We narrowed to 11,551 results for: NSI

1. pAB061i10

   Plasmid

   #79227

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   Ppro1 rpoZ-HA4 (Y87R); PlacIq lacI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ppro1; PlacIq

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAB061i11

   Plasmid

   #79228

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   Ppro1 rpoZ-HA4 (Y87K); PlacIq lacI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ppro1; PlacIq

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAB061i8

   Plasmid

   #79231

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   Ppro1 rpoZ-HA4 (Y87W); PlacIq lacI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ppro1; PlacIq

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAB061i9

   Plasmid

   #79232

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   Ppro1 rpoZ-HA4 (Y87F); PlacIq lacI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ppro1; PlacIq

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAB082c

   Plasmid

   #79235

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   Ppro1 rpoZ-Cry1Ac-d2A
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ppro1

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAB082d

   Plasmid

   #79236

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   Ppro1 rpoZ-Cry1Ac-d2B
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ppro1

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAB082e

   Plasmid

   #79237

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   Ppro1 rpoZ-Cry1Ac-d2C
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ppro1

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAB082g

   Plasmid

   #79239

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   Ppro1 rpoZ-Cry1Ac-d23B
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ppro1

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAB094b

   Plasmid

   #79242

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PBAD rpoZ-HA4 (Y35A); PC araC
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PBAD; PC

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAB094c

    Plasmid

    #79243

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PBAD rpoZ-HA4 (R38A); PC araC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD; PC

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAB094d

    Plasmid

    #79244

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PBAD rpoZ-HA4 (E52A); PC araC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD; PC

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBABE-hygro-mTurquoise2-Nuf2

    Plasmid

    #80762

    Purpose

    Express Nuf2 N-terminally labeled with mTurquoise2

    Depositor

    Daniel Needleman

    Article

    Yoo et al Elife. 2018 Jul 25;7. pii: 36392. doi: 1

    Insert

    Nuf2 (NUF2 Human)
    

    Use

    Retroviral

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. FH95pUP95S73DGW (F3)

    Plasmid

    #74040

    Purpose

    lentiviral expression of Psd95 shRNA and Psd95-S73D-EGFP fusion

    Depositor

    Robert Malenka, Weifeng Xu, Bernardo Sabatini

    Article

    Steiner et al Neuron. 2008 Dec 10;60(5):788-802. doi:

    Insert

    Psd95
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    S73D

    Promoter

    H1

    Available Since

    Availability

    Academic Institutions and Nonprofits only

14. pRK5_tAKARδ

    Plasmid

    #119916

    Purpose

    Mammalian expression of the tAKARδ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

    Depositor

    Haining Zhong

    Article

    Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

    Insert

    tAKARδ
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRK5_tAKARε

    Plasmid

    #119917

    Purpose

    Mammalian expression of the tAKARε sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

    Depositor

    Haining Zhong

    Article

    Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

    Insert

    tAKARε
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. NaVSp1 Ae1 CC pIRES

    Plasmid

    #128079

    Purpose

    Mammalian cell expression vector for (NaVSp1 1-236 NaVAe1 265-288) chimera bacterial sodium channel.

    Depositor

    Dan Minor

    Article

    Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

    Insert

    NaV Sp1 and NaVAe1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. NaVSp1 Bh1 CTD pIRES

    Plasmid

    #128080

    Purpose

    Mammalian cell expression vector for (NaVSp1 1-220 NaVBh1 236-274) chimera bacterial sodium channel.

    Depositor

    Dan Minor

    Article

    Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

    Insert

    NavSp1_Bh1CTD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. NaVSp1 Ms CTD pIRES

    Plasmid

    #128081

    Purpose

    Mammalian cell expression vector for (NaVSp1 1-220 NaVMs 222-274) chimera bacterial sodium channel.

    Depositor

    Dan Minor

    Article

    Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

    Insert

    NavSp1_MsCTD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. NaVSp1 Pz CTD pIRES

    Plasmid

    #128083

    Purpose

    Mammalian cell expression vector for (NaVSp1 1-220 NaVPz 224-262) chimera bacterial sodium channel.

    Depositor

    Dan Minor

    Article

    Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

    Insert

    NavSp1_PzCTD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. NaVAe1 Sp1 CTD pIRES

    Plasmid

    #128085

    Purpose

    Mammalian cell expression vector for (NaVAe1 1-243 NaVSp1 220-255) chimera bacterial sodium channel.

    Depositor

    Dan Minor

    Article

    Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

    Insert

    NaVAe1 and Sp1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLIB-HISX6-TEV-RSP4

    Plasmid

    #176693

    Purpose

    Expression of Chlamydomonas reinhardtii RSP4 recombinant protein in insect cells

    Depositor

    Ron Vale

    Article

    Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

    Insert

    RSP4
    

    Use

    Tags

    HISX6

    Expression

    Insect

    Mutation

    None

    Promoter

    polyhedrin

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLIB-RSP5

    Plasmid

    #176694

    Purpose

    Expression of Chlamydomonas reinhardtii RSP5 recombinant protein in insect cells

    Depositor

    Ron Vale

    Article

    Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

    Insert

    RSP5 (RSP5 Chlamydomonas reinhardtii)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    None

    Promoter

    polyhedrin

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBR9-genomic_RSP6-HAX3

    Plasmid

    #176703

    Purpose

    Rescue of RSP6 Chlamydomonas mutant with tagged RSP6

    Depositor

    Ron Vale

    Article

    Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

    Insert

    RSP6
    

    Use

    Tags

    HAX3

    Expression

    Plant

    Mutation

    None

    Promoter

    rbcs2

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pNEU-KlacRS

    Plasmid

    #232155

    Purpose

    Site-specific incorporation of L-lactyl-lysine into target proteins in mammalian cells.

