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pNN_v2

Plasmid

#32182

Depositor

Feng Zhang

Publication

Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

Insert

TALE Monomer NN

Expression

Bacterial

Available since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pHR_EF1a-->myc-LaG17-tTA synNotch (LA1)

Plasmid

#162230

Purpose

Lentiviral vector for the expression of GFP nanobody LaG17 tTA SynNotch: pHR_EF1a-->myc-LaG17-tTA synNotch (LA1) in mammalian cells

Depositor

Wendell Lim

Publication

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

LaG17 GFP nanobody SynNotch tTA

Use

Lentiviral

Expression

Mammalian

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-N2cG

Plasmid

#100811

Purpose

express rabies virus CVS N2c strain glycoprotein gene (N2cG)

Depositor

Ian Wickersham

Insert

rabies virus CVS N2c strain glycoprotein gene

Expression

Mammalian

Promoter

CAG

Available since

Feb. 10, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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AAEL010097-Cas9

Plasmid

#100707

Purpose

The promoter of AAEL010097 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

Depositor

Omar Akbari

Publication

Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

Inserts

AAEL010097-hspcas9-GFP
Opie2-dsRed

Use

CRISPR

Tags

GFP and dsRED

Expression

Insect

Promoter

AAEL010097 and Opie2

Available since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTG-mTDG

Plasmid

#52266

Purpose

Bacterial expression of mouse TDG with C-terminal GST-tag

Depositor

Primo Schaer

Publication

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Insert

TDG (Tdg Mouse)

Tags

GST

Expression

Bacterial

Promoter

T7

Available since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pMAL-c2x-MT1

Plasmid

#32101

Depositor

David DeRosier, Chris Mercogliano, Cristina Risco Ortiz

Publication

Mercogliano et al J Struct Biol. 2007 Oct;160(1):70-82. Ep

Insert

Metallothionein (Mt1 Mouse)

Tags

MBP

Expression

Bacterial

Promoter

P-lac

Available since

Jan. 13, 2012

Availability

Academic Institutions and Nonprofits only
CVB3 IRES

Plasmid

#189968

Purpose

Part 2 plasmid to introduce the CVB3 IRES into circRNAs

Depositor

Howard Chang

Publication

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

CVB3 IRES

Use

Cloning

Promoter

N/A

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-EFS-PCP-3XNeon-nls

Plasmid

#75386

Purpose

PCP-3XNeon-nls

Depositor

Thoru Pederson

Publication

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

PCP

Use

Lentiviral

Tags

3XmNeonGreen

Expression

Mammalian

Promoter

EFS

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Frb-N-TEV

Plasmid

#100095

Purpose

SNIPer plasmid, N terminal TEV fused to Frb

Depositor

Jim Wells

Publication

Morgan et al Proc Natl Acad Sci U S A. 2015 Jul 7;112

Insert

N-TEV

Use

Retroviral

Tags

Frb and Myc

Expression

Mammalian

Mutation

N terminal domain

Promoter

CMV

Available since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pXP422

Plasmid

#26845

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Publication

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

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1307 pcDNA3 GFP PTEN A4

Plasmid

#10760

Depositor

William Sellers

Publication

Vazquez et al J Biol Chem. 2001 Dec 28. 276(52):48627-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PTEN (PTEN Human)

Tags

GFP

Expression

Mammalian

Mutation

A4: S380A, T382A, T383A, S385A

Available since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
lenti.Cas9.BFP.Blast

Plasmid

#196714

Purpose

WT SpCas9 with BSD-TagBFP for selection and sorting

Depositor

Bernhard Schmierer

Insert

Cas9-T2A-BSD-TagBFP

Use

CRISPR and Lentiviral

Tags

Nucleoplasmin NLS and SV40 NLS

Promoter

EF1a

Available since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDonor14-N7HELIX-KanR

Plasmid

#190663

Purpose

Kanamycin resistance donor for N7HELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence

Depositor

Benjamin Kleinstiver

Publication

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

KanR, R6K origin of replication

Expression

Bacterial

Promoter

NA

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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lenti-25xAARE-minP-EGFP

Plasmid

#159666

Purpose

EGFP reporter plasmid containing 25 tandem repeats of the amino acid response element (AARE)

Depositor

Seiichi Oyadomari

Publication

Kitakaze et al Elife. 2019 Dec 17;8. pii: 43302. doi: 1

Insert

25xAARE-minP-EGFP

Use

Lentiviral

Expression

Mammalian

Available since

March 5, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR221-NR1H3

Plasmid

#79514

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human NR1H3 CDS

Depositor

Peter Jon Nelson

Publication

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

LXR Alpha (NR1H3 Human)

Use

Gateway Cloning

Available since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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883 pET15b PTEN

Plasmid

#10936

Depositor

William Sellers

Insert

PTEN (PTEN Human)

Tags

His

Expression

Bacterial

Available since

Jan. 3, 2006

Availability

Academic Institutions and Nonprofits only
pXP418

Plasmid

#26843

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Publication

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available since

Feb. 3, 2011

Availability

Academic Institutions and Nonprofits only

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pLL7.0: mTiam1(64-437)-tgRFPt-SSPB R73Q

Plasmid

#60418

Purpose

Mammalian Expression of Tiam DH/PH-tgRFPt-Micro

Depositor

Brian Kuhlman

Publication

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

mTiam1-tgRFPt-SSPB R73Q

Use

Cre/Lox and Lentiviral

Tags

tgRFPt

Expression

Mammalian

Mutation

mTiam 64-437 // SSPB R73Q, Y11K and A15E

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pPS22 (pET23a-Cas10/Csm/csm2H6N)

Plasmid

#70038

Purpose

Bacterial expression of the S. epidermidis Cas10·Csm complex

Depositor

Luciano Marraffini

Publication

Hatoum-Aslan et al J Biol Chem. 2013 Sep 27;288(39):27888-9

Insert

Cas10/Csm complex

Tags

6xHIS on N terminus of Csm2

Expression

Bacterial

Promoter

T7

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCAG-CFP-SaCas9-HF (without sgRNA)

Plasmid

#134470

Purpose

Human expression vector for SaCas9-HF variant.

Depositor

Zongli Zheng

Publication

Tan et al Proc Natl Acad Sci U S A. 2019 Sep 30. p

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Staphylococcus aureus Cas9 nuclease (SauCas9) with high genome-wide specificity

Use

CRISPR

Tags

SV40NLS-3xFLAG

Expression

Mammalian

Mutation

R245A, N413A, N419A, R654A

Promoter

CAG

Available since

Nov. 26, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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