We narrowed to 5,415 results for: PEP

1. pMflET-o4

   Plasmid

   #101314

   Purpose

   Contains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and ereB resistance genes, origin of transfer of RP4 plasmid.

   Depositor

   Sébastien Rodrigue

   Article

   Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

   Inserts

   oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
   ereB resistance gene
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHH0103_GNA15_G-alpha

   Plasmid

   #109782

   Purpose

   Protein expression and purification of GNA15_G-alpha

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   GNA15_G-alpha (GNA15 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHH0103_HNF4G_Hormone-recep

   Plasmid

   #109916

   Purpose

   Protein expression and purification of HNF4G_Hormone-recep

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   HNF4G_Hormone-recep (HNF4G Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CMV-SspB-EGFP-VP16-P2A-FLAG-Gal4DBD-CapN-SsrA-CapC-HA

   Plasmid

   #213536

   Purpose

   Expresses the split transcription factors in mouse liver

   Depositor

   Wenjing Wang

   Article

   Shen et al Nat Methods. 2023 Jan;20(1):112-122. doi

   Insert

   SspB-EGFP-VP16-P2A-FLAG-Gal4DBD-CapN-SsrA-CapC-HA-HA
   

   Use

   AAV

   Tags

   EGFP and HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHH0103_ALDOA_Glycolytic

   Plasmid

   #109854

   Purpose

   Protein expression and purification of ALDOA_Glycolytic

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   ALDOA_Glycolytic (ALDOA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-P1-LoxP-Neo-N-miRFP670-C

   Plasmid

   #159434

   Purpose

   CRISPR knock-in donor construct with fluorescent reporter and removable selectable marker

   Depositor

   Jianhua Xing

   Article

   Chen et al Biol Methods Protoc. 2020 Mar 20;5(1):bp

   Inserts

   Neomycin
   miRFP670
   

   Use

   CRISPR

   Tags

   3' linker pair 1, 5' linker pair 1, C t…

   Expression

   Mutation

   Promoter

   SV40

   Available since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHH0103_PCNA_PCNA

   Plasmid

   #110022

   Purpose

   Protein expression and purification of PCNA_PCNA

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   PCNA_PCNA (PCNA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Src(WT)-SsrA(Y7I)-mCer3

   Plasmid

   #220998

   Purpose

   This is microTag (Y7I mutation in SsrA)-Src with mCerulean3 at C-terminal

   Depositor

   Klaus Hahn

   Article

   Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

   Insert

   Src, SsrA, mCerulean3 (Src Mouse)
   

   Use

   Tags

   mCerulean3

   Expression

   Mammalian

   Mutation

   Y7I in SsrA

   Promoter

   CMV

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSFDuet-1_VNp6-StefinA

   Plasmid

   #182404

   Purpose

   Bacterial expression of Vesicle Nucleating peptide6-stefinA fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp6-stefin (CSTA Human)
   

   Use

   Tags

   Vesicle Nucleating peptide 6 (VNp6)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTU387

    Plasmid

    #178981

    Purpose

    pACYC Duet-1 containing full Type III-E system Candidatus “Scalindua brodae” CRISPR array

    Depositor

    Stan Brouns

    Article

    van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

    Insert

    CRISPR array from Candidatus Scalindua brodae
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHH0103_GNA14_G-alpha

    Plasmid

    #109914

    Purpose

    Protein expression and purification of GNA14_G-alpha

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    GNA14_G-alpha (GNA14 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CBA-DIO-GCaMP6s-P2A-mBeRFP-WPRE-pA

    Plasmid

    #175177

    Purpose

    Cre-dependent GCaMP6s and mBeRFP expression

    Depositor

    Bernardo Sabatini

    Article

    Melzer et al Cell. 2021 Oct 28;184(22):5622-5634.e25.

    Insert

    GCaMP6s
    

    Use

    AAV

    Tags

    P2A-mBeRFP

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAP03-act1p

    Plasmid

    #120233

    Purpose

    Carries promoter cassette containing act1p promoter and apramycin resistance gene that can be amplified with homology sequence for refactoring.

    Depositor

    Bradley Moore

    Article

    Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

    Insert

    promoter cassette containing act1p promoter and apramycin resistance gene
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    aac(3)IV (apramycin resistance gene)

    Promoter

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. binder/tag-WT-FAK-insertionB

    Plasmid

    #190440

    Purpose

    The tag is SsrA (AANDENY). Tag is inserted after D405 of FAK.

    Depositor

    Klaus Hahn

    Article

    Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

    Insert

    FAK (Ptk2 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    SsrA inserted after aa 405

    Promoter

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTU425

    Plasmid

    #178984

    Purpose

    pACYC containing Candidatus “Scalindua brodae” TPR-CHAT H585A C627A (codon-optimized)

    Depositor

    Stan Brouns

    Article

    van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

    Insert

    TPR-CHAT H585A C627A
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. tagSrc(WT)-mCer3 S208F/V224L

    Plasmid

    #179138

    Purpose

    mCerulean3 has S208F/V224L mutations that promote weak dimerization with YPet to optimize oritentation for FRET. tag is SsrA (AANDENY).

    Depositor

    Klaus Hahn

    Article

    Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

    Insert

    Src, SsrA
    

    Use

    Tags

    mCerulean3 (S208F/V224L)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHH0103_EIF4E_IF4E

    Plasmid

    #110010

    Purpose

    Protein expression and purification of EIF4E_IF4E

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    EIF4E_IF4E (EIF4E Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX-PP-Spastin(87-616)DeltaExon4

    Plasmid

    #128794

    Purpose

    Bacteiral expression of Spastin M87 varient without exon 4

    Depositor

    Chris Hill

    Article

    Han et al J Biol Chem. 2019 Nov 25. pii: AC119.009

    Insert

    Spastin (SPAST Human)
    

    Use

    `

    Tags

    GST

    Expression

    Bacterial

    Mutation

    residues 87-616 without exon 4.

    Promoter

    T7

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRSFDuet-1_VNp-StefinA

    Plasmid

    #182403

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-stefinA fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp-stefin (CSTA Human)
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. MBC1-PuroIII

    Plasmid

    #153419

    Purpose

    MSCV backbone with murine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1) for expression of TCR genes

    Depositor

    Maki Nakayama

    Article

    Anderson et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

    Insert

    murine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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