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Showing: 4961 - 5000 of 8780 results

  1. (829) SB Tet-ON dCasphi-[myc-ZIM3-HA] U6-gStop

    Plasmid
    #195622
    Purpose
    Sleeping Beauty Tet-ON plasmid for induced expression of dCasphi-ZIM3 CRISPRi construct
    Depositor
    Tonia Rex
    Insert
    dCasphi
    Tags
    C-Myc (NLS), HA tag, and ZIM3
    Expression
    Mammalian
    Mutation
    P749A and P753A
    Promoter
    Tight TRE promoter; Tet-ON (doxycycline)
    Available Since
    July 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    195622-plasmid-map-sequence-id-385115

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  2. Human Coxsackievirus A16 strain G10 2A protease

    Plasmid
    #228632
    Purpose
    Bacterial expression of the Coxsackievirus A16 (G-10) 2A protease this construct can be used for biochemical assay and to obtain crystals in the space group C1 2 1(PDB 8POA)
    Depositor
    ASAP Discovery, Frank von Delft
    Insert
    Enterovirus Coxsackievirus A16 2A protease (HEVAgp1 Coxsackievirus 16)
    Tags
    His-SUMO
    Expression
    Bacterial
    Available Since
    Feb. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228632-plasmid-map-sequence-id-461401

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  3. pMX-Flag-GFP_ENPP5

    Plasmid
    #227956
    Purpose
    Control plasmid. Important note: Construct with N-terminally tagged GFP which might mask the signal sequence for proper localization of the protein
    Depositor
    Alessandro Ori
    Insert
    ENPP5 (ENPP5 Human)
    Use
    Lentiviral
    Tags
    3xFLAG-EGFP
    Expression
    Mammalian
    Available Since
    Feb. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    227956-plasmid-map-sequence-id-461327

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  15. pCASCADE-fabI-gltA1-gltA2-udhA-zwf

    Plasmid
    #87148
    Purpose
    fabI-gltA1-gltA2-udhA-zwf targeting guide RNA E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    fabI-gltA1-gltA2-udhA-zwf guide array
    Expression
    Bacterial
    Promoter
    E . coli ugpB gene promoter, low phosphate induct…
    Available Since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87148-plasmid-map-sequence-id-175306

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  16. pCASCADE-fabI-gltA1-gltA2-udhA-gapAP1

    Plasmid
    #87154
    Purpose
    fabI-gltA1-gltA2-udhA-gapAP1 targeting guide RNA E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    fabI-gltA1-gltA2-udhA-GapAP1 guide array
    Expression
    Bacterial
    Promoter
    E . coli ugpB gene promoter, low phosphate induct…
    Available Since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87154-plasmid-map-sequence-id-174942

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  17. ReaLiSM A1-43-GPIba+2cys

    Plasmid
    #59577
    Purpose
    express VWF A1-GPIba ReaLiSM construct for single molecule study
    Depositor
    Timothy Springer
    Insert
    VWF A1-linker-GPIba (VWF Human)
    Tags
    6His
    Expression
    Mammalian
    Promoter
    chick beta-actin
    Available Since
    Nov. 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  18. pET29a-human histone 4-in-One

    Plasmid
    #223237
    Purpose
    Polycistronic bacterial coexpression construct for human His-Sumo-H2A, H2B, H3, and Dual-StrepII-H4
    Depositor
    Weixing Zhao
    Inserts
    His6-VSVG-Histone H2A
    Histone H2B
    Histone H3
    Histone H4-TEV-His6-Dual StrepII
    Tags
    Dual StrepII, His6, TEV protease site, and VSVG
    Expression
    Bacterial
    Mutation
    A103G
    Promoter
    T7
    Available Since
    Aug. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    223237-plasmid-map-sequence-id-443806

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  20. AAVS1-DRGFP

    Plasmid
    #113193
    Purpose
    DR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.
    Depositor
    Adam Karpf
    Insert
    DR-GFP
    Use
    Donor construct with t2a-hygromycin for gene trap…
    Expression
    Mammalian
    Available Since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    113193-plasmid-map-sequence-id-216317

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  36. pPEE16

    Plasmid
    #128360
    Purpose
    Dominant marker in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 1, a small gene-free region. Contains the amdS2 counterselectable marker.
    Depositor
    James Fraser
    Article
    Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324
    Type
    Empty backbone
    Use
    Unspecified
    Promoter
    TEF1
    Available Since
    Oct. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    128360-plasmid-map-sequence-id-254521

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Showing: 4961 - 5000 of 8780 results