We narrowed to 24,482 results for: Spr

1. Lenti_sgRNA(MS2)_zsGreen1

   Plasmid

   #138463

   Purpose

   lenti sgRNA cloning backbone with MS2 loops and EF1a-zsGreen1

   Depositor

   Jian Xu

   Article

   Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

   Insert

   zsGreen1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   May 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRH2521

   Plasmid

   #84380

   Purpose

   Expression of sgRNA from a imyc promoter (Pimyc)

   Depositor

   Robert Husson

   Article

   Singh et al Nucleic Acids Res. 2016 Jul 12. pii: gkw

   Insert

   sgRNA cloning site
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLH-nmsgRNA1.1

   Plasmid

   #64115

   Purpose

   Vector for expression of Nm sgRNA1.1 in mammalian cells

   Depositor

   Thoru Pederson

   Article

   Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

   Insert

   Nm sgRNA1.1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pWRS_1001

   Plasmid

   #192205

   Purpose

   lentiviral expression of SpCas9 and two pol III promoters for dual expression of sgRNAs

   Depositor

   William Sellers

   Article

   Li et al Nat Commun. 2022 May 5;13(1):2469. doi:

   Type

   Empty backbone

   Use

   CRISPR, Lentiviral, and RNAi

   Available Since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE-TO-nmdCas9-3XGFP

   Plasmid

   #64109

   Purpose

   Expression of Nm dCas9-3XGFP in mammalian cells

   Depositor

   Thoru Pederson

   Article

   Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

   Insert

   Nm dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3XsfGFP

   Expression

   Mammalian

   Promoter

   CMV-TO

   Available Since

   April 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3.1- dCas9-2xNLS-EGFP

   Plasmid

   #74710

   Purpose

   Expresses dCas9-2xNLS-EGFP in mammalian cells

   Depositor

   Eugene Yeo

   Article

   Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

   Insert

   dCas9-2xNLS-EGFP
   

   Use

   CRISPR

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330GFP-hU6-gRNA-hSpCas9

   Plasmid

   #128385

   Purpose

   GFP positive gRNA/hSpCas9 construct

   Depositor

   Budd A. Tucker

   Article

   Giacalone et al Curr Protoc Stem Cell Biol. 2018 Feb 28;

   Insert

   GFP, gRNA and hSpCas9
   

   Use

   CRISPR

   Available Since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJAT10

   Plasmid

   #204293

   Purpose

   For marking CRISPR HDR integrant with DsRed expressed in flight muscles.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   MHC-DsRed
   

   Use

   CRISPR

   Promoter

   MHC

   Available Since

   Aug. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHAGE-EFS-PCP-GFPnls

   Plasmid

   #121938

   Purpose

   CRISPR-Sirius plasmid

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

   Insert

   PCP-GFPnls
   

   Use

   Lentiviral

   Tags

   sfGFP

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. p15A_SpyCas9_CmR

    Plasmid

    #119162

    Purpose

    Expresses S. pyogenes Cas9

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    Cas9 from S. pyogenes (NEWENTRY )
    

    Use

    Crispr

    Available Since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJAT30

    Plasmid

    #204294

    Purpose

    For marking CRISPR HDR integrant with DsRed expressed in flight muscles. Second U6 promoter for using two gRNAs.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    MHC-DsRed
    

    Use

    CRISPR

    Promoter

    MHC

    Available Since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTPGI_dCas9_VP64

    Plasmid

    #49013

    Purpose

    encodes yeast-optimized dCas9_VP64 synthetic transcription factor

    Depositor

    Timothy Lu

    Article

    Farzadfard et al ACS Synth Biol. 2013 Sep 11.

    Insert

    dCas9_VP64 (codon-optimized for expression in S. cerevisiae)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40 NLS

    Expression

    Yeast

    Promoter

    pTPGI (galactose+aTc inducible)

    Available Since

    Jan. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBx-Spas-sgRNA-Gm

    Plasmid

    #105232

    Purpose

    Plasmid containing S. pasteurianus sgRNA with no 20bp target (Empty control) Gentamycin marker for P. aeruginosa

    Depositor

    Kristala Prather

    Article

    Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

    Insert

    S. pasteurianus sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJAT39

    Plasmid

    #204289

    Purpose

    For marking CRISPR HDR integrant with EGFP expressed in flight muscles.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    MHC-EGFP+H3:H26
    

    Use

    CRISPR

    Promoter

    MHC

    Available Since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRISPomyces-Sth1Cas9

    Plasmid

    #129552

    Purpose

    Contains codon-optimized Sth1Cas9 and gRNA for streptomyces

    Depositor

    Fong Tian Wong, Mingzi M. Zhang

    Article

    Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

    Insert

    Sth1Cas9
    

    Expression

    Bacterial

    Promoter

    rpsL(XC)-BbsI

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLH-stsgRNA3.1

    Plasmid

    #64118

    Purpose

    Vector for expression of St1 sgRNA3.1 in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    St1 sgRNA3.1
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. Lenti_MCP-VP64_Hygro

    Plasmid

    #138458

    Purpose

    lenti vector encoding the MS2-VP64 with a 2A Hygro resistance marker

    Depositor

    Jian Xu

    Article

    Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

    Insert

    vp64
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    May 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDHt/sk-PC

    Plasmid

    #92125

    Purpose

    Expression of codon-optimized Cas9 with Ppdc promoter in Trichoderma reesei

    Depositor

    Zhihua Zhou

    Article

    Liu et al Cell Discov. 2015 May 12;1:15007. doi: 1

    Insert

    Cas9
    

    Use

    CRISPR; Trichoderma reesei expression

    Promoter

    Ppdc

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKHH030

    Plasmid

    #89358

    Purpose

    hU6 sgRNA cloning vector for CDKO system

    Depositor

    Michael Bassik

    Article

    Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103

    Insert

    GFP, Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. EC59103

    Plasmid

    #191785

    Purpose

    LbCas12a expression in plants

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    AtUbi10::Cas12a+ 8 introns-T-E9
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D156R

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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