We narrowed to 9,586 results for: Coli
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Plasmid#101149PurposeFluorescent protein for superresolution imagingDepositorInsertE. coli codon optimized mMaple2
ExpressionBacterialPromoterpBADAvailable SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTH1-SaSrtA-Q
Plasmid#64980PurposeExpresses the E108Q mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
TagsHis6 tag, Mxe GyrA intein, and chitin-binding pro…ExpressionBacterialMutationdeleted amino acids 1-59, E108QAvailable SinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
CHIKV-nsP3-c001
Plasmid#173086PurposeExpression of recombinant fusion protein in E. coliDepositorInsertN/A
TagsmCeruleanExpressionBacterialMutationA1334-T1493, codon optimizedAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMZ2007
Plasmid#204399PurposeA 3OC6-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.DepositorInsertsquorum sensing regulator LuxR
GFP
UseSynthetic BiologyPromoterPftsH and Synthetic promoter PLux0002Available SinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
p70rg
Plasmid#17827DepositorInsertE. coli optimized red flourescent protein (RFPec), GFPuv
UseSynthetic BiologyExpressionBacterialAvailable SinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
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pIA1421
Plasmid#172407PurposeExpression of SARS-COV-2 Nsp12 K50A in E. coliDepositorAvailable SinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSecEffector
Plasmid#172891PurposeContains TEVp display cassette, atc inducible (in any E. coli strain) BxbI and arabinose inducible TP901DepositorInsertsBxb1
TP901
TEV Display cassette
ProD promoter between anti aligned Bxb1 sites
ExpressionBacterialPromoterProD, nTETO, and pBAD /AraCAvailable SinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-nGFPMNase
Plasmid#187826Purposeexpress nGFPMNase protein in E coliDepositorInsertnGFPMNASE
Tagshis tagExpressionBacterialPromoterT7 PromotorAvailable SinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTYF-1xSYN-tTA
Plasmid#19980DepositorInsertSYN-tTA
ExpressionMammalianAvailable SinceAug. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBXPC3H-Syb37
Plasmid#123627PurposeExpresses Syb37 in e. coli cells, with Arabinose induction and Ampicillin selectionDepositorInsertSyb37
Tags3C-His and PelBExpressionBacterialAvailable SinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJT103
Plasmid#25087DepositorInsertPompC-lacZ
UseSynthetic BiologyExpressionBacterialAvailable SinceJune 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT/pLux LasI
Plasmid#193628PurposeInducible expression of LasI by the transcription factor complex LuxR-3OC6 homoserine lactone (HSL). LasI is codon optimized for E. coli.DepositorInsertLasI
Tags6xHisExpressionBacterialPromoterLux promoterAvailable SinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pFLIP38
Plasmid#65616PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-edCit-attR1 and attR2-edCer-cMycExpressionBacterialPromoterT7 promoterAvailable SinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-PcPT-ximD-ximE
Plasmid#172654PurposeProduction of decursinol in E. coliDepositorInsertPcPT, XimD and XimE
UseSynthetic BiologyAvailable SinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD-pnGFP-Ultra
Plasmid#157923PurposePeroxynitrite sensor pnGFP-Ultra in E. coli (use with pEvol-BoF)DepositorInsertpnGFP-Ultra, a high performance peroxynitrite sensor
ExpressionBacterialPromoteraraBADAvailable SinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFLIP43
Plasmid#65618PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-edAFPt9-attR1 and attR2-edCer-cMycExpressionBacterialPromoterT7 promoterAvailable SinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pQE80_T5_P3-ehaA_LacI
Plasmid#186315PurposePlasmid for inducible E coli expression of the P3 helixCAM.DepositorInsertP3-ehaA
ExpressionBacterialAvailable SinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQE80_T5_AP4-ehaA_LacI
Plasmid#186316PurposePlasmid for inducible E coli expression of the AP4 helixCAM.DepositorInsertAP4-ehaA
ExpressionBacterialAvailable SinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pReg-ROTU4
Plasmid#190669PurposeExpresses an evolved rotundine biosensor in E. coliDepositorInsertROTU4
UseSynthetic BiologyAvailable SinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGfOMT1-GEN4
Plasmid#190612PurposeExpresses an evolved methyltransferase for tetrahydropapaverine biosynthesis in E. coliDepositorInsertGfOMT-GEN4
UseSynthetic BiologyAvailable SinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pReg-GLAU4
Plasmid#190667PurposeExpresses an evolved glaucine biosensor in E. coliDepositorInsertGLAU4
UseSynthetic BiologyAvailable SinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pReg-PAP4
Plasmid#190668PurposeExpresses an evolved papaverine biosensor in E. coliDepositorInsertPAP4
UseSynthetic BiologyAvailable SinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pReg-NOS4
Plasmid#190670PurposeExpresses an evolved noscapine biosensor in E. coliDepositorInsertNOS4
UseSynthetic BiologyAvailable SinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVY-3 sfGFP-tag3
Plasmid#194618Purposefluorescent proteins with cationic peptide tags to promote biomolecular condensate formation in E. coliDepositorInsertsfGFP-K(GGSKKRKKR)3
TagsKGGSKKRKKRGGSKKRKKRGGSKKRKKR and MGHHHHHGGAExpressionBacterialPromoterT7Available SinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFLIP37
