163,777 results

1. Lenti-TurboCas-NLS-Blast

   Plasmid

   #232346

   Purpose

   Lentiviral expression vector for dCas9 fused to miniTurboNLS

   Depositor

   Ali Shilatifard

   Article

   Cenik et al Mol Cell. 2024 Dec 19;84(24):4929-4944.e

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA, miniTurbo, V5 and 3xSV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-DIO-ATLASsnFlp

   Plasmid

   #232352

   Purpose

   Anterograde transsynaptic tracer protein to express in presynaptic glutamatergic neurons that express Cre, Flp payload. Must use with AAV8-fDIO-Reporter viruses infected in postsynaptic cells.

   Depositor

   Don Arnold

   Article

   Rivera et al Nat Methods. 2025 May;22(5):1101-1111. d

   Insert

   ATLASsnFlp
   

   Use

   AAV

   Tags

   ALFA-Tag

   Expression

   Mutation

   Promoter

   hSyn

   Available Since

   May 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR_Gal4UAS_tBFP_PGK_mCherry

   Plasmid

   #79130

   Purpose

   Lentiviral vector - tBFP reporter for Gal4DBDVP64 synNotch receptors with a constitutive mCherry

   Depositor

   Wendell Lim

   Article

   Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

   Insert

   tBFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJR89

   Plasmid

   #140096

   Purpose

   sgRNA constant region and hU6 insert for programmed dual sgRNA cloning. The sgRNA constant region contains a capture sequence (cs1) in the stem loop for direct capture Perturb-seq.

   Depositor

   Jonathan Weissman

   Article

   Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

   Insert

   sgRNA constant region CR3 with cs1 in stem loop and hU6 promoter
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDestTol2CG2--3.5ubb:plin2-tdtomato

   Plasmid

   #172534

   Purpose

   Zebrafish ubiquitin promoter driving zebrafish PLIN2-tdTOMATO fusion (lipid droplet reporter) with cmlc2:eGFP as a transgenesis marker

   Depositor

   Richard White

   Article

   Lumaquin et al Elife. 2021 Jun 11;10. pii: 64744. doi:

   Insert

   -3.5ubb:plin2-tdtomato
   

   Use

   Tags

   Expression

   Mutation

   S80R, A81S, E144D, I148F and R415H mutations obse…

   Promoter

   zebrafish ubb

   Available Since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJRH-1187 VSVGmut

   Plasmid

   #201913

   Purpose

   Expresses a VSV-G variant (K47Q R354A) defective for LDL-R receptor binding (CAG promoter)

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

   Insert

   VSV-G (K47Q, R354A)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K47Q, R354A

   Promoter

   CAG

   Available Since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-Cre-IRES2-GFP

   Plasmid

   #26646

   Purpose

   Mammalian coexpression of nls-Cre and EGFP from the CAG promoter

   Depositor

   Anjen Chenn

   Article

   Woodhead et al J Neurosci. 2006 Nov 29;26(48):12620-30.

   Insert

   Cre
   

   Use

   Cre/Lox

   Tags

   GFP and IRES2

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Dec. 2, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCW57.1-MAT2A

   Plasmid

   #100521

   Purpose

   Dox-repressible cDNA expression of MAT2A (dox-off/ tet-off all-in-one vector)

   Depositor

   David Sabatini

   Article

   Gu et al Science. 2017 Nov 10;358(6364):813-818.

   Insert

   MAT2A (MAT2A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pTight TRE

   Available Since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330S-2-PITCh

   Plasmid

   #63670

   Purpose

   Expresses Cas9 nuclease and the PITCh-gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available Since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. actc1b-mKate2-P2A-APEX2-VHH[GFP]

    Plasmid

    #126749

    Purpose

    Expression of GFP binding nanobody fused to APEX2 in zebrafish muscle. Parton lab clone DSJ

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    VHH[EGFP]
    

    Use

    Zebrafish muscle expression clone

    Tags

    mKate2-P2A-APEX2

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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