We narrowed to 8,294 results for: construct

1. pGBW-m4046203

   Plasmid

   #145488

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 3C-like proteinase

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 3C-like proteinase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pCGN R-Ras wt

   Plasmid

   #14727

   Depositor

   Adrienne Cox

   Article

   Fiordalisi et al Methods Enzymol. 2001 . 332():3-36.

   Insert

   R-ras (RRAS Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 29, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pGBW-m4046233

   Plasmid

   #149032

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp3

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp3 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;6xHIS

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pTEF1

   Available since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. EF.hICN1

   Plasmid

   #113715

   Purpose

   Constitutive expression of hICN1, e.g. for hedgehog-induction. Based on addgene #17623, with CMV.GFP removed.

   Depositor

   Manfred Ogris

   Article

   Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

   Insert

   None
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGBW-m4046664

   Plasmid

   #149224

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 E (envelope)

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 E (envelope) (E Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pTEF1

   Available since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pLenti-EF1a-Backbone(NN)

   Plasmid

   #27961

   Depositor

   Feng Zhang

   Article

   Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

   Insert

   TAL-Backbone(NN)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGBW-m4133227

   Plasmid

   #152132

   Purpose

   Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;3xFlag

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   CMV

   Available since

   May 20, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pSG84-pLas-TetRssrA

   Plasmid

   #102455

   Purpose

   Expresses TetR protein with ssrA degradation tag. The expression is controlled by a pLas promoter.

   Depositor

   Richard Murray

   Article

   Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

   Insert

   TetR (tetR )
   

   Use

   Tags

   ssrA

   Expression

   Bacterial

   Mutation

   Promoter

   pLas

   Available since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. p5E-ESyn1

   Plasmid

   #80804

   Purpose

   hSyn1 promoter fused to CMVmin

   Depositor

   Philip Washbourne

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   p5E-ESyn1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGBW-m4137386

    Plasmid

    #149540

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Human, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

11. BB1_P_12_asn_mbfA

    Plasmid

    #90289

    Purpose

    BB1 promoter mbfA gene of Aspergillus niger between FS1 and FS2

    Depositor

    Michael Sauer

    Article

    Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

    Insert

    mbfA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLenti-EF1a-Backbone(NI)

    Plasmid

    #27962

    Depositor

    Feng Zhang

    Article

    Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

    Insert

    TAL-Backbone(NI)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGBW-m4252984

    Plasmid

    #153898

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 SARS-CoV-2 E (envelope)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pGBW-m4134900

    Plasmid

    #151911

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 spike prefusion-stabilized

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 spike prefusion-stabilized (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    d1-15 d1209-1273 R682A R685G K986P V987P

    Promoter

    CMV

    Available since

    May 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pLenti-EF1a-Backbone(HD)

    Plasmid

    #27964

    Depositor

    Feng Zhang

    Article

    Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

    Insert

    TAL-Backbone(HD)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSG13-pBAD-TetRssrA

    Plasmid

    #102449

    Purpose

    Expresses TetR protein with ssrA degradation tag. The expression is controlled by a pBAD promoter.

    Depositor

    Richard Murray

    Article

    Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

    Insert

    TetR (tetR )
    

    Use

    Tags

    ssrA

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOGG042

    Plasmid

    #113998

    Purpose

    Vector construction module. Tetracycline resistance module for construction of Golden Gate cloning vectors

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    Tetracycline
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEpic_Lite

    Plasmid

    #84373

    Purpose

    3rd-generation lentiviral destination vector for gateway cloning

    Depositor

    Philip Washbourne

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PL-452 N-EGFP

    Plasmid

    #19173

    Depositor

    Patrik Verstreken

    Article

    Venken et al Nucleic Acids Res. 2008 Aug 1. ():.

    Insert

    EGFP
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pB7WG_ASX

    Plasmid

    #98891

    Purpose

    Plant expression vector for multigene cloning

    Depositor

    Kristina Gruden

    Article

    Lukan et al PLoS One. 2018 Jan 4;13(1):e0190526. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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