We narrowed to 18,057 results for: puro

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pLentiRNACRISPR_006 - hU6-DR_BsmBI-EFS-RfxCas13d-NES-2A-Puro-WPRE

Plasmid

#138148

Purpose

Expresses RfxCas13d in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

Depositor

Neville Sanjana

Article

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

CasRx

Use

Lentiviral

Tags

NES

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-SARS-CoV-2-nsp5-2xStrep-IRES-Puro

Plasmid

#141370

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp5 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGL3- sgAAVS1.5-CAG-Cas9-T2A-mCherry-P2A-Puro

Plasmid

#216246

Purpose

Express Cas9 with CAG promoter to improve the expression in hES cells and a highly efficient sgAAVS1.5 for AAVS1 integration

Depositor

Elina Ikonen

Article

Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

Insert

Cas9 and sgAAVS1.5

Use

CRISPR; Human safe harbor locus (a avs1) site-spe…

Expression

Mammalian

Promoter

CAG

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-NLS-dPspCas13b-3xsfGFP-NLS

Plasmid

#132404

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Expression

Mammalian

Mutation

H133A; H1059A

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-SARS-CoV-2-nsp8-2xStrep-IRES-Puro

Plasmid

#141374

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp8 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti CMV TRE3G Puro FLAG-DD-ER-mCherry-TRF1-FokI WT

Plasmid

#207832

Purpose

telomere specific nuclease to create double-strand breaks at telomeres

Depositor

Roger Greenberg

Article

Dilley et al Nature. 2016 Nov 3;539(7627):54-58. doi:

Insert

TRF1 (TERF1 Human)

Use

Lentiviral

Tags

Flag, DD, ER, mCherry and FokI

Expression

Mammalian

Promoter

TRE3G

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-p53RE(CDKN1A)-CMVmin-mScarlet-I-NLSx3-AU1-PGKpuro

Plasmid

#241845

Purpose

Expresses p53 transcription fluorescence reporter in mammalian cells.

Depositor

Kazuhiro Aoki

Article

Tsuruoka et al Cell Struct Funct. 2025 Jun 12. doi: 10.

Insert

CDKN1A promoter (CDKN1A Human)

Tags

mScarlet-I-3xNLS

Expression

Mammalian

Mutation

CDKN1A promoter (1015 bp) + CMV minimal promoter …

Promoter

n/a

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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tet-TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA

Plasmid

#221235

Purpose

Tet-inducible lentiviral HACE Editor as a fusion of TadA-8e adenosine deaminase and an optimized PcrA M6 helicase.

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA

Use

Lentiviral

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-Ins-TRE3Gp-dCas9-Suntag-VP64-EF1Ap-Puro

Plasmid

#183409

Purpose

piggyBAC-based doxycycline-inducible dCas9-5xSuntag-VP64 CRISPR activation plasmid for enhancer and promoter activation in mammalian cells

Depositor

Azim Surani

Article

Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

Insert

dCas9-5xGCN5-P2A-scFV-sfGFP-VP64-GB1

Use

CRISPR and Synthetic Biology; Piggybac

Expression

Mammalian

Promoter

TRE3G

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-SARS-CoV-2-nsp12-2xStrep-IRES-Puro

Plasmid

#141378

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp12 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-EF1alpha-SARS-CoV-2-nsp7-2xStrep-IRES-Puro

Plasmid

#141373

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp7 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pRC49-U6-DR-crRNA-BsmbI(x2)-6T; EFS-Puro-2A-Fluc-2A-EGFP_NLS-WPRE

Plasmid

#123363

Purpose

Lentiviral vector with empty U6 cassette containing LbCpf1 direct repeat and U6 terminator, with constitutive expression of puromycin resistance, Firefly luciferase, and nuclear EGFP.

