We narrowed to 9,280 results for: Pol
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Plasmid#227122PurposeBarcoded assay, UAS sensor for split TEV assays; barcode BC0411DepositorInsert10x clustered UAS element linked to CMV minimal promoter driving barcode 0411 and a luciferase reporter gene
ExpressionMammalianAvailable SinceNov. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_10xUAS-CMVmin-BC0405-luc2
Plasmid#227118PurposeBarcoded assay, UAS sensor for split TEV assays; barcode BC0405DepositorInsert10x clustered UAS element linked to CMV minimal promoter driving barcode 0405 and a luciferase reporter gene
ExpressionMammalianAvailable SinceNov. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_10xUAS-CMVmin-BC0408-luc2
Plasmid#227120PurposeBarcoded assay, UAS sensor for split TEV assays; barcode BC0408DepositorInsert10x clustered UAS element linked to CMV minimal promoter driving barcode 0408 and a luciferase reporter gene
ExpressionMammalianAvailable SinceNov. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_10xUAS-CMVmin-BC0441-luc2
Plasmid#227129PurposeBarcoded assay, UAS sensor for split TEV assays; barcode BC0441DepositorInsert10x clustered UAS element linked to CMV minimal promoter driving barcode 0441 and a luciferase reporter gene
ExpressionMammalianAvailable SinceNov. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_CRE-CMVmin-BC0498-luc2
Plasmid#227134PurposeBarcoded assay, cAMP/Ca2+ sensor; barcode BC0498DepositorInsert6x clustered CRE element linked to CMV minimal promoter driving barcode 0498 and a luciferase reporter gene
ExpressionMammalianAvailable SinceNov. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_10xUAS-CMVmin-BC0440-luc2
Plasmid#227128PurposeBarcoded assay, UAS sensor for split TEV assays; barcode BC0440DepositorInsert10x clustered UAS element linked to CMV minimal promoter driving barcode 0440 and a luciferase reporter gene
ExpressionMammalianAvailable SinceNov. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_CRE-CMVmin-BC0504-luc2
Plasmid#227135PurposeBarcoded assay, cAMP/Ca2+ sensor; barcode BC0504DepositorInsert6x clustered CRE element linked to CMV minimal promoter driving barcode 0504 and a luciferase reporter gene
ExpressionMammalianAvailable SinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_10xUAS-CMVmin-BC0442-luc2
Plasmid#227130PurposeBarcoded assay, UAS sensor for split TEV assays; barcode BC0442DepositorInsert10x clustered UAS element linked to CMV minimal promoter driving barcode 0442 and a luciferase reporter gene
ExpressionMammalianAvailable SinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
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p3E-EF1a-pA (JDW 1222)
Plasmid#224526PurposeA Gateway compatible 3' entry clone containing rat EF-1a polyADepositorInsertEF-1a polyA
UseGateway cloningAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-H1-shSRGAP2B/C#1-hsynapsin-EGFP
Plasmid#220796PurposeshRNA plasmid against human SRGAP2B/C genes (#1) with EGFP expression in neuronsDepositorInsertEGFP
Available SinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Fgß-Coh7-ddFLN4-ELP-HIS-ybbR
Plasmid#169135PurposeE. coli expression of Coh7 domain containing Fgß tag, ybbR tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements.DepositorInsertCoh7
Tags3xELP linker, 6x Histag, Fgβ peptide, ddFLN4 tag,…ExpressionBacterialAvailable SinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-PODXL FBM*
Plasmid#195220PurposeMammalian expression vector containing GFP tagged Podocalyxin. With H477/R479/S481>AAA mutation.DepositorAvailable SinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-PODXL S481A
Plasmid#195223PurposeMammalian expression vector containing GFP tagged Podocalyxin. PKC phosphorylation-deficient mutant.DepositorAvailable SinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPSt_35Spr-GFP-t35S
Plasmid#203592PurposeFully assembled Plant STARR-seq plasmid with the 35S terminator.DepositorInsertsCaMV 35S promoter + Zm00001d041672 5'UTR
eGFP
CaMV 35S terminator
ExpressionPlantAvailable SinceSept. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGGI_Myr-YFP-MiniTurboID
Plasmid#222432PurposePotential plasma membrane control.DepositorInsertMiniTurboID (BirA mutant)
