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MSCV-IRES-Tomato MEF2C

Plasmid

#89715

Purpose

Retroviral gene expression vector for MEF2C expression

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Insert

MEF2C (MEF2C Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 1, 2017

Availability

Academic Institutions and Nonprofits only

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retro-gfpIkkb-puro vector

Plasmid

#58251

Purpose

Retroviral expression vector encoding bicistronic EGFP-IKKbeta and Puromycin cassette

Depositor

Georgios Stathopoulos

Article

Giannou et al J Clin Invest. 2015 Jun;125(6):2317-34.

Insert

Ikkβ (Ikbkb Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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pSIN-TRE-H2BeGFP-rtTA2

Plasmid

#165494

Purpose

Doxycycline-inducible all-in-one lentivirus vector to express H2BeGFP fusion protein. System for labelling slow-cycling cells in vivo.

Depositor

Hector G. Palmer

Article

Puig et al J Clin Invest. 2018 Aug 31;128(9):3887-3

Insert

Human histone H2B (H2BC21 Human)

Use

Lentiviral

Tags

EGFP

Mutation

-

Promoter

TetO-CMV

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pINDUCER20 MEF2C

Plasmid

#89717

Purpose

Lentiviral gene expression vector for doxycycline inducible MEF2C expression

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Insert

MEF2C (MEF2C Human)

Use

Lentiviral

Expression

Mammalian

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-VSIR-Fc(DAPA)-AviTag-6xHis

Plasmid

#156555

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

VSIR (C10orf54 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pINDUCER20 MEF2C S222A

Plasmid

#89718

Purpose

Lentiviral gene expression vector for doxycycline inducible MEF2C-S222A expression

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Insert

MEF2C (MEF2C Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed serine 222 to alanine

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-IRES-Tomato MEF2C S222A

Plasmid

#89716

Purpose

Retroviral gene expression vector for MEF2C-S222A expression

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Insert

MEF2C (MEF2C Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 1, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-IRES_Tomato MEF2C S222D

Plasmid

#107231

Purpose

hMEF2C phosphomimetic obtained from Quikchange Lightning Mutagenesis

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Insert

MEF2C (MEF2C Human)

Use

Retroviral

Expression

Mammalian

Mutation

S222D

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pROM-POU3F2p-mCherry-Neo

Plasmid

#153321

Purpose

Expresses mCherry from the POU3F2 promoter

Depositor

Robert Judson-Torres

Article

Zhang et al Cell Rep. 2025 Dec 23;44(12):116675. doi

Insert

POU3F2 Promoter (POU3F2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

N/A

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-PB2_Lock1-I436C+S993C

Plasmid

#182879

Purpose

Lentivirus for expression of Plexin-B2 locked ring mutant

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2025 Jan 2;16(1):272. doi: 1

Insert

hPLXNB2 (PLXNB2 Human)

Use

Lentiviral

Mutation

changed Isoleucine 436 to Cysteine and Serine 993…

Available Since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-PB2_Lock2-I436C+T1051C

Plasmid

#182880

Purpose

Lentivirus for expression of Plexin-B2 locked ring mutant

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2025 Jan 2;16(1):272. doi: 1

Insert

hPLXNB2 (PLXNB2 Human)

Use

Lentiviral

Mutation

changed Isoleucine 436 to Cysteine and Threonine …

Available Since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLT3REVIR_MARK3 2573

Plasmid

#107233

Purpose

shRNA 2573. Do not use Evos or Acurri on these cells. Assess by FACS specifically with Venus and DsRed lazers. Tet-ON-all-in-one

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Insert

MARK3 shRNA (22 nt) (MARK3 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pLT3REVIR_MARK3 1122

Plasmid

#107232

Purpose

shRNA 1122. Do not use Evos or Acurri on these cells. Assess by FACS specifically with Venus and DsRed lazers. Tet-ON-all-in-one

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Insert

MARK3 shRNA (22 nt) (MARK3 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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P3-dCas9-Tet1-GFP-Puro

Plasmid

#190728

Purpose

Expression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)

Depositor

Maja Jagodic

Article

Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:

Insert

dCas9-tet1

Use

CRISPR

Tags

3XFLAG and SV40 NLS

Expression

Mammalian

Mutation

Changed Pro434 codon from CCG to CCC and Gly468 f…

Promoter

pCAG

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4-eIF3B

Plasmid

#133308

Purpose

Expression of luciferase driven by eIF3B promoter region

Depositor

Josep Domingo-Domenech

Article

Santasusagna et al Cancer Discov. 2023 Sep 7. doi: 10.1158/

Insert

eIF3B promoter (EIF3B Human)

Use

Luciferase

Expression

Mammalian

Promoter

eIF3B

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pET-30a (+)_CDK2-7 chimera (I10L,T14Q,V64I,K88E,K89V,D92K,Q131N)

Plasmid

#217014

Purpose

Expression of CDK2-7 chimera in E.coli

Depositor

Manjeet Mukherjee

Article

Mukherjee et al Structure. 2024 Jun 9:S0969-2126(24)0018

Insert

CDK2-7 chimera (I10L,T14Q,V64I,K88E,K89V,D92K,Q131N) (CDK7, CDK2 Human)

Tags

C3 protease cleavable GST tag

Expression

Bacterial

Mutation

CDK2 (I10L,T14Q,V64I,K88E,K89V,D92K,Q131N)

Available Since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4-eIF3B MBE3 mutant

Plasmid

#133310

Purpose

Expression of luciferase driven by eIF3B promoter region mutated at the E-box binding motif 3

Depositor

Josep Domingo-Domenech

Article

Santasusagna et al Cancer Discov. 2023 Sep 7. doi: 10.1158/

Insert

eIF3B promoter MBE3 mutant (EIF3B Human)

Use

Luciferase

Expression

Mammalian

Mutation

ccacgtgacc changed to cAaAAAAacc

Promoter

eIF3B

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pGL4-eIF3B MBE2 mutant

Plasmid

#133309

Purpose

Expression of luciferase driven by eIF3B promoter region mutated at the E-box binding motif 2

Depositor

Josep Domingo-Domenech

Article

Santasusagna et al Cancer Discov. 2023 Sep 7. doi: 10.1158/

Insert

eIF3B promoter MBE2 mutant (EIF3B Human)

Use

Luciferase

Expression

Mammalian

Mutation

gccacatgcacc changed to gcAaAaAAcacc

Promoter

eIF3B

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pLV V5-TbID-emerin

Plasmid

#175099

Purpose

Lentiviral expression of V5-TbID-tagged mouse Emd

Depositor

Larry Gerace

Article

Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

Insert

Emerin (Emd Mouse)

Use

Lentiviral

Tags

V5-TbID

Expression

Mammalian

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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bActin-RSG-BiSKi

Plasmid

#26437

Depositor

Didier Stainier

Insert

bActin promoter

Use

4726

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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