Depositor

Sidi Chen

Article

Chow et al Nat Methods. 2019 May;16(5):405-408. doi

Insert

EGFP-NLS

Use

CRISPR, Lentiviral, and Luciferase

Expression

Mammalian

Promoter

EFS

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pSLQ8454 pHR-hU6-LbCpf1 crRNA BB-EF1a-Puro-WPRE

Plasmid

#140087

Purpose

Backbone for cloning U6 driven LbCpf1 (Cas12a) crRNA, with EF1a driven puromycin resistance

Depositor

Stanley Qi

Article

Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

Insert

LbCpf1 (LbCas12a) crRNA backbone

Use

Lentiviral

Tags

EF1a-Puro

Expression

Mammalian

Promoter

U6

Available Since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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MSCV-(mcs) human CSF1 full length-IRES-Puro

Plasmid

#86797

Purpose

retroviral expression of CSF-1

Depositor

Martine Roussel, Charles Sherr

Article

Roussel et al Nature. 1987 Feb 5-11;325(6104):549-52.

Insert

CSF-1 (CSF1 Human)

Use

Retroviral

Tags

IRES-Puro

Expression

Mammalian

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-SARS-CoV-2-nsp1-2xStrep-IRES-Puro

Plasmid

#141367

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp1 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

Plasmid

#42229

Purpose

This plasmid separately encodes a human codon-optimized SpCas9, a tracrRNA and customizable crRNA.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Available Since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-SARS-CoV-2-nsp13-2xStrep-IRES-Puro

Plasmid

#141379

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp13 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pHAGE-IRES-puro-NLS-dLwaCas13a-EGFP-NLS-3xFlag

Plasmid

#132407

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dLwaCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

R474A; H479A; R1046A; R1051A

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pLY092b-lenti-EFS-BCMA-BBz-T2APRODH2-T2APuro-WPRE

Plasmid

#192193

Purpose

Lenti-BCMA-CAR-PRODH2

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

Lenti-BCMA-CAR-PRODH2 (TNFRSF17 Synthetic)

Use

Lentiviral; Mammalian expression

Mutation

NA

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-HyperdLbCas12a-StayGold-NLS-3xFlag

Plasmid

#236368

Purpose

Overexpression of HyperdLbCas12a in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

HyperdLbCas12a

Use

CRISPR and Lentiviral

Mutation

D156R, D235R, E292R, D350R

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pSLQ4419 pHR (hU6-BsmBI-BsmBI-EFS-PuroR-WPRE)

Plasmid

#214875

Purpose

Empty lentiviral backbone for guide array cloning

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-SARS-CoV-2-nsp2-2xStrep-IRES-Puro

Plasmid

#141368

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp2 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pLVX-EF1alpha-SARS-CoV-2-orf7a-2xStrep-IRES-Puro

Plasmid

#141388

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-orf7a (ORF7a SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pLVX-EF1alpha-SARS-CoV-2-orf3a-2xStrep-IRES-Puro

Plasmid

#141383

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-orf3a (ORF3a SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pHAGE-IRES-puro-NLS-dRfxCas13d-EGFP-NLS-3xFlag

Plasmid

#132411

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dRfxCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

R239A; H244A; R858A; H863A

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-SARS-CoV-2-orf8-2xStrep-IRES-Puro

Plasmid

#141390

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-orf8 (ORF8 SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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tre-stblHIF: pLenti-TRE-stblHIF-PGK-rtTAA-2A-Puro

Plasmid

#177203

Purpose

encoding stblHIF under inducible promoter

Depositor

Markus Covert

Article

Kudo et al bioRxiv (2022)

Insert

stblHIF

Use

Lentiviral

Expression

Mammalian

Mutation

P402A/P577G/N813A

Promoter

TRE

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-protein1-IRES-protein2-IRESatt-EGFP-2A-PuroR-SMAR-g5

Plasmid

#208377

Purpose

Episomal plasmid for expression of 2 proteins with puromycin selection and EGFP monitoring

Depositor

Daniel Hawiger

Article

Fallahee et al Antibodies (Basel). 2024 Mar 11;13(1):18

Type

Empty backbone

Expression

Mammalian

Promoter

human CMV

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPspCas13b-EGFP-NLS-3xFlag

Plasmid

#132397

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H133A; H1058A

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-G3635A-L17-FZY2-S100N-UGI-Puro

Plasmid

#209642

Purpose

To install m.G3635 mutation on mtDNA

Depositor

Bin Shen

Article

Sun et al Nat Commun. 2023 Oct 19;14(1):6625. doi:

Insert

L17-FZY2-S100N

Expression

Mammalian

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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