We narrowed to 7,126 results for: ef1a
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Plasmid#201560PurposeEcdysone-inducible vector with SB ITRs for expressing FLAG- EGFP-DD (WPRE in 3' UTR) and DBEcR (3xmyc-tagged); blasticidin resistance.DepositorInsertDBEcR and EGFP
UseUnspecified; Sleeping beauty transposonTagsDBEcR-myc, FLAG-EGFP, and FLAG-EGFP-DDExpressionMammalianMutationPromoterEF1a and pIND(SP1)Available sinceJuly 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR RPS27A K107/113R-3xFlag_IRES-GFP
Plasmid#198392PurposeRPS27A K107/113R (ubiquitin/ribosomal protein eS31 fusion) expressionDepositorInsertRPS27A (RPS27A Human)
UseLentiviralTags3xFlagExpressionMammalianMutationubiquitination site double mutant (K107/113R)PromoterEF1AAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWPXL-Hes1
Plasmid#36983DepositorInsertHes1 (Hes1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
AA065
Plasmid#215965PurposeFragmid fragment: (Pol 2 promoter) human EF1a promoterDepositorHas ServiceCloning Grade DNAInsertEF1a_v1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pONSY-CoNMM:mCherry
Plasmid#111878PurposeThis plasmid expresses mCherry fluorescent protein fused to an N-Myristoylation motif (NMM) from the endogenous Src2 gene (CAOG_06360). It can be used to visualize the membrane and filopodia.DepositorInsertCapsaspora N-Myristoylation motif (CoNMM) from the Src2 gene fused to mCherry
UseCapsaspora owczarzakiTagsmCherryExpressionMutationPromoterElongation Factor 1 alpha (EF1a) from CapsasporaAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSH231-EF1-BLST-Cas9-VPR
Plasmid#115147PurposeSafe harbor site 231 knock-in vector with BlastR-Cas9-VPR expression cassetteDepositorInsertBlastR-P2A-Cas9-VPR
UseCRISPRTagsSV40NLSExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKS7107
Plasmid#89051PurposeExpresses Nm crRNA, Nm tracrRNA and human codon-optimized NmCas9DepositorInsertsNm crRNA
Nm tracrRNA
hNmCas9
UseCRISPRTagsHA tag, NLS, and SV40 NLSExpressionMutationPromoterEF1a and human U6Available sinceApril 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEF1α-M2rtTA-T2A-Puro
Plasmid#169175PurposepiggyBac co-transfection transposon containing M2rtTA promoter linked with Puromycin resistance gene, through T2A with GSG linker, subcloned between ends of two ITRs. Pairs with #169174DepositorInsertM2rtTA-T2A-Puro
UsePiggybac transposonTagsExpressionMammalianMutationSee Depositor Comments BelowPromoterEF1aAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ10530
Plasmid#239266PurposeExpresses NES-dCas13b-ABI for CRISPR-TO perturbation in the cytoplasm of cell linesDepositorInsertNES-dPspCas13b-EGFP-ABI
UseCRISPR and LentiviralTagsFlag tag and V5 tagExpressionMammalianMutationH133A and H1058A in PspCas13bPromoterEF1aAvailable sinceSept. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSLQ10531
Plasmid#239267PurposeExpresses NLS-dCas13b-ABI for CRISPR-TO perturbation in the nucleus of cell linesDepositorInsertNLS-dPspCas13b-EGFP-ABI
UseCRISPR and LentiviralTagsFlag tag and V5 tagExpressionMammalianMutationH133A and H1058A in PspCas13bPromoterEF1aAvailable sinceSept. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-mCherry-Parkin
Plasmid#237397PurposeLentivirus plasmid for the stable expression of mCherry-Parkin in mammalian cellsDepositorInsertPRKN (PRKN Human)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterEF1AAvailable sinceAug. 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-HyperdLbCas12a-NLS-3xFlag
Plasmid#236386PurposeOverexpression of HyperdLbCas12a in human cellsDepositorInsertHyperdLbCas12a
UseCRISPR and LentiviralTagsExpressionMutationD156R, D235R, E292R, D350RPromoterAvailable sinceJuly 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-HyperdFnCas12a-EGFP-NLS-3xFlag
Plasmid#236376PurposeOverexpression of HyperdFnCas12a in human cellsDepositorInsertHyperdFnCas12a
UseCRISPR and LentiviralTagsExpressionMutationE184R, T279R, D340R, D409RPromoterAvailable sinceJuly 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-HyperdLbCas12a-StayGold-NLS-3xFlag
Plasmid#236368PurposeOverexpression of HyperdLbCas12a in human cellsDepositorInsertHyperdLbCas12a
UseCRISPR and LentiviralTagsExpressionMutationD156R, D235R, E292R, D350RPromoterAvailable sinceJuly 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dFnCas12a-EGFP-NLS-3xFlag
Plasmid#236375PurposeOverexpression of dFnCas12a in human cellsDepositorInsertdFnCas12a
UseCRISPR and LentiviralTagsExpressionMutationWTPromoterAvailable sinceJuly 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-endAsCas12a-EGFP-NLS-3xFlag
Plasmid#236363PurposeOverexpression of endAsCas12a in human cellsDepositorInsertendAsCas12a
UseCRISPR and LentiviralTagsExpressionMutationE174R, S542R, K548RPromoterAvailable sinceJuly 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-HyperdAsCas12a-EGFP-NLS-3xFlag
Plasmid#236362PurposeOverexpression of HyperdAsCas12a in human cellsDepositorInsertHyperdAsCas12a
UseCRISPR and LentiviralTagsExpressionMutationE174R, D256R, E321R, E371RPromoterAvailable sinceJuly 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dAsCas12a-EGFP-NLS-3xFlag
Plasmid#236361PurposeOverexpression of dAsCas12a in human cellsDepositorInsertdAsCas12a
UseCRISPR and LentiviralTagsExpressionMutationWTPromoterAvailable sinceJuly 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1_-DIO-Varnam1-WPRE
Plasmid#240054PurposeCre dependent Red fluorescent voltage indicator for mammalian expressionDepositorInsertVARNAM1
UseAAVTagsExpressionMutationPromoterEf1aAvailable sinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
lentiGuideFE-mSG-Puro
Plasmid#226522PurposeCas9 guide RNA scaffold with the F+E scaffold modification with mStayGold-P2A-PuroDepositorInserthU6_SpCas9gRNAF+E_EF1a_mStayGold_P2A_Puro
UseCRISPR and LentiviralTagsmStayGoldExpressionMammalianMutationPromoterU6,EF1aAvailable sinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only