UseGolden gate / green gate compatible cloning vectorTagsLinker, Myr, and YFPMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Available SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGM9
Plasmid#220167PurposeExpresses a reporter for an alpha-helical OMM proteinDepositorAvailable SinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pGM295
Plasmid#220183PurposeExpresses exogenous wild-type SEC61B with a C-terminal sfGFP tagDepositorAvailable SinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
p2701-AGER-tdTomato
Plasmid#216470Purposedonor vector for targeting a tdTomato reporter to the human AGER locus at the endogenous ATG start siteDepositorInserttdTomato
UseCRISPRAvailable SinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
p2707-pHAGE-Ef1aL-dsRed-UBC-tagBFP
Plasmid#216476Purposelentiviral dual expression of dsRed and tagBFPDepositorInsertsdsRed
tagBFP
UseLentiviralPromoterEF1aL and UBCAvailable SinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
MCAM-BirA*-HA
Plasmid#214472PurposeLentiviral vector for the expression of MCAM-BirA*-HA fusion protein in mammalian cells.DepositorInsertMCAM (MCAM Human)
UseLentiviral; GatewayTagsBirA(R118G) and HA tagExpressionMammalianPromoterEF-1aAvailable SinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-CUP1-His6-CUbo(K48R)-GFP
Plasmid#212793PurposeCopper-inducible expression of the artificial test substrate His6-CUbo(K48R)-GFP in budding yeastDepositorInsertHis6-CUbo(K48R)-GFP
UseIntegrative vectorExpressionYeastMutationCUb(K48R)PromoterCUP1Available SinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-CUP1-His6-CUbo(K63R)-GFP
Plasmid#212794PurposeCopper-inducible expression of the artificial test substrate His6-CUbo(K63R)-GFP in budding yeastDepositorInsertHis6-CUbo(K63R)-GFP
UseIntegrative vectorExpressionYeastMutationCUb(K63R)PromoterCUP1Available SinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-ADH-NUbo-E3(48) aa24-150-VSV
Plasmid#212747PurposeConstitutive expression of NUbo-E3(48)ΔU7BD-VSV in budding yeast, inactive E3(48)DepositorInsertE3(48) aa24-150
UseIntegrative vectorTagsNUbo and VSVExpressionYeastMutationCue1 ΔU7BDPromoterADH1Available SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-ADH-myc-E3(1) C885A-CUbo-VSV
Plasmid#212738PurposeConsitutive expression of myc-E3(1) C885A-CUbo-VSV in budding yeast, catalytic inactive E3(1)DepositorInsertE3(1)
UseIntegrative vectorTagsCUbo-NLS-VSV and mycExpressionYeastMutationHOIP(C885A)PromoterADH1Available SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7
Plasmid#212805PurposeConstitutive or doxycycline-inducible expression of NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7 in mammalian cellsDepositorInsertNUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7
ExpressionMammalianAvailable SinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
PABPC1_544-626_pGEX6P1
Plasmid#209790PurposeBacterial expression of PABPC1 Mlle domainDepositorInsertMlle domain of PABPC1 (PABPC1 Human)
TagsGSTExpressionBacterialMutationaa 544-626 onlyPromotertacAvailable SinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
rUBR5_2383-2442_pGEX6P1
Plasmid#209791PurposeBacterial expression of UBR5 Mlle domainDepositorInsertMlle domain of UBR5 (Ubr5 Rat)
TagsGSTExpressionBacterialMutationaa 2383-2442 onlyPromotertacAvailable SinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
LARP1_323-509
Plasmid#209800PurposeBacterial expression of human LARP1 La-moduleDepositorInsertLARP1 La-module (LARP1 Human)
Tags6xHisExpressionBacterialMutationaa 323-509 onlyPromoterT7Available SinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
LARP1_323-410
Plasmid#209801PurposeBacterial expression of human LARP1 LaM domainDepositorAvailable SinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
SUMO-Twinkle 43-372
Plasmid#205054PurposeTWNK protein expressionDepositorInsertTWNK (TWNK Human)
ExpressionBacterialMutationLacks Mitochondrial localization signal and C ter…Available SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-HA_aa 321-843 REV1
Plasmid#201587PurposeExpresses the catalytic domain (aa 321-843) of human DNA repair protein REV1 with a HA tag in mammalian cellsDepositorInsertDNA repair protein REV1 (REV1 Human)
