Addgene

We narrowed to 13,587 results for: nts

Showing: 3691 - 3720 of 13587 results

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pcDNA3.2 V5-DEST mir-1-1 reporter

Plasmid

#46646

Purpose

expression of query pri-miRNA

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir-1-1 reporter

Use

RNAi; Expression of query pri-mirna sequences in …

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pRATIO2151

Plasmid

#141300

Purpose

Gateway cloning compatible ratiometric binary vector

Depositor

David Nelson

Article

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Use

Tags

Firefly Luciferase or LUC2 and mScarlet-I

Expression

Plant

Mutation

Promoter

UBQ10 promoter

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pUC19/mChe-C

Plasmid

#183160

Purpose

Plant transient expression vector containing mCherry tag. Unique nucleotide sequences Uc and Ud for cloning at C terminus of mCherry.

Depositor

Qinlong Zhu, Yao-Guang Liu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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YeNL-Actin_pcDNA3

Plasmid

#89521

Purpose

Yellow color variant of bright luminescent protein, fused to actin

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

YeNL

Use

Tags

Actin

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pDGE4

Plasmid

#79443

Purpose

recipient plasmid [multiplexing]; pPcUbi:Cas9, BASTA

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Tags

Expression

Plant

Mutation

BsaI site eliminated

Promoter

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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M-R742X (BF7-2)

Plasmid

#21372

Depositor

Gregory Germino

Article

Hanaoka et al Nature. . 408(6815):990-4.

Insert

autosomal dominant polycystic kidney disease type II (PKD2 Human)

Use

Tags

6xMyc

Expression

Mammalian

Mutation

changed R742 to stop codon

Promoter

Available since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
pDGE506

Plasmid

#153279

Purpose

Intermediate sgRNA array cloning vector position 4

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE505

Plasmid

#153278

Purpose

Intermediate sgRNA array cloning vector position 3

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pFH42

Plasmid

#125881

Purpose

Level0 Cestrum Yellow Leaf Curling Virus CmYLCV Pol II promoter

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Level0 Cestrum Yellow Leaf Curling Virus CmYLCV Pol II promoter

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFH86

Plasmid

#128199

Purpose

improved sgRNA backbone for SpCas9, combine with TaU3 promoter module (pFH33)

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

improved sgRNA backbone for SpCas9, combine with TaU3 promoter module (pFH33)

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pICSL70005

Plasmid

#50335

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

bialaphos resistance cassette (Nos promoter (A. tumefaciens) + phosphinothricin acetyl transferase (S. hygroscopicus) + Nos term

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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tubulin-YeNL_pcDNA3

Plasmid

#89519

Purpose

Yellow color variant of bright luminescent protein, fused to b-tubulin

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

YeNL

Use

Tags

b-tubulin

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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pRDA_381

Plasmid

#179092

Purpose

nuclease Cas expression

Depositor

David Root, John Doench

Article

Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

Insert

IDT-Cas9_v2

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pRDA_157

Plasmid

#179090

Purpose

nuclease Cas expression

Depositor

David Root, John Doench

Article

Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

Insert

HypaCas9

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pRDA_156

Plasmid

#179089

Purpose

nuclease Cas expression

Depositor

David Root, John Doench

Article

Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

Insert

Cas9-VRER

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pRDA_155

Plasmid

#179088

Purpose

nuclease Cas expression

Depositor

David Root, John Doench

Article

Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

Insert

Cas9-VQR

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pRDA_154

Plasmid

#179087

Purpose

nuclease Cas expression

Depositor

David Root, John Doench

Article

Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

Insert

xCas9-3.7

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pRDA_153

Plasmid

#179086

Purpose

nuclease Cas expression

Depositor

David Root, John Doench

Article

Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

Insert

evoCas9

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pRDA_152

Plasmid

#179085

Purpose

nuclease Cas expression

Depositor

David Root, John Doench

Article

Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

Insert

eCas9-1.1

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pRDA_151

Plasmid

#179084

Purpose

nuclease Cas expression

Depositor

David Root, John Doench

Article

Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

Insert

Cas9-HF

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pDH6

Plasmid

#83777

Purpose

Useful for tagging yeast proteins with C-terminal YFP tag; G418 selection

Depositor

Eric Muller

Article

Hailey et al Methods Enzymol. 2002;351:34-49.

Insert

YFP

Use

Tags

YFP

Expression

Yeast

Mutation

S65G, V68L, Q69K, S72A, T203Y mutations added.

Promoter

Available since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR-MXC

Plasmid

#37140

Depositor

Robert Duronio

Article

White et al J Cell Biol. 2011 May 16;193(4):677-94.

Insert

Multi Sex combs (mxc Fly)

Use

Gateway

Tags

Expression

Mutation

Promoter

Available since

Aug. 23, 2012

Availability

Academic Institutions and Nonprofits only
pTRANS_110

Plasmid

#91202

Purpose

Transformation, Type: non-T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

April 23, 2018

Availability

Academic Institutions and Nonprofits only

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pS23·Pm-GFP

Plasmid

#122593

Purpose

Expresses GFP under the regulatory control of Pm. Expression needs additional xyls gene

Depositor

Pablo Ivan Nikel

Article

Volke et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Insert

Pm promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Pm

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pDGE62

Plasmid

#79444

Purpose

recipient plasmid [single nuclease]; p35S:Cas9, nptII

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Tags

Expression

Plant

Mutation

BsaI site eliminated

Promoter

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pRC05

Plasmid

#114684

Purpose

FKBP-TEV-C

Depositor

Matthew Good

Article

Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

Insert

FKBP-TEV-C

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

T7 Promoter

Available since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pTRANS_212

Plasmid

#91111

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: ToLCV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_A3012

Plasmid

#91045

Purpose

Module A, Promoter: PvUbi1, Gene: GFP, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

GFP

Use

Unspecified

Tags

Expression

Mutation

Promoter

PvUbi1

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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OeNL-H2B_pcDNA3

Plasmid

#89527

Purpose

Orange color variant of bright luminescent protein, fused to histone 2B

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

OeNL

Use

Tags

histone 2B

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pICH78133

Plasmid

#50292

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5'UTR, omega (Tobacco Mosaic Virus) + chloroplast transit peptide, RbcS (synthetic)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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