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We narrowed to 18,759 results for: nls

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Showing: 4921 - 4960 of 18759 results

  1. pTK229

    Plasmid
    #71256
    Purpose
    luciferase reposter with 229 bp of the HSV TK promoter
    Depositor
    Peter Cockerill
    Publication
    Hawwari et al J Immunol. 2002 Aug 15;169(4):1876-86.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Thymidine Kinase promoter
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mammalian
    Promoter
    HSV Thymidine Kinase
    Available since
    Nov. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    71256-plasmid-map-sequence-id-127328

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  9. VQR variant

    Plasmid
    #138560
    Purpose
    Expresses human codon-optimized SpCas9-VQR and blasticidin resistance: EFS promoter-VQR-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    VQR
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    D1135V, R1335Q, T1337R
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138560-plasmid-map-sequence-id-274782

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  11. pDD3248 pCS2-mtagBFP2-p2a-NES-arrb2a-(ENLYFQ/S)x3-SV40NLS-sfCherry3C(1-10)

    Plasmid
    #251770
    Purpose
    Blue fluorescent protein-tagged TEVp substrate (three-peat motif) for fluorescent protein reconstitution assay
    Depositor
    Dave Dingal
    Publication
    Lim Suan et al Nat Commun 17, 3257 (2026).
    Insert
    arrestin, beta 2a (arrb2a Zebrafish)
    Tags
    NLS (SV40), sfCherry3C large fragment and mTagBFP…
    Expression
    Mammalian
    Promoter
    CMV IE94
    Available since
    April 9, 2026
    Availability
    Academic Institutions and Nonprofits only
    251770-plasmid-map-sequence-id-504794

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  12. pEJS2784 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-20a CMV-BFP

    Plasmid
    #240514
    Purpose
    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.
    Depositor
    Erik Sontheimer
    Publication
    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10
    Insert
    AcrIIC3
    Tags
    FLAG, hsa-miR-20 Target Sites, and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    240514-plasmid-map-sequence-id-477194

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  13. pEJS2785 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-125a CMV-BFP

    Plasmid
    #240515
    Purpose
    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.
    Depositor
    Erik Sontheimer
    Publication
    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10
    Insert
    AcrIIC3
    Tags
    FLAG, hsa-miR-125a Target Sites, and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    240515-plasmid-map-sequence-id-481869

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  14. pEJS2786 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-151a CMV-BFP

    Plasmid
    #240516
    Purpose
    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.
    Depositor
    Erik Sontheimer
    Publication
    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10
    Insert
    AcrIIC3
    Tags
    FLAG, hsa-miR-151a Target Sites, and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    240516-plasmid-map-sequence-id-477188

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  15. pEJS2787 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-221 CMV-BFP

    Plasmid
    #240517
    Purpose
    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.
    Depositor
    Erik Sontheimer
    Publication
    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10
    Insert
    AcrIIC3
    Tags
    FLAG, hsa-miR-221 Target Sites, and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    240517-plasmid-map-sequence-id-477189

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  18. pEJS1386 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-107 CMV-BFP

    Plasmid
    #226115
    Purpose
    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.
    Depositor
    Erik Sontheimer
    Publication
    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10
    Insert
    AcrIIC3
    Tags
    FLAG, hsa-miR-107 Target Sites, and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    226115-plasmid-map-sequence-id-448321

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  19. pEJS1350 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-92a CMV-BFP

    Plasmid
    #226109
    Purpose
    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.
    Depositor
    Erik Sontheimer
    Publication
    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10
    Insert
    AcrIIC3
    Tags
    FLAG, hsa-miR-92a Target Sites, and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    226109-plasmid-map-sequence-id-448313

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  20. pEJS1351 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-378a CMV-BFP

    Plasmid
    #226110
    Purpose
    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.
    Depositor
    Erik Sontheimer
    Publication
    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10
    Insert
    AcrIIC3
    Tags
    FLAG, hsa-miR-378a Target Sites, and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    226110-plasmid-map-sequence-id-448316

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  21. pEJS1353 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-33b CMV-BFP

    Plasmid
    #226112
    Purpose
    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.
    Depositor
    Erik Sontheimer
    Publication
    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10
    Insert
    AcrIIC3
    Tags
    FLAG, hsa-miR-33b Target Sites, and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    226112-plasmid-map-sequence-id-448314

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  24. pCX419

    Plasmid
    #229216
    Purpose
    All-in-one plasmid for labeling of XIST RNA via Csm complex
    Depositor
    Jennifer Doudna
    Publication
    Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Csm1; Csm2, Csm3(RNase mut)-sfGFP; Csm4; Csm5; Cas6; XIST crRNA
    Expression
    Mammalian
    Available since
    Feb. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    229216-plasmid-map-sequence-id-457179

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  27. pCMV-eNme2-C.NR

    Plasmid
    #185672
    Purpose
    Expresses nuclease active eNme2-C.NR in mammalian cells
    Depositor
    David Liu
    Publication
    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eNme2-C.NR nuclease
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    eNme2-C S6P/G33E/A520E/S646R/V696F/R711G/V758I/Y7…
    Available since
    Sept. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    185672-plasmid-map-sequence-id-364078

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  28. DOX_SAM_CRISPR-activation-2A-GFP-AAVS1-targeting-construct

    Plasmid
    #176836
    Purpose
    Targeting construct for intergrating a doxycycline (DOX) inducible SAM CRISPR activation complex -2A-GFP to the AAVS1 locus.
    Depositor
    Rudolf Jaenisch
    Publication
    Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-VP64-2A-MS2-p65-HSF1-2A-GFP
    Use
    Unspecified
    Mutation
    See Depositor Comments Below
    Promoter
    Tet operator (tetracycline inducible expression)
    Available since
    Nov. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    176836-plasmid-map-sequence-id-346773

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  29. pX458-Dual-Guide-Donor-ShuttleOnly

    Plasmid
    #171098
    Purpose
    Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.
    Depositor
    Joseph Brzezinski
    Publication
    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2
    Type
    Empty backbone
    Use
    CRISPR
    Mutation
    XbaI site added upstream of U6 promotor. GtoC mu…
    Available since
    Aug. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    171098-plasmid-map-sequence-id-336112

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  34. TLCV2-DelGFP

    Plasmid
    #133302
    Purpose
    EGFP reporter was removed in the TLCV2 vector so it could be used for inducible gene CRISPR knockout in cell lines with an eGFP reporter.
    Depositor
    Jingshi Shen
    Publication
    Wang et al J Biol Chem. 2019 Dec 27;294(52):19988-1
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    133302-plasmid-map-sequence-id-271497

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  36. pMTL3

    Plasmid
    #127966
    Purpose
    constitutive expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locus
    Depositor
    Martin Kampmann
    Publication
    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AAVS1-CAG-KRAB-dCas9-BFP-KRAB
    Use
    TALEN
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Nov. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    127966-plasmid-map-sequence-id-249160

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  37. pMTL5

    Plasmid
    #127967
    Purpose
    DHFR-degron controlled expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locus
    Depositor
    Martin Kampmann
    Publication
    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AAVS1-CAG-DHFR-KRAB-dCas9-BFP-KRAB
    Use
    TALEN
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Nov. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    127967-plasmid-map-sequence-id-249184

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Showing: 4921 - 4960 of 18759 results