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Plasmid#138582PurposeExpresses fluorescent ALIX in mammalian cellsDepositorInsertALIX (PDCD6IP Human)
UseLentiviralTagsGFP and h30ExpressionMammalianMutationPromoterTREtightAvailable sinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRT029
Plasmid#127969Purposedouble-DHFR-degron controlled expression of dCas9-BFP-KRAB from the CLYBL locusDepositorInsertCLYBL-CAG-DHFR-dCas9-BFP-KRAB-DHFR
UseTALENTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMSP2N2
Plasmid#29520DepositorInsertMSP2N2
UseTags7-His tagExpressionBacterialMutationtandem MSP; His-tag on N-terminus followed by spa…PromoterAvailable sinceMay 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET His6 GFP TEV LIC cloning vector (1GFP)
Plasmid#29663DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTags6xHis, GFP, and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacO (lactose/IPTG inducible)Available sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-Cre
Plasmid#55636PurposeExpresses Cre in Mammalian CellsDepositorHas ServiceAAV RetrogradeInsertCre
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEf1aAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX-KG-D4H*-mCherry
Plasmid#134604Purposefluorescent cholesterol-binding probeDepositorInsertD4H*
UseTagsGST and mCherryExpressionBacterialMutationY415A and A463WPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBA904
Plasmid#122238PurposeLentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region.DepositorInsertsgRNA with cs1 in stem loop 2 of the constant region
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-SaCas9-U6-sgRNA
Plasmid#124844PurposeVector for Cre-dependent expression of SaCas9DepositorInsertSaCas9, gRNA scaffold
UseAAV, CRISPR, and Mouse TargetingTagsNLS and NLS-3xHAExpressionMutationPromoterAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
Nuclear cpGFP
Plasmid#215707PurposeExpresses cpGFP in the nucleusDepositorInsertcpGFP
UseLentiviralTagsFLAG, HA, and NLSExpressionMammalianMutationcircularly permuted GFPPromoterCMVAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
scAAV-sgRNA-GFP
Plasmid#177935PurposeExpresses sgRNA and can be packaged into AAV particles for somatic delivery into Cas9 transgenic miceDepositorTypeEmpty backboneUseAAV and CRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only