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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,214 results for: LEA

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Showing: 2521 - 2540 of 12214 results

  1. pGBW-m4046955

    Plasmid
    #145616
    Purpose
    Bacterial Expression plasmid for RNA-dependent RNA polymerase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    RNA-dependent RNA polymerase (Nsp12) (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;FLAG_2
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145616-plasmid-map-sequence-id-307396

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  4. pcDNA3-N-HA-NEK7 K64M

    Plasmid
    #75143
    Purpose
    Expresses N-terminal HA-tagged NEK7 (with a K64M substitution; kinase dead) in mammalian cells
    Depositor
    Bruce Beutler
    Publication
    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7 Mouse)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Lys 64 mutated to Met; kinase inactive and F236L …
    Promoter
    CMV
    Available since
    May 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    75143-plasmid-map-sequence-id-147264

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  5. pcDNA3-blueCKAR

    Plasmid
    #118472
    Purpose
    cpBFP-based single-color biosensor for monitoring Protein Kinase C activity.
    Depositor
    Jin Zhang
    Publication
    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    blueCKAR
    Tags
    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    118472-plasmid-map-sequence-id-230290

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  8. DOT1L

    Plasmid
    #36196
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    DOT1L (GeneScript Synthesized) (DOT1L Human)
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    contains amino acid residues 1-420
    Available since
    Aug. 8, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by

  17. pFHMSP-LIC-N

    Plasmid
    #26095
    Purpose
    Donor vector to generate recombinant baculovirus by site-specific transposition into a baculovirus shuttle vector For use with Bac-to-Bac Expression System insect cells for secreted protein expression
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Baculovirus expression
    Tags
    His and TEV cleavage site
    Expression
    Insect
    Promoter
    polyhedrin
    Available since
    Sept. 15, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 2521 - 2540 of 12214 results