TagsHAExpressionMammalianMutationExpresses amino acids 321-843 of DNA repair prote…Available SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_WT RAD23B_aa 80-409 (delta UBL domain)
Plasmid#201545PurposeExpresses a mutant form of human RAD23B that lacks the N-terminal UBL domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23BDepositorInsertUV excision repair protein RAD23 homolog B (RAD23B Human)
TagsFLAGExpressionMammalianMutationLacks N-terminal residues 1-79Available SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_WT RAD23B_delta aa 188-228 (delta UBA1 domain)
Plasmid#201546PurposeExpresses a mutant form of human RAD23B that lacks the UBA1 domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23BDepositorInsertUV excision repair protein RAD23 homolog B (RAD23B Human)
TagsFLAGExpressionMammalianMutationLacks residues 188-228Available SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_aa 1-363 RAD23B (delta UBA2 domain)
Plasmid#201548PurposeExpresses a mutant form of human RAD23B that lacks the UBA2 domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23BDepositorInsertUV excision repair protein RAD23 homolog B (RAD23B Human)
TagsFLAGExpressionMammalianMutationLacks C-terminal residues 364-409Available SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBactin-AcGFP-CI
Plasmid#196849PurposeExpression of green fluorescent reporter AcGFP driven by the strong Beta-actin promoterDepositorInsertAcGFP
ExpressionMammalianMutationNone (wt)PromoterChicken bactin (plus Chicken bactin intron)Available SinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET19-TdTdelta5cys
Plasmid#207465PurposeBacterial expression of Terminal deoxynucleotidyl transferase (TdT) surface-cysteine free variantDepositorInsertTdTdelta5cys (Dntt Mouse)
Tags10xHisExpressionBacterialMutationCys188Ala, Cys216Ser, Cys302Ala, Cys378Ala, and C…Available SinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-3UTR
Plasmid#202550PurposeReady for cloning and heterologous expression of genes in mammalian cells with protein export to cultivation mediaDepositorTypeEmpty backboneTagsATG initiation codon followed by an IGKV3 signal …ExpressionMammalianPromoterCMVAvailable SinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-lox-inv[Talpha1-iCre-pA]-lox-mOrange2-C1
Plasmid#196877PurposeNeuron-specific expression of mOrange2 reporterDepositorInsertmOrange2
UseCre/LoxExpressionMammalianPromoterQuimeric CAGAvailable SinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBactin-td-mCherry-CI
Plasmid#196851PurposeExpression of a tandem (td) red fluorescent reporter mCherry driven by the strong Beta-actin promoterDepositorInserttd-mCherry
ExpressionMammalianMutationNone (wt)PromoterChicken bactin (plus Chicken bactin intron)Available SinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-KIF1aMotor-mScarlet-FKBP
Plasmid#191336PurposeExpresses the fluorescently labeled plus-end directed KIF1a motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRBDepositorAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEE005
Plasmid#176762PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert101
Tags6xHisExpressionBacterialPromoterT7Available SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-FKBP-mScarlet-KIFC1motorN593K
Plasmid#191335PurposeExpresses the fluorescently labeled minus-end directed KIFC1 motor-deficient motor domain fused to FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRBDepositorInsertFKBP-mScarlet-KIFC1N593K (KIFC1 Human)
ExpressionMammalianMutationmotor domain aa 125-673; N593KAvailable SinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEE070
Plasmid#176827PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert709
Tags6xHisExpressionBacterialPromoterT7Available SinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEE071
Plasmid#176828PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert710
Tags6xHisExpressionBacterialPromoterT7Available SinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEE072
Plasmid#176829PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert711
Tags6xHisExpressionBacterialPromoterT7Available SinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEE073
Plasmid#176830PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert712
Tags6xHisExpressionBacterialPromoterT7Available SinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only