We narrowed to 8,294 results for: construct

1. pCVD433

   Plasmid

   #63756

   Purpose

   Derivative of pACYC184 M1uI linkers inserted into EcoRV site inactivating Tc resistance gene. Now Tcs

   Depositor

   James Kaper

   Article

   Donnenberg et al Infect Immun. 1990 Jun;58(6):1565-71.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. C-SWAT GFP CL1

   Plasmid

   #182512

   Purpose

   C-SWAT compatible vector for C-terminal tagging with GFP followed by the CL1 degron for constitutive gene expression knockdown.

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   CL1 Degron
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGBW-m4046373

   Plasmid

   #145555

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 ORF3a protein

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 ORF3a protein (ORF3a Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
   

   Use

   Tags

   Cleavable thrombin;StrepII

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pGBW-m4133326

   Plasmid

   #152113

   Purpose

   Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;HA

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   CMV

   Available since

   May 20, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pYCTK019

   Plasmid

   #176723

   Purpose

   The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Ste2 gene of Candida albicans (receiver part).

   Depositor

   Victor Sourjik

   Article

   Krink et al bioRxiv 2021

   Insert

   ste2Ca
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Codon-optimized for expression in S. cerevisiae

   Promoter

   Available since

   Jan. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGBW-m4134904

   Plasmid

   #151930

   Purpose

   Mammalian expression plasmid for SARS-CoV-2 spike prefusion-stabilized

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 spike prefusion-stabilized (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   d1-15 d1209-1273 R682A R685G K986P V987P

   Promoter

   CMV

   Available since

   May 20, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. PL-452 C-mDsRed

   Plasmid

   #19179

   Depositor

   Patrik Verstreken

   Article

   Venken et al Nucleic Acids Res. 2008 Aug 1. ():.

   Insert

   mDsRed
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pGBW-m4046299

   Plasmid

   #153878

   Purpose

   Yeast expression plasmid for SARS-CoV-2 S (surface glycoprotein)

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;6xHIS

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pGAL1

   Available since

   June 12, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. PL-452 N-Flag-4C

   Plasmid

   #19175

   Depositor

   Patrik Verstreken

   Article

   Venken et al Nucleic Acids Res. 2008 Aug 1. ():.

   Insert

   FLAG tetracysteine tag
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pGBW-m4134207

    Plasmid

    #152431

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;3xFlag

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available since

    July 30, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. PL-452 C-YPet

    Plasmid

    #19177

    Depositor

    Patrik Verstreken

    Article

    Venken et al Nucleic Acids Res. 2008 Aug 1. ():.

    Insert

    YPet
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pGBW-m4133141

    Plasmid

    #152404

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;3xFlag

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available since

    June 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pGBW-m4258326

    Plasmid

    #154793

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 surface glycoprotein (Spike protein) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;V5

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available since

    July 30, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. PL-452 N-YPet

    Plasmid

    #19172

    Depositor

    Patrik Verstreken

    Article

    Venken et al Nucleic Acids Res. 2008 Aug 1. ():.

    Insert

    YPet
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pGBW-m4252903

    Plasmid

    #153778

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;V5

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pGBW-m4133806

    Plasmid

    #153713

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;S-tag

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pGBW-m4046457

    Plasmid

    #145424

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pGBW-m4046390

    Plasmid

    #148996

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp3

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp3 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available since

    May 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pGBW-m4046332

    Plasmid

    #145413

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 3C-like proteinase

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. PL-452 N-mDsRed

    Plasmid

    #19174

    Depositor

    Patrik Verstreken

    Article

    Venken et al Nucleic Acids Res. 2008 Aug 1. ():.

    Insert

    mDsRed
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2010

    Availability

    Academic Institutions and Nonprofits only