We narrowed to 3,421 results for: mpo

1. p-Tet-O-Sox5-Puro

   Plasmid

   #221667

   Purpose

   Expresses Sox5 in mammalian cells

   Depositor

   Vanessa Hall

   Article

   Bergmann et al Front Cell Dev Biol. 2022 Aug 15;10:9765

   Insert

   Sox5 (Sox5 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-Hygro-EF1A-BRCT2-Linker-eGFP

   Plasmid

   #176085

   Purpose

   EGFP fused to the C-terminus of a BRCT2 domain & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   BRCT2 (XRCC1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-Hygro-EF1A-BRCT1-Linker-eGFP

   Plasmid

   #176074

   Purpose

   EGFP fused to the C-terminus of a BRCT1 domain & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   BRCT1 (XRCC1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. NST1_pOpOn2.1

   Plasmid

   #228065

   Purpose

   Expresses NST1 in different cell types in Arabidopsis thaliana upon induction with dexamethasone.

   Depositor

   Charles Anderson

   Article

   Tamadaddi et al Ann Bot. 2024 Aug 30:mcae153. doi: 10.10

   Insert

   NAC SECONDARY WALL THICKENING PROMOTING FACTOR1, NST1 (NST1 Mustard Weed)
   

   Use

   Dexamethasone inducible vector

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S for LhGR and pOp6-minimal CaMV35S for NST1

   Available since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. p2709-pHAGE2-Ef1aL-YAP5SA-UBC-tagBFP-loxP

   Plasmid

   #216478

   Purpose

   lentiviral dual expression of YAP5SA and tagBFP with loxP site for Cre excision

   Depositor

   Darrell Kotton

   Article

   Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

   Inserts

   YAP5SA
   tagBFP
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mutation

   S61A, S109A, S127A, S164A, S381A

   Promoter

   EF1aL and UBC

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE-mt-mKeima-P2A-FRB-Fis1

   Plasmid

   #135295

   Purpose

   stably express both mKeima and FRB-Fis1 for CID induced mitophagy in mammalian cells

   Depositor

   Richard Youle

   Article

   Vargas et al Mol Cell. 2019 Apr 18;74(2):347-362.e6.

   Insert

   mt-mKeima-P2A-FRB-Fis1 (FIS1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   mt-mKeima is fused with FRB-Fis1 via P2A seqeunce

   Promoter

   Available since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pK27Sumo_His-SUMO-nsp12 (SARS-CoV-2)

   Plasmid

   #169188

   Purpose

   To express His-SUMO-nsp12 (SARS-CoV-2) in E. coli

   Depositor

   John Diffley

   Article

   Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

   Insert

   14His-SUMO-nsp12 (ORF1ab Synthetic, SARS-CoV-2)
   

   Use

   Tags

   14His-SUMO (Ulp1-cleavable)

   Expression

   Bacterial

   Mutation

   Codon optimised for E. coli

   Promoter

   T5

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-OptoFGFR1

   Plasmid

   #59776

   Purpose

   Expresses myristoylation signal peptide-FGFR1 cytoplasmic region-PHR domain of cryptochrome2-mCitrine fused protein

   Depositor

   Won Do Heo

   Article

   Kim et al Chem Biol. 2014 Jul 17;21(7):903-12. doi

   Insert

   OptoFGFR1 (FGFR1 Human, Mustard Weed)
   

   Use

   Tags

   mCitrine

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. CMV-FLAG-TAOK2

   Plasmid

   #197862

   Purpose

   Expression of FLAG-tagged TAOK2 / PSK1 alpha in mammalian cells

   Depositor

   Jonathan Morris

   Article

   Koo et al Mol Cancer Ther. 2017 Nov;16(11):2410-24

   Insert

   TAOK2 (TAOK2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Wild Type

   Promoter

   SV40

   Available since

   March 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-OptoTGFBRs

    Plasmid

    #118942

    Purpose

    Mammalian expression plasmid of OptoTGFBRs (optogenetically-activated TGFb receptors)

