We narrowed to 5,994 results for: crispr cas9 expression plasmids

1. pCRISPR-cBEST-v2-kasOP*

   Plasmid

   #209447

   Purpose

   improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the kasOP* promoter

   Depositor

   Tilmann Weber

   Article

   Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021

   Insert

   Codon optimized APOBEC1-nCas9-UGI fusion protein
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   PtipA; sgRNA expression from kasOP* promoter

   Available since

   March 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRISPR-cBEST-v2-PermE

   Plasmid

   #209446

   Purpose

   improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site

   Depositor

   Tilmann Weber

   Article

   Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021

   Insert

   Codon optimized APOBEC1-nCas9-UGI fusion protein
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   PtipA; sgRNA expression from ermE* promoter

   Available since

   March 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. PAX6 CKO 2/7 Donor plasmid

   Plasmid

   #68467

   Purpose

   donor plasmid for targeting PAX6 locus

   Depositor

   Su-Chun Zhang

   Article

   Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

   Insert

   FRT flanked exon4 of PAX6 gene (PAX6 Human)
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse PGK promoter

   Available since

   Nov. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDCC5

   Plasmid

   #59984

   Purpose

   Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9-D10A nickase and U6>sgRNA cassette

   Depositor

   Peter Duchek

   Article

   Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

   Insert

   Cas9-D10A
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Insect

   Mutation

   D10A

   Promoter

   hsp70Bb

   Available since

   Oct. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV:ITR-U6-sgRNA (Backbone) PCB-FlPO-WPRE-syntetisk pA-UTR

   Plasmid

   #68347

   Purpose

   AAV plasmid expressing the FlpO recombinase. with empty gRNA

   Depositor

   Jannik Elverløv-Jakobsen

   Insert

   FlPO
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFREE

   Plasmid

   #92050

   Purpose

   Allows inducible expression of gRNAs and Cas9 for plasmid curing.

   Depositor

   Morten Norholm

   Article

   Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

   Inserts

   Cas9
   gRNA array
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pRhamBAD and ptet

   Available since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pITI2

   Plasmid

   #166095

   Purpose

   Gal inducible expression of D10A-Cas9 nickase

   Depositor

   Rebecca Burgess

   Insert

   Cas9-D10A
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   spCas9-D10A

   Promoter

   Gal-inducible

   Available since

   June 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPH3

   Plasmid

   #166098

   Purpose

   Gal inducible expression of D10A-Cas9 nickase

   Depositor

   Rebecca Burgess

   Insert

   Cas9-D10A
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   spCas9-D10A

   Promoter

   Gal-inducible

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFREE_Zeo

   Plasmid

   #92053

   Purpose

   Allows inducible expression of gRNAs and Cas9 for plasmid curing.

   Depositor

   Morten Norholm

   Article

   Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

   Inserts

   Cas9
   gRNA array
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   pRhamBAD and ptet

   Available since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFREE_Amp

    Plasmid

    #92051

    Purpose

    Allows inducible expression of gRNAs and Cas9 for plasmid curing.

    Depositor

    Morten Norholm

    Article

    Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

    Inserts

    Cas9
    gRNA array
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    pRhamBAD and ptet

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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