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pSLQ1661-sgMUC4-E3(F+E)

Plasmid

#51025

Purpose

Lentiviral vector that contains an optimized S. pyogenes sgRNA targeting the repetitive sequence of human MUC4 exon 3

Depositor

Bo Huang, Stanley Qi

Article

Chen et al Cell. 2013 Dec 19;155(7):1479-91. doi: 1

Insert

optimized sgRNA (MUC4 Mouse, Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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hsDCX-pHAT

Plasmid

#83928

Purpose

hsDCX-pHAT N-terminal 6xHis-tag, C-terminal Strep-tagII

Depositor

Gary Brouhard

Article

Bechstedt et al Dev Cell. 2012 Jul 17;23(1):181-92. doi:

Insert

human Doublecortin isoform b (DCX Human)

Tags

6-His tag and Strep-TagII

Expression

Bacterial

Available Since

Dec. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pJMP3

Plasmid

#79875

Purpose

Bacillus subtilis sgRNA expression vector; integrates into thrC

Depositor

Carol Gross, Jason Peters, Stanley Qi

Article

Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

Insert

sgRNA RR1

Use

CRISPR

Expression

Bacterial

Promoter

veg

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry

Plasmid

#75470

Purpose

Flp-dependent Chr2-mCherry encoded in an AAV vector for rAAV production and expression in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

Has Service

AAV1

Insert

Reversed ChR2(H134R)-mCherry

Use

AAV and Synthetic Biology

Expression

Mammalian

Promoter

CAG

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-mClover3-anti-mouse-IgG(kappa)-nanobody

Plasmid

#138128

Purpose

Expresses GST-tagged mClover3-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

Insert

mClover3-anti-mouse-IgG(kappa)-nanobody

Tags

GST

Expression

Bacterial

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5 c-Fyn

Plasmid

#16032

Depositor

Filippo Giancotti

Article

Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

Insert

c-Fyn (FYN Human)

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 3xFlag-JNK1a1

Plasmid

#47574

Purpose

Bacterial expression plasmid for GST-3xFlag-tagged JNK1a1

Depositor

Kevin Janes

Article

Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

Insert

JNK1a1 (MAPK8 Human)

Tags

3xFlag and GST

Expression

Bacterial

Available Since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
pBJ FLAG-hPar1

Plasmid

#53226

Purpose

mammalian expression of human Par1 with N-terminal FLAG tag

Depositor

Shaun Coughlin

Article

Ishii et al J Biol Chem. 1993 May 5. 268(13):9780-6.

Insert

Par1 (F2R Human)

Tags

FLAG and bovine prolactin signal sequence (MDSKGS…

Expression

Mammalian

Mutation

Deletion of aa1-39 (native signal peptide)

Promoter

SV40

Available Since

Aug. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pEF-GalNAc-T2-msGFP2

Plasmid

#140164

Purpose

Expresses the Golgi marker GalnacT2-msGFP2

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

GalNAc-T2-msGFP2 (GALNT2 Human)

Expression

Mammalian

Promoter

EF-1a

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-Sestrin1 isoform1

Plasmid

#72594

Purpose

overexpression, codon optimized for mammalian expression

Depositor

David Sabatini

Article

Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

Insert

SESN1 (SESN1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

codon optimized for mammalian expression, see dep…

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
tet-pLKO shGATA6 puro

Plasmid

#72615

Purpose

Expresses an inducible short hairpin targeting human GATA6 sequence

Depositor

Kevin Janes

Article

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

short hairpin GATA binding protein 6

Use

Lentiviral

Tags

None

Expression

Mammalian

Promoter

TRE Tight

Available Since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pC001 - huLshC2C2-MBP for bacterial expression

Plasmid

#79150

Purpose

Expresses human codon-optimized LshC2c2 for purification in E. coli

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

Insert

C2c2

Expression

Bacterial

Promoter

T7

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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AgDD

Plasmid

#78289

Purpose

create protein aggregates in living cells

Depositor

Thomas Wandless

Article

Miyazaki et al Nat Commun. 2016 May 27;7:11689. doi: 10

Insert

NES-L106P-sfGFP (FKBP1A Human)

Tags

MLALKLAGLDI

Expression

Mammalian

Promoter

pCAG

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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Synapsin sHRPa-NRX in FSW lentiviral vector

Plasmid

#73147

Purpose

Large fragment of split HRP fused to the extracellular terminus of neurexin3B. This is a lentiviral plasmid, but can also be used for transfections. Synapsin promoter.

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

sHRPa-NRX

Use

Lentiviral

Tags

2x HA tag and Flag tag

Expression

Mammalian

Promoter

Synapsin

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pDrVSP-IRES2-EGFP

Plasmid

#80333

Purpose

Expresses Danio rerio VSP with separated EGFP in mammalian cell

Depositor

Yasushi Okamura

Article

Hossain et al J Biol Chem. 2008 Jun 27;283(26):18248-5

Insert

Daino rerio voltage-sensing phosphatase (tpte Zebrafish)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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His-GFP-Clathrin

Plasmid

#170858

Purpose

Mammalian expression of His-GFP-Clathrin.

Depositor

Justin Taraska

Article

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

Clathrin light polypeptide (Lca) (Clta Mouse)

Tags

(His)6 and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG2LMKOSimO

Plasmid

#20866

Depositor

Keisuke Kaji

Article

Kaji et al Nature. 2009 Apr 9;458(7239):771-5. doi:

Insert

c-Myc-F2A-Klf4-T2A-Oct4-E2A-Sox2-ires-mOrange (Myc Mouse)

Expression

Mammalian

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only

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GFP-JNK1

Plasmid

#86830

Purpose

mammalian expression of GFP-JNK1

Depositor

Rony Seger

Article

Zehorai et al Mol Cell Biol. 2014 Jan;34(2):259-70. do

Insert

JNK1 (MAPK8 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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N-Myc-53BP1 WT pLPC-Puro

Plasmid

#19836

Depositor

Titia de Lange

Article

Dimitrova et al Nature. 2008 Nov 27;456(7221):524-8. doi

Insert

p53 Binding Protein 1 (TP53BP1 Human)

Use

Retroviral

Tags

Myc

Available Since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only

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MSCV Myc IRES GFP

Plasmid

#18770

Depositor

Scott Lowe

Article

Hemann et al Nature. 2005 Aug 11. 436(7052):807-11.

Insert

Myc (Myc Mouse)

Use

Retroviral

Tags

IRES GFP

Expression

Mammalian

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only