    Depositor

    Won Do Heo

    Article

    Li et al ACS Synth Biol. 2018 Feb 16;7(2):443-451

    Insert

    OptoTGFBRs (Tgfb1, Tgfbr2 Mouse, Mustard Weed)
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pK27Sumo_His-SUMO-nsp8 (SARS-CoV-2)

    Plasmid

    #169187

    Purpose

    To express His-SUMO-nsp8 (SARS-CoV-2) in E. coli

    Depositor

    John Diffley

    Article

    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

    Insert

    14His-SUMO-nsp8 (ORF1ab Synthetic, SARS-CoV-2)
    

    Use

    Tags

    14His-SUMO (Ulp1-cleavable)

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T5

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMRXIP-GFP-CERT(PHD)(W33A)

    Plasmid

    #221761

    Purpose

    Stable expression of GFP-CERT(PHD)(W33A) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    CERT (CERT1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    amino acids 1–116, mutated 33W to 33A

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNK2657

    Plasmid

    #219741

    Purpose

    MoClo-compatible Level 0 promoterless vector encoding hispidin-synthase from Mycena citricolor codon-optimised for expression in Nicotiana benthamiana, Pichia pastoris, Homo sapiens

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    hispidin- synthase from Mycena citricolor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCOLADuet-1_nsp10 (SARS-CoV-2)

    Plasmid

    #169158

    Purpose

    To express SARS-CoV-2 nsp10 in E. coli

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    nsp10 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Methionine

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T7

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV_CMV_CCR6-Nanoluc-V5

    Plasmid

    #199672

    Purpose

    expresses CCR6-NanoLuc-V5 in mammalian cells, AAV vector

    Depositor

    Alice Ting

    Article

    Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

    Insert

    CCR6 (CCR6 Human)
    

    Use

    AAV

    Tags

    NanoLuc and V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNK115

    Plasmid

    #219745

    Purpose

    MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 codon-optimised for expression in Nicotiana benthamiana

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    mutant of fungal luciferase
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mutation

    I3S, N4T, F11L, I63T, T99P, T192S, A199P

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNK116

    Plasmid

    #219742

    Purpose

    MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Nicotiana benthamiana

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    mutant of fungal hispidin-3-hydroxylase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    D37E, V181I, S323M, M385K

    Promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. BcLOV4 fusion tools screening plasmid #2

    Plasmid

    #174512

    Purpose

    Cloning plasmid for creating BcLOV4 optogenetic tool fusions. Expresses [BamHI]-mCherry-[XhoI]-GGGSx2-BcLOV4-[EcoRI]-GGGS-3xFLAG-STOP in a pcDNA3.1 backbone.

    Depositor

    Brian Chow

    Article

    Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

    Insert

    plasmid B [BamHI]-mCherry-[XhoI]-GGGSx2-BcLOV4-[EcoRI]-GGGS-3xFLAG-STOP
    

    Use

    Tags

    3xFLAG and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. HT107_pAAV_hSyn-DiO-SomQuasAr6a_EGFP-P2A-somCheRiff_HA

    Plasmid

    #178826

    Purpose

    Cre-on bicistronic expression of QuasAr6b-based Optopatch under an neuronal promoter

    Depositor

    Adam Cohen

    Article

    Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

    Insert

    SomQuasAr6a_EGFP-P2A-somCheRiff_HA
    

    Use

    AAV, Cre/Lox, and Mouse Targeting

    Tags

    EGFP; HA

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-CMV-EGFP-PolB-PAMmut-Hygro

    Plasmid

    #176086

    Purpose

    EGFP fused to the N-terminus of a POLB containing a mutation in the PAM site used by POLBKOg1 & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Mutation in the PAM site used by PolBKOg1

    Promoter

    CMV

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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