Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 16,165 results for: LEA

Clear keywords
Showing: 2521 - 2540 of 16165 results

  1. U25m

    Plasmid
    #121027
    Purpose
    MoClo golden gate assembly BC part for RiboJ-UTR1 (very strong RBS, prefixed by self-cleaving RiboJ for upstream context independence). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    RiboJ + UTR1
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121027-plasmid-map-sequence-id-238073

    Enlarge

  3. pLG017_Avs1a(I134K)

    Plasmid
    #248945
    Purpose
    AVAST from E. piriflorinigrans with Avs1a mutated (I134K)
    Depositor
    Jennifer Doudna
    Insert
    Avs1a(I134K)
    Expression
    Bacterial
    Mutation
    I134K
    Available Since
    March 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    248945-plasmid-map-sequence-id-504694

    Enlarge

  4. pCas9_antiBFP

    Plasmid
    #220922
    Purpose
    Expresses Cas9 with a BFP targeting crRNA for use in targeting the BFP plasmid in a cell based assay
    Depositor
    Ryan Jackson
    Insert
    pCas9
    Expression
    Bacterial
    Available Since
    March 13, 2026
    Availability
    Academic Institutions and Nonprofits only
    220922-plasmid-map-sequence-id-436286

    Enlarge

  5. pCas9_antiGFP

    Plasmid
    #220923
    Purpose
    Expresses Cas9 with a GFP targeting crRNA for use in targeting the GFP plasmid in a cell based assay
    Depositor
    Ryan Jackson
    Insert
    pCas9
    Expression
    Bacterial
    Available Since
    March 13, 2026
    Availability
    Academic Institutions and Nonprofits only
    220923-plasmid-map-sequence-id-436289

    Enlarge

  6. pCas9_antiOFP

    Plasmid
    #220924
    Purpose
    Expresses Cas9 with a OFP targeting crRNA for use in targeting the OFP plasmid in a cell based assay
    Depositor
    Ryan Jackson
    Insert
    pCas9
    Expression
    Bacterial
    Available Since
    March 13, 2026
    Availability
    Academic Institutions and Nonprofits only
    220924-plasmid-map-sequence-id-436287

    Enlarge

  7. pLG017_Avs1a(I234K)

    Plasmid
    #248946
    Purpose
    AVAST from E. piriflorinigrans with Avs1a mutated (I234K)
    Depositor
    Jennifer Doudna
    Insert
    Avs1a(I234K)
    Expression
    Bacterial
    Mutation
    I234K
    Available Since
    Feb. 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    248946-plasmid-map-sequence-id-500780

    Enlarge

  8. pLG017_Avs1a_chimera

    Plasmid
    #248950
    Purpose
    AVAST from E. piriflorinigrans with Avs1a replaced with Avs1a from Citrobacter Avs1a
    Depositor
    Jennifer Doudna
    Insert
    Avs1a_chimera
    Expression
    Bacterial
    Mutation
    I134K,I234K
    Available Since
    Feb. 19, 2026
    Availability
    Academic Institutions and Nonprofits only
    248950-plasmid-map-sequence-id-500567

    Enlarge

  9. pChillyBoys_gen1

    Plasmid
    #228495
    Purpose
    pACYCDuet-1 encoding Oleispira antarctica GroEL and GorES low temp chaperonins for enhanced low-temperature induction (E. coli based protein expression)
    Depositor
    Richard Baker
    Inserts
    groEL
    groES
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    228495-plasmid-map-sequence-id-454882

    Enlarge

  10. pCer-C3-Plk1

    Plasmid
    #68132
    Purpose
    Expression of Cerulean-tagged Plk1 in mammalian cells
    Depositor
    Catherine Lindon
    Insert
    Polo-like kinase 1 (PLK1 Human)
    Tags
    Cerulean
    Expression
    Mammalian
    Mutation
    wild-type
    Promoter
    CMV
    Available Since
    Oct. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    68132-plasmid-map-sequence-id-123472

    Enlarge

  11. pACYC-GST

    Plasmid
    #62329
    Purpose
    E. coli pACYC based expression vector with N-terminal TEV cleavable GST-tag
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Tags
    Glutathione S-transferase (GST) tag and TEV prote…
    Expression
    Bacterial
    Promoter
    T7-lac0
    Available Since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62329-plasmid-map-sequence-id-296399

    Enlarge

  12. pTet_PflHamMAB(D207A)

    Plasmid
    #248934
    Purpose
    Hachiman from P. fluorescens under pTet control with HamB mutated (D207A)
    Depositor
    Jennifer Doudna
    Insert
    PflHamMAB(D207A)
    Expression
    Bacterial
    Mutation
    D207A
    Available Since
    Feb. 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    248934-plasmid-map-sequence-id-500771

    Enlarge

  13. pLG017_FLAG-Avs1a

    Plasmid
    #248948
    Purpose
    AVAST from E. piriflorinigrans with an N-terminal 3xFLAG tag fused to Avs1a
    Depositor
    Jennifer Doudna
    Insert
    FLAG-Avs1a
    Tags
    3xFLAG
    Expression
    Bacterial
    Available Since
    Feb. 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    248948-plasmid-map-sequence-id-500769

    Enlarge

  14. pTet_PflHamM(I118K)AB

    Plasmid
    #248935
    Purpose
    Hachiman from P. fluorescens under pTet control with HamM mutated (I118K)
    Depositor
    Jennifer Doudna
    Insert
    PflHamM(I118K)AB
    Expression
    Bacterial
    Mutation
    I118K
    Available Since
    Feb. 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    248935-plasmid-map-sequence-id-500767

    Enlarge

  15. pJEX_lambdaterminase

    Plasmid
    #248952
    Purpose
    phage lambda large terminase subunit under control of a crystal violet inducible promoter
    Depositor
    Jennifer Doudna
    Insert
    lambdaterminase
    Expression
    Bacterial
    Available Since
    Feb. 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    248952-plasmid-map-sequence-id-500761

    Enlarge

  16. pTet_PflHamMA(S157A)B

    Plasmid
    #248933
    Purpose
    Hachiman from P. fluorescens under pTet control with HamA mutated (S157A)
    Depositor
    Jennifer Doudna
    Insert
    PflHamMA(S157A)B
    Expression
    Bacterial
    Mutation
    S157A
    Available Since
    Feb. 19, 2026
    Availability
    Academic Institutions and Nonprofits only
    248933-plasmid-map-sequence-id-500565

    Enlarge

  17. pN_UBQ10/mApple

    Plasmid
    #140405
    Purpose
    mApple expression regulated by the A. thaliana UBQ10 promoter, co-expressed nourseothricin acetyl transferase selectable marker (nat)
    Depositor
    Mathias Pribil
    Insert
    mApple
    Use
    Synthetic Biology
    Expression
    Plant
    Promoter
    AtUBQ10
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    140405-plasmid-map-sequence-id-292694

    Enlarge

  18. pHL1726

    Plasmid
    #53032
    Purpose
    PLtetO-1:hfqlead::mCherry, PLlacO-1:RBS(st7):csrA
    Depositor
    Han Lim
    Inserts
    mCherry
    csrA
    Use
    Synthetic Biology
    Tags
    RBS(st7) and hfqlead
    Expression
    Bacterial
    Promoter
    pLlacO-1 and pLtetO-1
    Available Since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  19. pHL1790

    Plasmid
    #53038
    Purpose
    PLtetO-1:glgClead::mCherry, PLlacO-1:csrB
    Depositor
    Han Lim
    Inserts
    mCherry
    csrB
    Use
    Synthetic Biology
    Tags
    glgC leader
    Expression
    Bacterial
    Promoter
    pLlacO-1 and pLtetO-1
    Available Since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  20. pExp-DsbC

    Plasmid
    #112564
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and DsbC (leaderless thiol:disulfide interchange protein DsbC from E. coli) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis-DsbC and Strep tag
    Expression
    Bacterial
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112564-plasmid-map-sequence-id-217214

    Enlarge

Showing: 2521 - 2540 of 16165 results