We narrowed to 4,337 results for: U6 gRNA

1. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide1

   Plasmid

   #118158

   Purpose

   CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the EGFP CDS

   Depositor

   Jorge Ferrer

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a core and U6

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR BFP-guide1

   Plasmid

   #118157

   Purpose

   CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the BFP CDS

   Depositor

   Jorge Ferrer

   Insert

   KRAB-dCas9-P2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a core and U6

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide2

   Plasmid

   #118159

   Purpose

   CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA2 against the EGFP CDS

   Depositor

   Jorge Ferrer

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a core and U6

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide3

   Plasmid

   #118160

   Purpose

   CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA3 against the EGFP CDS

   Depositor

   Jorge Ferrer

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a core and U6

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Circular 200,100 (mPCSK9)

   Plasmid

   #170122

   Purpose

   AAV vector carrying 2 copies of a guide RNA targeting the mouse PCSK9 mRNA, expressed from human and mouse U6 promoters

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

   Insert

   Circular 200,100 guide RNA (Pcsk9 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6 and mouse U6

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Circular 200,100 (mIDUA)

   Plasmid

   #170123

   Purpose

   AAV vector carrying 2 copies of a guide RNA targeting the mouse IDUA mRNA, expressed from human and mouse U6 promoters

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

   Insert

   Circular 200,100 guide RNA (Idua Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6 and mouse U6

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pU6-sgGAL4-1

   Plasmid

   #46915

   Purpose

   Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GAL4 UAS promoter

   Depositor

   Stanley Qi, Jonathan Weissman

   Article

   Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

   Inserts

   sgGAL4-1
   Puromycin resistance and mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pU6-sgGAL4-4

   Plasmid

   #46916

   Purpose

   Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GAL4 UAS promoter (negative control)

   Depositor

   Stanley Qi, Jonathan Weissman

   Article

   Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

   Inserts

   sgGAL4-4
   Puromycin resistance and mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TLCV2-LoxP

   Plasmid

   #127098

   Purpose

   LentiCRISPR v2 was modified into an all-in-one dox inducible system.

   Depositor

   Adam Karpf

   Insert

   Cas9-2A-eGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLKO.1-blast shGFP

    Plasmid

    #110419

    Purpose

    shGFP control, silence GFP gene, blasticidin selection.

    Depositor

    Sandra Martha Gomes Dias

    Insert

    GFP
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (RNA PolIII)

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pXPR_016

    Plasmid

    #107143

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Hygro resistance from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    David Root

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pU6:3-tevopreq1

    Plasmid

    #190909

    Purpose

    Expression of single epegRNA under control of Drosophila U6:3 promoter. Can be used to generate transgenic flies with vermillion+ selection.

    Depositor

    Norbert Perrimon

    Insert

    Empty Backbone
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSc2

    Plasmid

    #80437

    Purpose

    Expresses eGFP along with an U6 promoter driven gRNA which cleaves the plasmid in-cell

    Depositor

    Ervin Welker

    Article

    Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare

    Inserts

    sp Cas9 gRNA
    eGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    Sept. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. BPK2660

    Plasmid

    #70709

    Purpose

    Human expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pChimera

    Plasmid

    #61476

    Purpose

    Vector for conventional cloning that contains AtU6-26 promoter and sgRNA backbone

    Depositor

    Holger Puchta

    Article

    Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

    Insert

    U6-26:sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    U6-26

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pB-puro-mU6-CNPshRNA2

    Plasmid

    #126612

    Purpose

    Expresses shRNA against human CNP under mouse U6 promoter

    Depositor

    Xiaozhong Wang

    Article

    Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

    Insert

    CNP ShRNA-2
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Mouse U6 promoter

    Available since

    June 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKJ247

    Plasmid

    #228714

    Purpose

    Mammalian expression of U6 epPsaCas12f RNF2 targeting guide

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Jiang et al Science 0, eadr6006

    Insert

    u6-RNF2 targeting guide
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pB-puro-mU6-RtcAshRNA1

    Plasmid

    #126613

    Purpose

    Expresses shRNA against human RtcA under mouse U6 promoter

    Depositor

    Xiaozhong Wang

    Article

    Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

    Insert

    RtcA shRNA-1
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    mouse U6 promoter

    Available since

    June 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pB-puro-mU6-RtcAshRNA2

    Plasmid

    #126614

    Purpose

    Expresses shRNA against human RtcA under mouse U6 promoter

    Depositor

    Xiaozhong Wang

    Article

    Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

    Insert

    RtcA shRNA-2
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Mouse U6 promoter

    Available since

    June 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLV(shRNA)-Puro-6 CCEPR Vb2

    Plasmid

    #108278

    Purpose

    shRNA for CCEPR lncRNA

    Depositor

    Karl Munger

    Article

    Sharma et al Virology. 2018 Feb 7;518:8-13. doi: 10.1

    Insert

    CCEPR (CCEPR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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