We narrowed to 29,485 results for: REP

1. pFA6a-link-ymTq2-URA3

   Plasmid

   #168057

   Purpose

   Replaces Addgene Plasmid 118458. Yeast tagging vector to create a gene fusion with yeast optimized mTurquoise2 with CaUra3 selection; Contains the linker region at the 5’ end of the FP

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymTurquoise2
   

   Use

   Tags

   ymTurquoise2

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNCS-mRuby3

   Plasmid

   #74234

   Purpose

   Constitutive expression of mRuby3 in bacteria

   Depositor

   Michael Lin

   Article

   Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

   Insert

   mRuby3
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-Apex2-Venus-CAAX

   Plasmid

   #168511

   Purpose

   Expression of Apex2 fused to Venus-CAAX for targeting to plasma membrane. Useful for correlated light-eletron microscopy experiments in neurons.

   Depositor

   Franck Polleux

   Article

   Hirabayashi et al Sci Rep. 2018 Sep 27;8(1):14491. doi: 10

   Insert

   Apex2
   

   Use

   Tags

   Venus-CAAX

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEMT-3P2A

   Plasmid

   #111772

   Purpose

   Quadcistronic construct: Co-express four genes by 3 P2A peptides

   Depositor

   Li Qian

   Article

   Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

   Type

   Empty backbone

   Use

   Cloning intermediate

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMK462 (Hygro-P2A-mAB)

   Plasmid

   #214388

   Purpose

   mAID-BromoTag for N-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

   Insert

   Hygro-P2A-mAID-BromoTag
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. modRNAc0-p53DD

   Plasmid

   #176902

   Purpose

   IVT template plasmid for making p53DD modRNA

   Depositor

   Xiaojun Lian

   Article

   Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

   Insert

   p53DD
   

   Use

   Ivt template plasmid

   Tags

   Expression

   Mutation

   Promoter

   T7 promoter

   Available since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. p.UTA.3.0 Empty

   Plasmid

   #82447

   Purpose

   [Edit] Dual Fluorescent endogenous miRNA sensor. To insert the complementary region of the 3'UTR of RFP.

   Depositor

   Jens Gruber

   Article

   Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

   Type

   Empty backbone

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330A_D10A-1x2

   Plasmid

   #58772

   Purpose

   Expresses Cas9 nickase and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 (D10A) nickase
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D10A nickase-converting mutation

   Promoter

   CBh

   Available since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSC101-GFPmut2-mScarlet-I TAA

   Plasmid

   #208187

   Purpose

   nonsense mistranslation, TAA stop

   Depositor

   Tanel Tenson

   Article

   Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

   Inserts

   GFPmut2
   mScarlet-I
   

   Use

   Reporter

   Tags

   Expression

   Bacterial

   Mutation

   Insertion 6TAA*(stop) 7AGT(Ser)

   Promoter

   Ptet+dnaK P1

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLSSmOrange-mKate2

    Plasmid

    #99868

    Purpose

    Measure FRET between LSSmOrange and mKate2

    Depositor

    Marc Tramier

    Article

    Demeautis et al Sci Rep. 2017 Jan 20;7:41026. doi: 10.10

    Insert

    LSSmOrange
    

    Use

    Tags

    mKate2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. p5155

    Plasmid

    #175391

    Purpose

    Low Copy Number DNA Donor Vector for Bxb1 Integrase-mediated Cassette Exchange (for inserts >15kb) into dual site mouse strains (Bxb-GT/GA)

    Depositor

    Michael Wiles

    Article

    Low et al Sci Rep. 2022 Mar 31;12(1):5424. doi: 10

    Type

    Empty backbone

    Use

    Mouse Targeting; Cloning vector, to be used to de…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMK445 (BSD-P2A-BromoTag)

    Plasmid

    #214375

    Purpose

    BromoTag for N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    BSD-P2A-BromoTag
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDest-635

    Plasmid

    #159582

    Purpose

    Gateway Destination vector for protein expression in baculovirus

    Depositor

    Dominic Esposito

    Article

    Yan et al Cell Rep. 2020 Jul 21;32(3):107909. doi:

    Type

    Empty backbone

    Use

    Tags

    His6

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDB179-LCC

    Plasmid

    #218686

    Purpose

    Encodes codon optimized LCC enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    LCC
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. env8 reverse J1/13

    Plasmid

    #99831

    Purpose

    GFPuv reporter that places expression of a fluorescent protein reporter (GFPuv) under control of the env8HyCbl riboswitch.

    Depositor

    Robert Batey

    Article

    Polaski et al Cell Rep. 2016 May 3;15(5):1100-10. doi:

    Insert

    env8 reverse J1/13
    

    Use

    Tags

    GFPuv

    Expression

    Bacterial

    Mutation

    J1/13 sequence is reversed

    Promoter

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTXTL-T7max-FLuc

    Plasmid

    #188311

    Purpose

    Expression of Firefly luciferase via a T7 promoter in TXTL

    Depositor

    Kate Adamala

    Article

    Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

    Insert

    Firefly luciferase
    

    Use

    Luciferase and Synthetic Biology

    Tags

    8x His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7Max

    Available since

    Aug. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pH-tdGFP

    Plasmid

    #74322

    Purpose

    pH stable tandem dimeric sfGFP variant (N149Y,Q204H) in pFA6a-URA3MX. Codon optimized for S. cerevisiae

    Depositor

    Joerg Stelling

    Article

    Roberts et al Sci Rep. 2016 Jun 21;6:28166. doi: 10.10

    Insert

    pH-tdGFP
    

    Use

    Yeast tagging

    Tags

    none

    Expression

    Yeast

    Mutation

    N149Y, Q204H

    Promoter

    Available since

    May 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGWB508-cTP-sfGFP

    Plasmid

    #213863

    Purpose

    Binary vector for the expression of sfGFP flanked with an N-terminal plastid transit peptide in plants (for Agrobacterium-mediated genetic transformation and visualization of plastid.)

    Depositor

    Yutaka Kodama

    Article

    Ishikawa et al Plant Cell Rep. 2023 Mar;42(3):599-607.

    Insert

    Plastid transit peptide fused sfGFP
    

    Use

    Tags

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT2-cryR;cldn5bP1Egfp

    Plasmid

    #90158

    Purpose

    contains cldn5b specific gene promoter driving expression of eGFP

    Depositor

    Nathan Lawson

    Article

    Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

    Insert

    cldn5bP1Egfp (cldn5b Zebrafish)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mNeonGreen-2A-mTurquoise2

    Plasmid

    #98885

    Purpose

    Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET

    Depositor

    Dorus Gadella

    Article

    Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

    Insert

    mNeonGreen-2A-mTurquoise2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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