We narrowed to 13,459 results for: 109
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Plasmid#131109PurposeExpresses KSHV ORF66 a.a. 1-429 (full length) C427A mutant with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
Tags2xStrepExpressionMammalianMutationORF66 a.a. 1-429 (full-length); cysteine 427 muta…PromoterCMVAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mG3_CH2charge
Plasmid#105858PurposeExpression plasmid coding for mutated heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. Introduced mutatnions modify charge of CH2 domain.DepositorInsertIgG3 heavy chain (Ighg3 Mouse)
ExpressionMammalianMutationmutated heavy chain of mouse IgG3, muatations: Hi…PromoterhEF1-HTLV promAvailable SinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-ARL4D
Plasmid#67468PurposeMammalian expression of hArl4D with stop codon (native expression)DepositorInsertARL4D (ARL4D Human)
ExpressionMammalianMutationbp 272 A to C; N91T mutation; bp 327 T to G; sil…PromoterCMVAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-ARL4D-V5-His6
Plasmid#67447PurposeMammalian expression of hArl4D with C-term V5 and His6 tagDepositorInsertARL4D (ARL4D Human)
TagsHis and V5ExpressionMammalianMutationbp 272 A to C; N91T mutation; bp 327 T to G; sil…PromoterCMVAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-ARL4D-V5-His6
Plasmid#67426PurposeExpression of hArl4D with C-term V5 and His6 tag in E. coliDepositorInsertARL4D (ARL4D Human)
TagsHis and V5ExpressionBacterialMutationbp 272 A to C; N91T mutation; bp 327 T to G; sil…PromoterT7/lacOAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-ARL4D-GFP
Plasmid#67405PurposeMammalian expression of hArl4D with C-term GFP tagDepositorInsertARL4D (ARL4D Human)
TagsGFPExpressionMammalianMutationbp 272 A to C; N91T mutation; bp 327 T to G; sil…PromoterCMVAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARL4D-no stop
Plasmid#67337PurposehArl4D without stop codon in Gateway Entry vectorDepositorInsertARL4D (ARL4D Human)
UseGateway entry vectorExpressionBacterialMutationbp 272 A to C; N91T mutation; bp 327 T to G; sil…PromoterNoneAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARF4-no stop
Plasmid#67311PurposehArf4 without stop codon in Gateway Entry vectorDepositorInsertARF4 (ARF4 Human)
UseGateway entry vectorExpressionBacterialMutationV53A, L123P and M134I mutations were unintended P…Available SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
I-GCAG
Plasmid#43109DepositorInsertI-GCAG
UseBacterial cloningAvailable SinceJuly 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLV-CMV-EGFP-PolB-Hygro
Plasmid#176056PurposeEGFP fused to the N-terminus of PolB & a hygromycin resistance cassetteDepositorAvailable SinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458-Dual-Guide-Donor-ShuttleOnly
Plasmid#171098PurposeDonor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.DepositorTypeEmpty backboneUseCRISPRMutationXbaI site added upstream of U6 promotor. GtoC mu…Available SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-CAG-3xNLS-mScarlet
Plasmid#191098PurposeTo express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methodsDepositorInsert3xNLS-mScarlet
UseAAV and Synthetic BiologyTagsThree repeats of the nuclear localizzation seque…ExpressionMammalianMutationOptimized to human codon usagePromoterCMV enhancer and CAGAvailable SinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOPINE GFP nanobody:Halo:His6
Plasmid#111090PurposeBacterial expression of a fusion protein consisting of a GFP nanobody (Dr. Brett Collins, Addgene plasmid # 49172), the Halo tag and a His6 tag.DepositorInsertGFP Nanobody
TagsHalo and His6ExpressionBacterial, Insect, and Mamm…PromoterT7 promoterAvailable SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FGFR4-V5/HIS
Plasmid#201109Purposeexpression of human FGFR4 receptor tyrosine kinase in mammalian cellsDepositorInserthuman FGFR4 receptor tyrosine kinase, full length, wildtype (FGFR4 Human)
TagsV5/HisExpressionMammalianPromoterCMVAvailable SinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-CAG-3xNLS-vsfGFP-0
Plasmid#191097PurposeTo express a bright green nanobody FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methodsDepositorInsert3xNLS-vsfGFP-0
UseAAVTagsGreen fluorescent protein bound to enhancer nanob…ExpressionMammalianMutationOptimized to Human codon usagePromoterCMV and CAGAvailable SinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3-OGDH_wobble
Plasmid#110938PurposeOGDH ORF containing silent wobble mutations insensitive to corresponding shRNAsDepositorInsertOGDH wobble cDNA (OGDH Human)
UseLentiviralMutation15 silent wobble mutations corresponding to three…PromoterSV40Available SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-CAG-3xNLS-AausFP1
Plasmid#191096PurposeTo express a bright green FP to label eucaryotic cell nuclei. To be used in tissue-clearing methods and other fluorescent microscopy methodsDepositorInsert3xNLS-AausFP1
UseAAV and Synthetic BiologyTagsThree repeats of the nuclear localizzation seque…ExpressionMammalianMutationOptimized to Human codon usagePromoterCMV enhancer and CAGAvailable SinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-RaichuEV-Rac1(Y40C)
Plasmid#164903PurposeExpresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation Y40C in the Rac1 domain. More useful than T17N mutation due to less harmful to endogenous signaling.DepositorInsertRaichuEV-Rac1 (RAC1 Human)
ExpressionMammalianMutationY40C mutation in the Rac1 domain whcih make the s…Available SinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCasper4-g-osk-TRICK
Plasmid#71244Purposeoskar translation biosensor with 12x copies of modified PP7 stem loops and 6xMS2 stem loops. . full genomic region, used for P element-mediated germline transformation in Drosophila.DepositorInsertoskar (osk Fly)
UseP element-mediated germline transformationTags12xPP7 and 6xMS2ExpressionInsectPromoteroskAvailable SinceDec. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTUB1-Sth.dCas9-P2A-CAT-T2A-TagBFP
Plasmid#171090PurposeCRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus thermophilus (CRISPR1 locus) in Toxoplasma gondii. Suitable for generating stable cell lines.DepositorInsertsSth1 dCas9
Chloramphenicol acetyltransferase
TagBFP
UseCRISPR; Toxoplasma gondii expressionTags3xFLAG, P2A peptide, SV40 NLS, T2A peptide, and n…MutationD9A, H599APromoterTUB1 (Toxoplasma gondii), transcriptionally linke…Available SinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only