    Depositor

    Nanxi Wang

    Article

    Shao et al Nat Commun. 2025 Jan 8;16(1):227. doi: 1

    Insert

    KlacRS1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA-mCherry-TAG-EGFP

    Plasmid

    #232156

    Purpose

    A fluorescence reporter to show incorporation of target unnatural amino acids.

    Depositor

    Nanxi Wang

    Article

    Shao et al Nat Commun. 2025 Jan 8;16(1):227. doi: 1

    Insert

    mCherry-linker(TAG)-EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Profiline

    Plasmid

    #89925

    Purpose

    Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin

    Depositor

    Guillaume Romet-Lemonne, Antoine Jégou

    Article

    Jegou et al PLoS Biol. 2011 Sep;9(9):e1001161. doi:

    Insert

    profiline-1 (PFN1 Human)
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. GFP-PKCA D463N

    Plasmid

    #195203

    Purpose

    Mammalian expression vector for GFP tagged PKCA. Kinase-inactive mutant

    Depositor

    Peter Parker, David Bryant

    Article

    Cameron et al Nat Struct Mol Biol. 2009 Jun;16(6):624-

    Insert

    PRKCA (PRKCA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D463N

    Promoter

    Available Since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pmEGFP-C1-Nir3-LNS2

    Plasmid

    #220087

    Purpose

    C-terminal PA binding domain of Nir3

    Depositor

    Gerry Hammond

    Article

    Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

    Insert

    PITPNM2 (PITPNM2 Human)
    

    Use

    Tags

    mEGFP-GGGGSHM

    Expression

    Mammalian

    Mutation

    amino acids 925-1209

    Promoter

    CMV

    Available Since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBAD-HisD-KRaION1

    Plasmid

    #177799

    Purpose

    Bacterial expression of green fluorescent potassium indicator KRaION1

    Depositor

    Edward Boyden

    Article

    Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

    Insert

    KRaION1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araB

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. AAV_pMBP-EGFP-caax

    Plasmid

    #190155

    Purpose

    Expresses membrane-targeted EGFP in oligodendrocytes; AAV vector

    Depositor

    Brad Zuchero

    Article

    Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

    Insert

    EGFP-caax
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MBP

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3-ExRai-AMPKAR

    Plasmid

    #192446

    Purpose

    Genetically encoded excitation-ratiometric fluorescent biosensor for monitoring AMPK activity in living cells.

    Depositor

    Jin Zhang

    Article

    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

    Insert

    ExRai-AMPKAR
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Tn7 helper plasmid

    Plasmid

    #141161

    Purpose

    Temperature-sensitive plasmid carries tnsABCD genes that encode transposase biochemical machinery under an arabinose-inducible pBAD promoter

    Depositor

    Shimon Bershtein

    Article

    Jasinska et al Nat Ecol Evol. 2020 Feb 24. pii: 10.1038

    Insert

    Tn7 transposon machinery
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Arabinose-inducible pBAD

    Available Since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pEGB 35S:Renilla:Tnos (GB0109)

    Plasmid

    #68215

    Purpose

    Transcriptional unit for Renilla Luciferase Protein plant expression driven by the 35S promoter

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Renilla
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    35S

    Available Since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAAV-TRE-DIO-ChR2-TdTomato

    Plasmid

    #166600

    Purpose

    Encodes Cre-dependent ChR2-TdTomato under control of the TRE

    Depositor

    Martin Myers

    Article

    Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

    Insert

    ChR2-TdTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    H134R

    Promoter

    tetracycline response element

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. EK0393 SFFV-mTagBFP2-SGc(EGFP.s2)cSG-mCherry (FLP-IN)

    Plasmid

    #191159

    Purpose

    Expression of a sensor for EGFP CDS in mammalian cells; mTagBFP2 marker, mCherry output

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mTagBFP:EGFP.s2:mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. EK0313 SFFV-mCherry-SG(s70587)SG-Cre (FLP-IN)

    Plasmid

    #191149

    Purpose

    Expression of Sensor 1 (for EK0208) in mammalian cells; mCherry marker, Cre output derived from a gift from prof. Liqun Luo

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:s70587:Cre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. PB/pHSP-tTA-TRE-tTA-pEF1α-PuroR

    Plasmid

    #206213

    Purpose

    PiggyBac-based transposon vector plasmid which encodes an expression unit of tetracycline-responsive transactivator under control of HSP promoter with transcriptional amplification

    Depositor

    Masamichi Kamihira

    Article

    Kitano et al Cytotechnology. 2021 Jun;73(3):353-362.

    Insert

    tetracycline-responsive transactivator
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3-ExRai-AMPKAR(T/A)

    Plasmid

    #192447

    Purpose

    Non-phosphorylatable negative-control mutant of ExRai-AMPKAR.

    Depositor

    Jin Zhang

    Article

    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

    Insert

    ExRai-AMPKAR(T/A)
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

    Expression

    Mammalian

    Mutation

    Thr 6 in AMPK substrate mutated to Ala.

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAS1NB m Rosella I

    Plasmid

    #71247

    Purpose

    a biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Mitochodrial localization.

    Depositor

    Mark Prescott

    Article

    Rosado et al Autophagy. 2008 Feb;4(2):205-13. Epub 20

    Insert

    Rosella
    

    Use

    Tags

    yeast mitochondrial targeting sequence of citrate…

    Expression

    Yeast

    Mutation

    Promoter

    PGK1

    Available Since

    Nov. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3-Lyso-ExRai-AMPKAR

    Plasmid

    #192449

    Purpose

    ExRai-AMPKAR targeted to the lysosomal membrane.

    Depositor

    Jin Zhang

    Article

    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

    Insert

    Lyso-ExRai-AMPKAR
    

    Use

    Tags

    Full-length LAMP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge