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We narrowed to 24,925 results for: crispr

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Showing: 2461 - 2480 of 24925 results

  1. pSL5069 (pDonor(AAVS1),CRISPR_Pse)

    Plasmid
    #200904
    Purpose
    Encodes a mini-transposon derived from Pseudoalteromonas Tn7016 with a splice acceptor-T2A-PuroR-T2A-eGFP cargo, and a CRISPR RNA under a U6 promoter. Total transposon size = 2,024 bp.
    Depositor
    Samuel H. Sternberg
    Publication
    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Mini Tn7016 (AAVS1 NGS), PseCRISPR (tSL425)
    Expression
    Mammalian
    Available since
    May 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    200904-plasmid-map-sequence-id-393155

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  2. pCRISPRi

    Plasmid
    #199808
    Purpose
    vector for constitutive CRISPRi-mediated gene knockdown in S. aureus
    Depositor
    Steve Salipante
    Publication
    Long et al PLoS Pathog. 2024 Jul 11;20(7):e1012394.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    dCas9
    sgRNA with GFP cassette
    Use
    CRISPR
    Promoter
    P23 and PRAB17 (sgRNA) and SarAP1 (GFP)
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    199808-plasmid-map-sequence-id-403016

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  10. pEJS2702-CMV-iNme2-ABE8e-nt

    Plasmid
    #228723
    Purpose
    Expresses N-terminal ABE8e-iNme2 nCas9 (D16A) base editor in mammalian cells
    Depositor
    Erik Sontheimer
    Publication
    Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    N-terminal ABE8e with iNme2 nCas9 (D16A)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Nme2-Cas9 D16A D844G E868K K870R D873A D911G K929…
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    228723-plasmid-map-sequence-id-454010

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  11. pEJS2703-CMV-iNme2Smu-ABE8e-nt

    Plasmid
    #228724
    Purpose
    Expresses N-terminal ABE8e-iNme2Smu nCas9 (D16A) base editor in mammalian cells
    Depositor
    Erik Sontheimer
    Publication
    Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    N-terminal ABE8e with iNme2Smu nCas9 (D16A)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Nme2-Cas9, Smu PID, D16A D844G E868K K870R D873A …
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    228724-plasmid-map-sequence-id-454004

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  18. pEJS2711-CMV-Nme2-ABE8e-i1-L10-D56R

    Plasmid
    #228732
    Purpose
    Expresses domain inlaid Nme2 nCas9 (D16A)-i1-ABE8e base editor with linker-10 and D56R mutation in mammalian cells
    Depositor
    Erik Sontheimer
    Publication
    Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Domain inlaid Nme2 nCas9 (D16A)-i1-L10-D56R
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Nme2-Cas9 D16A D56R
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    228732-plasmid-map-sequence-id-453998

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  19. pEJS2710-CMV-Nme2-ABE8e-i1-L10-E932R

    Plasmid
    #228731
    Purpose
    Expresses domain inlaid Nme2 nCas9 (D16A)-i1-ABE8e base editor with linker-10 and E932R mutation in mammalian cells
    Depositor
    Erik Sontheimer
    Publication
    Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Domain inlaid Nme2 nCas9 (D16A)-i1-L10-E932R
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Nme2-Cas9 D16A E932R
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    228731-plasmid-map-sequence-id-453993

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  20. HeFm2SpCas9

    Plasmid
    #92111
    Purpose
    Expression plasmid for human codon-optimized increased fidelity HeFm2SpCas9 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    “Highly enhanced Fidelity” mut2 SpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, K848A, Q926A, R1060A
    Promoter
    Cbh
    Available since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92111-plasmid-map-sequence-id-197616

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Showing: 2461 - 2480 of 24925 results