We narrowed to 17,082 results for: EMB

1. pAV114

   Plasmid

   #63181

   Purpose

   pRS414 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOPS0253

   Plasmid

   #115505

   Purpose

   Suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing gusA.

   Depositor

   Philip Poole

   Article

   Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

   Insert

   Rlv3841pNifH pOGG082, gusA pOGG083, T-pharma pOGG003 assembled in pOGG026
   

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBTK401

   Plasmid

   #110597

   Purpose

   BTK Type 8 Bacterial origin (broad-host-range) for golden gate assembly

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   mRFP dropout (RSF1010 origin)
   

   Use

   Synthetic Biology

   Available Since

   Feb. 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pLaNGo11-1

   Plasmid

   #126053

   Purpose

   A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell

   Depositor

   Ralf-Bernd Klösgen

   Article

   Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

   Inserts

   chloroplastic FNR transit peptide
   ccdB cassette (BsaI)
   GFP11
   

   Use

   Binary

   Tags

   GFP1-10

   Expression

   Plant

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSCIPpay-BFP reporter

   Plasmid

   #154096

   Purpose

   Self-Cutting and Integrating Payload CRISPR backbone with BFP reporter

   Depositor

   Scott McComb

   Article

   Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

   Insert

   P2A-tagBFP
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMM2-hPMCA4a

   Plasmid

   #47761

   Purpose

   mammalian expression of human PMCA4a

   Depositor

   Emanuel Strehler, John Penniston

   Article

   Enyedi et al J Biol Chem. 1994 Jan 7;269(1):41-3.

   Insert

   PMCA4a (ATP2B4 Human)
   

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Sept. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBTK130

   Plasmid

   #110584

   Purpose

   BTK Type 2 promoter for golden gate assembly

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   T7 promoter with lac operator and RBS
   

   Use

   Synthetic Biology

   Available Since

   Feb. 20, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pBTK550d

   Plasmid

   #110617

   Purpose

   BTK assembled broad-host-range plasmid encoding inducible GFP driven by T7 polymerase

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   GFP
   

   Expression

   Bacterial

   Available Since

   Aug. 10, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pKT3Ts-goldyTALEN

   Plasmid

   #80330

   Purpose

   Updated TALEN vector; compatible with the Golden Gate TALEN assembly protocol. More stable backbone with reduced recombination compared to pT3Ts-goldyTALEN due to the removal of a lacZ sequence

   Depositor

   Jeffrey Essner

   Article

   Welker et al Hum Gene Ther. 2016 Jun;27(6):423-4. doi

   Type

   Empty backbone

   Use

   TALEN

   Expression

   Bacterial

   Promoter

   T3

   Available Since

   Aug. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. L1-1147

    Plasmid

    #13267

    Depositor

    Vance Lemmon

    Article

    Cheng et al J Neurosci. 2005 Jan 12. 25(2):395-403.

    Insert

    L1CAM (L1CAM Human)
    

    Expression

    Bacterial

    Mutation

    Truncated in the juxtamembrane region

    Available Since

    Dec. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

11. NET33 pmRFP-N2 (448)

    Plasmid

    #61979

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

    Insert

    NET33 (SCARA5 Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

12. MAC_C_EPHA8

    Plasmid

    #187761

    Purpose

    MAC-tagged gene expression of human EPHA8

    Depositor

    Markku Varjosalo

    Article

    Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

    Insert

    EPHA8 (EPHA8 Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSCIP-hTRAC

    Plasmid

    #154094

    Purpose

    Self-Cutting and Integrating CRISPR backbone targeting human TCR-alpha common chain

    Depositor

    Scott McComb

    Article

    Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

    Insert

    sgRNA and BAIT targeting human TRAC locus
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTS2381-Tier1-PhCMV-Igk_TM

    Plasmid

    #169487

    Purpose

    Tier-1 vector encoding PhCMV-driven transmembrane domain from Junctophilin-1 (TM) for membrane localization of fusion proteins attached to a secretion signal Igk (PhCMV-IgkSS-TM-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven membrane localization
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBTK211

    Plasmid

    #110595

    Purpose

    BTK Type 6 Repressor CDS for golden gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    LacI reverse
    

    Use

    Synthetic Biology

    Available Since

    Feb. 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. myc-hIFITM3-F63Q

    Plasmid

    #104363

    Purpose

    Expresses human IFITM3-F63Q with an N-terminal myc tag in mammalian cells.

    Depositor

    Jacob Yount

    Article

    Chesarino et al EMBO Rep. 2017 Oct;18(10):1740-1751. doi

    Insert

    IFITM3 (IFITM3 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    mutated the following amino acid: F63Q

    Promoter

    CMV

    Available Since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV.U6gLacZ.hSyn.H2B.RFP

    Plasmid

    #170369

    Purpose

    Negative control plasmid used for Crispr/cas9 based disruption. Expressing nuclear RFP.

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

    Insert

    H2B RFP
    

    Use

    AAV

    Promoter

    human Synapsin

    Available Since

    Jan. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBTK103

    Plasmid

    #110582

    Purpose

    BTK Type 2 promoter for golden gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    Lac promoter with lac operator and RBS
    

    Use

    Synthetic Biology

    Available Since

    Feb. 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pMpGWB106-OEP7(1–50)

    Plasmid

    #100602

    Purpose

    Binary vector for expression of Citrine-fused AtOEP7(1-50) (OEP7(1–50)-Citrine) to visualize chloroplast outer envelope membrane in plants

    Depositor

    Yutaka Kodama

    Article

    Tanaka et al J Plant Res. 2017 Jun 20. doi: 10.1007/s

    Insert

    OEP7(1–50)-Citrine (OEP7 Mustard Weed)
    

    Expression

    Plant

    Available Since

    Feb. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTH734-CEN-minHIS3

    Plasmid

    #38227

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Insert

    HIS3 (HIS3 Synthetic, Budding Yeast)
    

    Tags

    HA

    Expression

    Yeast

    Mutation

    Every codon of the coding sequence has been repla…

    Promoter

    TDH3

    Available Since

    Dec. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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