We narrowed to 10,315 results for: plasmids 101

1. AAVS1-pur-CAG-Bi-DREADD

   Plasmid

   #159457

   Purpose

   AAVS1 targeting donor plasmid with Bi-DREADD expression cassette (hM3Dq-mCherry-P2A-HA-KORD) and puromycin selection gene

   Depositor

   Yuejun Chen

   Article

   Xiong et al Cell Stem Cell. 2020 Sep 16. pii: S1934-

   Insert

   Bi-DREADD
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   fusion protein of hM3Dq-mCherry and HA-KORD seper…

   Promoter

   CAG

   Available Since

   Dec. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CMV-FLEX-SaCas9-U6-sgTh(2)

   Plasmid

   #209198

   Purpose

   Mutagenesis of Th

   Depositor

   Larry Zweifel

   Article

   Yu et al Neuron. 2021 Apr 21;109(8):1365-1380.e5.

   Insert

   Th (Th Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pαH-S-GSAS

   Plasmid

   #164565

   Purpose

   SARS-CoV-2 Spike protein (S-GSAS variant)

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

   Insert

   Spike (S-GSAS variant)
   

   Use

   Tags

   2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

   Expression

   Mammalian

   Mutation

   Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

   Promoter

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pαH-S-RRAR/D614G

   Plasmid

   #164570

   Purpose

   SARS-CoV-2 Spike protein with D614G mutation and Furin site restored (S-RRAR-D614G variant)

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

   Insert

   Spike (S-RRAR-D614G variant)
   

   Use

   Tags

   2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

   Expression

   Mammalian

   Mutation

   D614G

   Promoter

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOGS539_GLuc-ns

   Plasmid

   #226719

   Purpose

   Plasmid enabling yeast-mediated expression of Gaussia luciferase (GLuc)

   Depositor

   Gautam Dantas

   Article

   Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

   Insert

   Gaussia Luciferase
   

   Use

   Tags

   HA tag

   Expression

   Yeast

   Mutation

   Promoter

   pTEF1

   Available Since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-TRE3_ mTFP1- Vamp2

   Plasmid

   #201214

   Purpose

   AAV expression of TRE(TRE3G)-driven, Vamp2-conjugated mTFP1 expression for fluorescent labling of axon terminals

   Depositor

   Tetsuo Yamamori

   Article

   Watakabe et al Neuron. 2023 May 9:S0896-6273(23)00338-0

   Insert

   mTFP1 (from addgene #54613)
   

   Use

   AAV

   Tags

   mouse Vamp2

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   TRE3G

   Available Since

   July 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pαH-S-HRV3C/D614G

   Plasmid

   #164568

   Purpose

   SARS-CoV-2 Spike protein with furin site mutated to HRV3C site and D614G mutation (S-HRV3C-D614G variant)

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

   Insert

   Spike (S-HRV3C-D614G variant)
   

   Use

   Tags

   2X Strep-Tag II and 8X His tag

   Expression

   Mammalian

   Mutation

   D614G

   Promoter

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. R619-M67-303: CMV51p> SARS-CoV-2 S-RBD(319-541)-His6

   Plasmid

   #166018

   Purpose

   mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD)

   Depositor

   Dominic Esposito

   Article

   Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

   Insert

   SARS-CoV-2 RBD (S )
   

   Use

   Tags

   His6

   Expression

   Mammalian

   Mutation

   Promoter

   CMV51

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Tnnt2 -Atp2a2-HA

   Plasmid

   #227804

   Purpose

   cardiomyocytes-specific plasmid with Tnnt2 promotor, expressing the coding sequence of mouse Atp2a2 and a HA tag

   Depositor

   Yuxuan Guo

   Article

   Chen et al Fundam Res. 2025 Jun 30. doi: https://do

   Insert

   Atp2a2-HA (Atp2a2 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tnnt2

   Available Since

   Aug. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. ENTR_BN001T-N_START

    Plasmid

    #218058

    Purpose

    RESOLUTE SLC binder cloning plasmid

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

    Insert

    BN001T-N_START
    

    Use

    Cloning plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. ENTR_BN006A-P_START

    Plasmid

    #218059

    Purpose

    RESOLUTE SLC binder cloning plasmid

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

    Insert

    BN006A-P_START
    

    Use

    Cloning plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. ENTR_BN001M-T_START

    Plasmid

    #218060

    Purpose

    RESOLUTE SLC binder cloning plasmid

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

    Insert

    BN001M-T_START
    

    Use

    Cloning plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. AF10_OM-LZ

    Plasmid

    #111753

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Article

    Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

    Insert

    MLLT10 (MLLT10 Human)
    

    Use

    Tags

    6x His - TEV

    Expression

    Bacterial

    Mutation

    Contains the OM-LZ region of AF10 (residues 720-7…

    Promoter

    T7

    Available Since

    June 14, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pCRISPEY-Z3-Hyg

    Plasmid

    #232101

    Purpose

    Yeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHW1221

    Plasmid

    #229873

    Purpose

    gene trap RMCE plasmid with QF-act-4 3' UTR::SEC for RMCE cloned in the destination vector pHW1133 to swap driver via RMCE

    Depositor

    Han Wang

    Article

    Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.

    Insert

    QF-act-4 3' UTR::SEC
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. JD180

    Plasmid

    #229486

    Purpose

    Adenoviral helper plasmid containing inducible E2A and L4-22k/33k genes

    Depositor

    Nerea Zabaleta

    Article

    Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

    Insert

    E2A, L4-22k/33k, mCitrine
    

    Use

    AAV and Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tre (E2A, L4-22k/33k), ETR (mCitrine)

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. JD136

    Plasmid

    #229497

    Purpose

    Adenoviral helper plasmid containing E2A and L4-22k/33k and VA RNA I genes

    Depositor

    Nerea Zabaleta

    Article

    Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

    Insert

    E2A, L4-22k/33k, VA RNA I
    

    Use

    AAV and Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    wt promoter

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-Ef1a-Coff/Fon-GCaMP6F

    Plasmid

    #137124

    Purpose

    Intersectional viral expression of GCaMP6F in cells expressing Flp AND NOT Cre

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Coff/Fon-GCaMP6F
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    Removed RSET tag

    Promoter

    EF1a

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-Ef1a-Coff/Fon-mCherry

    Plasmid

    #137134

    Purpose

    Intersectional viral expression of mCherry in cells expressing Flp AND NOT Cre

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Coff/Fon-mCherry
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    n/a

    Promoter

    EF1a

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-Ef1a-Con/Fon-oScarlet

    Plasmid

    #137136

    Purpose

    Intersectional viral expression of oScarlet in cells expressing both Cre AND Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Fon-oScarlet
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    E95D

    Promoter

    EF1a

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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