutation

    protamine intron added to FlpO to prevent bacteri…

    Promoter

    CMV/Chick β-actin (CAG)

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. LeGO-iG-CSF2RB

    Plasmid

    #125750

    Purpose

    Lentiviral Expression Vektor with CSF2RB wildtype cDNA and IRES eGFP

    Depositor

    Dirk Heckl

    Article

    Labuhn et al Cancer Cell. 2019 Aug 12;36(2):123-138.e

    Insert

    CSF2RB(wt) (CSF2RB Human, 2694)
    

    Use

    Lentiviral

    Mutation

    codon optimized

    Promoter

    SFFV

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCG_SARS-CoV2/bat-CoV RaTG13 E C-V5-tag

    Plasmid

    #179977

    Purpose

    Mammalian expression vector for SARS-CoV-2 E or bat RaTG13 E, V5-tagged (SARS-CoV-2 E and RaTG13 E have the same amino acid sequence and codon optimized sequence).

    Depositor

    Konstantin Sparrer

    Article

    Hayn et al Cell Rep. 2021 May 18;35(7):109126.

    Insert

    SARS-CoV-2 E (E Synthetic)
    

    Expression

    Mammalian

    Mutation

    human codon-optimized

    Available Since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. MADR pDonor TagBFP2-3XFlag (cyto) WPRE

    Plasmid

    #129421

    Purpose

    MADR donor plasmid for FlpO+Cre-mediated insertion of a TagBFP2-3XFlag cytoplasmic reporter protein

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    TagBFP2-3Flag
    

    Use

    Mosaic analysis for dual recombinase-mediated cas…

    Tags

    3X Flag epitope tag

    Promoter

    none (3X polyA to mitigate episomal expression)

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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