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pDEST17-UbE2B

Plasmid

#15781

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2B (UBE2B Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
pBS Wnt-3aHA

Plasmid

#17374

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-3aHA (Wnt3a Mouse)

Tags

HA

Expression

Bacterial

Available Since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
pLentiGuide

Plasmid

#117986

Purpose

pLentiGuide is a gRNA insert lentiviral vector, with a stuffer sequence for BsmBI cloning of annealed oligos corresponding to sgRNA sequence. It has Puromycin resistance and Ampicilin resistance.

Depositor

Paul Khavari

Article

Rubin et al Cell. 2018 Dec 11. pii: S0092-8674(18)31

Insert

sgRNA backbone (F+E)

Use

Lentiviral

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-GFAP104-ChR2-mCherry

Plasmid

#58892

Purpose

AAV-mediated expression of ChR2-mCherry under the GFAP promoter

Depositor

Edward Boyden

Article

Perea et al Nat Commun. 2014;5:3262. doi: 10.1038/nc

Has Service

AAV5

Insert

ChR2-mCherry

Use

AAV

Tags

mCherry

Expression

Mammalian

Promoter

GFAP104

Available Since

Aug. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA4-PKCZeta WT His tagged

Plasmid

#24609

Depositor

Jeff Wrana

Article

Wang et al Science. 2003 Dec 5. 302(5651):1775-9.

Insert

PKCZeta (PRKCZ Human)

Tags

His

Expression

Mammalian

Available Since

May 5, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pLVX.TRE3G.AdProt

Plasmid

#131687

Purpose

A lentiviral vector for tTA-regulated expression of the adenovirus protease using the tetracycline transactivator transcription factor.

Depositor

Matthew D. Shoulders

Article

Berman et al J Am Chem Soc. 2018 Dec 26;140(51):18093

Insert

Adenovirus Protease

Use

Lentiviral

Promoter

TRE3GV

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pMyNT-kan

Plasmid

#84693

Purpose

Protein expression in mycobacteria

Depositor

Matthias Wilmanns

Article

Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

Type

Empty backbone

Tags

His6

Expression

Bacterial

Promoter

Acetamidase

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pFH2.105_PB-EPN24-MCP-T2A-eGFP-PGK-Blast

Plasmid

#205550

Purpose

Express EPN24-MCP-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN24-MCP

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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FRP718_PACT1(-1-520)-LexA-ER-haB42-TCYC1

Plasmid

#58431

Purpose

Expresses LexA-ER-B42 in yeast cells

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

LexA-ER-B42

Expression

Yeast

Promoter

Act1 promoter (-520;1)

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pRK5-GRK6

Plasmid

#32693

Depositor

Robert Lefkowitz

Article

Pitcher et al J Biol Chem. 1996 Oct 4. 271(40):24907-1

Insert

GRK6 (GRK6 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2012

Availability

Academic Institutions and Nonprofits only

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FRB-mCherry-FTH1

Plasmid

#100749

Purpose

Part of FerriTag expression system. Encodes human Ferritin with an FRB domain and mCherry tag

Depositor

Stephen Royle

Article

Clarke et al Nat Commun. 2018 Jul 4;9(1):2604. doi: 1

Insert

ferritin heavy chain 1 (FTH1 Human)

Tags

FRB and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pEF-Bos-FLAG MDA5 MI

Plasmid

#52875

Purpose

Expresses MDA5 with a mutation to motif I in mammalian cells

Depositor

Curt Horvath

Article

Bamming et al J Biol Chem. 2009 Apr 10;284(15):9700-12

Insert

IFIH1 (IFIH1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

K335A, E658K

Available Since

May 9, 2014

Availability

Academic Institutions and Nonprofits only

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Flag‐hTim1

Plasmid

#49207

Purpose

expresses Flag-tagged human Tim1

Depositor

Lawrence Kane

Article

de Souza et al Proc Natl Acad Sci U S A. 2005 Nov 22;10

Insert

Tim1 (HAVCR1 Human)

Tags

FLAG and signal sequence

Expression

Mammalian

Mutation

Tim1 lacks its own signal sequence

Promoter

EF1a

Available Since

Dec. 11, 2013

Availability

Academic Institutions and Nonprofits only
pSF4 CMV 5TOP intron renilla TRICK CTE polyA

Plasmid

#64544

Purpose

5′ TOP TRICK reporter mRNA

Depositor

Jeffrey Chao

Article

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

12x PP7 stem-loops

Tags

24xMS2 stem loops, 5' TOP (5′ terminal oligo…

Expression

Mammalian

Promoter

Tetracycline-inducible promoter and 5′ UTR of hum…

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-SomArchon

Plasmid

#126941

Purpose

Expression of SomArchon under Syn promoter

Depositor

Edward Boyden

Article

Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

Insert

SomArchon

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

Synapsin

Available Since

June 24, 2019

Availability

Academic Institutions and Nonprofits only

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pCaST-elav-VP16AD

Plasmid

#15308

Depositor

Benjamin White

Article

Luan et al Neuron. 2006 Nov 9. 52(3):425-36.

Insert

VP16AD-ZIP+

Expression

Insect

Available Since

July 6, 2007

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-Backbone(NI)

Plasmid

#27962

Depositor

Feng Zhang

Article

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TAL-Backbone(NI)

Use

Lentiviral

Expression

Mammalian

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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MCK-luciferase

Plasmid

#16062

Depositor

Robert Benezra

Article

Markus et al J Biol Chem. 2002 Feb 22. 277(8):6469-77

Insert

MCK enhancer (CKM Human)

Tags

Luciferase

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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pActPL-Gal4AD

Plasmid

#15303

Depositor

Benjamin White

Article

Luan et al Neuron. 2006 Nov 9. 52(3):425-36.

Insert

Gal4AD-ZIP+ (GAL4 )

Expression

Insect

Available Since

July 6, 2007

Availability

Academic Institutions and Nonprofits only

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pDONR223-MARK2

Plasmid

#23404

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MARK2 (MARK2 Human)

Use

Gateway Cloning

Mutation

aa1-399

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only