Plasmid#65505PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-Cit-attR1 and attR2-Cer-cMycExpressionBacterialPromoterT7 promoterAvailable SinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28b-MseLig1
Plasmid#107071PurposeExpresses M. sedula DNA Ligase 1 in E. coliDepositorInsertDNA ligase 1
TagsHis6 tagExpressionBacterialPromoterT7 promoterAvailable SinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-G2K
Plasmid#70169Purposebacterial expression of G2K (d-glycerate 2-kinase)DepositorInsertgarK/G2K
Tags6xHIS and V5ExpressionBacterialAvailable SinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_RcCHAD
Plasmid#127782PurposeExpresses RcCHAD in E. coli cellsDepositorInsertRcCHAD
Tags6xHis tag + TEV cleavage siteExpressionBacterialAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAtLCYbSK
Plasmid#53588PurposeProduces an active Arabidopsis thaliana lycopene beta-cyclase enzyme in E. coli. Also known as pAt9SK.DepositorAvailable SinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
MS2-ecDHFR
Plasmid#65859PurposeExpresses RNA-binding protein MS2 with eDHFR fusion tagDepositorAvailable SinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDW115
Plasmid#70062Purposeartificial transcription template "pause ablation"DepositorInsertlambda PR' A+2G T+6G - repeat cassette - E. coli rpoB - lambda TR'
Available SinceJan. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1 mouse Tankyrase1 5ARC (171–961)
Plasmid#132638Purposeexpresses mouse Tankyrase1 5ARC (171–961) in E. coliDepositorInsertTankyrase1 (171–961)
ExpressionBacterialAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC19-P70a-Bottom-T
Plasmid#87313PurposeExpresses the "Bottom" transcript of the Split-Broccoli system under the control of the E. coli sigma 70 promoter.DepositorInsertSplit-Broccoli system "Bottom" transcript
UseSynthetic BiologyExpressionBacterialPromoterP70aAvailable SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTH07
Plasmid#70063Purposeartificial transcription template "distal pause"DepositorInsertlambda PR' - repeat cassette - E. coli rpoB with inserted pause - lambda TR'
Available SinceJan. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
36INS-J23100_GB
Plasmid#78669Purpose36INS-J23100_GB Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_J23100. [G:36INS-J23100:B]DepositorInsertInsulated J23100 Promoter
UseSynthetic BiologyAvailable SinceAug. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
36INS-J23100_FB
Plasmid#78668PurposeInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_J23100. [F:36INS-J23100:B]DepositorInsertInsulated J23100 Promoter
UseSynthetic BiologyAvailable SinceAug. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
36INS-J23100_EB
Plasmid#78667Purpose36INS-J23100_EB Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_J23100. [E:36INS-J23100:B]DepositorInsertInsulated J23100 Promoter
UseSynthetic BiologyAvailable SinceAug. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
36INS-J23100_AB
Plasmid#78666PurposeInsulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected.Modified from BBa_J23100. [A:36INS-J23100:B]DepositorInsertInsulated J23100 Promoter
UseSynthetic BiologyAvailable SinceAug. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHBFD1
Plasmid#53565PurposeProduces an active HBFD (carotenoid 4-hydroxy-β-ring 4-dehydrogenase) enzyme in E. coli.DepositorInserthbfd1 cDNA
ExpressionBacterialPromoterlacAvailable SinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRL1058
Plasmid#70686PurposeTn5-based plasmid for transposition in Anabaena sp. strain PCC 7120. Bears an oriV, so when excised from a chromosome or megaplasmid it can carry its neighboring sequences when returned to E. coli.DepositorInsertTransposon Tn5-1058
Available SinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-CylA
Plasmid#48198PurposeExpresses CylA (fatty acyl-AMP ligase) in E. coliDepositorInsertCylA
Tags6x His tagExpressionBacterialPromoterT7Available SinceMarch 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
T7-AglC-His6
Plasmid#89726PurposeExpresses AglC from M. voltae with an N-terminal T7 tag and a C-terminal His6 tag. Plasmid has codons optimized for E. coli expression.DepositorInsertAglC
TagsHis6 and T7ExpressionBacterialAvailable SinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
T7-AglK-His
Plasmid#89714PurposeExpresses AglK from M. voltae with an N-terminal T7 tag and a C-terminal His6 tag. Plasmid has codons optimized for E. coli expression.DepositorInsertAglK
TagsHis6 and T7ExpressionBacterialAvailable SinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
p70rg 1
Plasmid#17828DepositorInsertE. coli optimized red flourescent protein (RFPec), GFPuv
UseSynthetic BiologyExpressionBacterialAvailable SinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pUC19-P70a-Top-T
Plasmid#87312PurposeExpresses the "Top" transcript of the Split-Broccoli system under the control of the E. coli sigma 70 promoter.DepositorInsertSplit-Broccoli system "Top" transcript
UseSynthetic BiologyExpressionBacterialPromoterP70aAvailable SinceJan. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28-p53 (Dog 1-77)
Plasmid#62060PurposeExpression of Dog p53 (residues 1-77) in e. coliDepositorInsertp53
TagsHisExpressionBacterialMutationcontains TAD residues 1-77PromoterT7Available SinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only