We narrowed to 17,207 results for: puro

1. Ef1a-somBiPOLES-mCerulean-IRES-Puro

   Plasmid

   #192585

   Purpose

   lentiviral vector for bidirectional optogenetic manipulation of Cre-positive neurons

   Depositor

   Simon Wiegert

   Insert

   somBiPOLES
   

   Use

   Lentiviral

   Tags

   soma-targeting motif from Kv2.1 channel

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1-alpha

   Available since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYJ21-lenti-EF1a-ASAP2f-puro

   Plasmid

   #131065

   Purpose

   Forced expression of ASAP2f

   Depositor

   Xiaojun Lian

   Article

   Jiang et al Stem Cell Res Ther. 2021 May 29;12(1):30

   Insert

   ASAP2f
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO-Tet-puro-hARAF-shRNA-1

   Plasmid

   #185370

   Purpose

   For tetracycline-inducible mammalian expression of shRNA: GCCGTGACCAGATTATCTTTA that targets human ARAF

   Depositor

   Ayaz Najafov

   Insert

   ARAF (ARAF Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBABE-2xFLAG-2xSTREP-CCND2-Puro

   Plasmid

   #172628

   Purpose

   Retroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D2 and a puromycin resistance marker in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND2 (CCND2 Human)
   

   Use

   Retroviral

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. LV UAS-DsRed-Express2-PGK-Puro

   Plasmid

   #190801

   Purpose

   LV construct encoding UAS-DsRed-Express2 reporter gene.

   Depositor

   John Ngo

   Article

   Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

   Insert

   UAS-T2A-DsRed-Express2
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL3-U6-sgRNA-PGK-puromycin-AflII

   Plasmid

   #106404

   Purpose

   Empty guide RNA cloning vector with an AflII site added from Addgene 41824

   Depositor

   Jason Gertz

   Article

   Carleton et al Cell Syst. 2017 Oct 25;5(4):333-344.e5.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBabe-TetCMV-puro-mCerulean-PA-Rac1

   Plasmid

   #22031

   Depositor

   Klaus Hahn

   Article

   Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

   Insert

   mCerulean-PA-Rac1 (RAC1 Avena sativa (oat), Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Rac1 starts at I4 and contains mutations Q61L, E9…

   Promoter

   Available since

   Sept. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

8. pLKO.1-Tet-Puro-shMMERVK10c_1 (inducible)

   Plasmid

   #189955

   Purpose

   shRNA mediated knockdown

   Depositor

   Denes Hnisz

   Article

   Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

   Insert

   ERV_MMERVK10c
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLEX-MAPKAP1.1-FHH-IRES-Puro

   Plasmid

   #120705

   Purpose

   Expresses MAPKAP1.1-Flag-HA-6xHIS fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   MAPKAP1 Isoform 1
   

   Use

   Lentiviral

   Tags

   Flag-HA-6xHIS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPuro3.1(+)_Strep-Lrrk2(delGTPase)-Myc

    Plasmid

    #161587

    Purpose

    expression of Myc-tagged mouse Lrrk2 without GTPase domain

    Depositor

    Maik Hintze

    Insert

    Lrrk2 (Lrrk2 Mouse)
    

    Use

    Tags

    Myc tag and StrepII

    Expression

    Mammalian

    Mutation

    deletion: AA1023-1879

    Promoter

    CMV

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPuro3.1(+)_Strep-Lrrk2(delKinase)-Myc

    Plasmid

    #161586

    Purpose

    expression of Myc-tagged mouse Lrrk2 without kinase domain

    Depositor

    Maik Hintze

    Insert

    Lrrk2 (Lrrk2 Mouse)
    

    Use

    Tags

    Myc tag and StrepII

    Expression

    Mammalian

    Mutation

    deletion: AA1871-2540

    Promoter

    CMV

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. p7270 MSCV-N-HA-IRES-PURO Empty

    Plasmid

    #163302

    Purpose

    Empty vector control

    Depositor

    Elizabeth White, Peter Howley

    Article

    White et al J Virol. 2014 Aug;88(15):8201-12. doi: 1

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Direct-seq_lentiGuide-Puro-Tail-8A8G

    Plasmid

    #157981

    Purpose

    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

    Depositor

    Lijia Ma

    Article

    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

    Insert

    S. pyogenes sgRNA cassette (Tail-8A8G)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIVTR(T7A)-CRE-T2A-PuroR

    Plasmid

    #172298

    Purpose

    In vitro transcription of CRE-T2A-PuroR synthetic construct for the excision of floxed cassettes

    Depositor

    Patrik Verstreken

    Insert

    CRE-T2A-PuroR
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter A-initiating

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. 1120 pBabe puroL HA FKHR H215R

    Plasmid

    #9026

    Depositor

    William Sellers

    Article

    Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

    Insert

    FKHR H215R (FOXO1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    H215R

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pKI-cjFOXL2-coemiRFP670-PGK-Puro

    Plasmid

    #186168

    Purpose

    Knock-in gene targeting in marmoset cells at FOXL2 locus

    Depositor

    Sho Yoshimatsu

    Article

    Yoshimatsu et al Biology (Basel). 2022 Jun 29;11(7):987.

    Insert

    emiRFP670
    

    Use

    Marmoset targeting

    Tags

    Expression

    Mutation

    human codon-optimized

    Promoter

    Available since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBabe puro H-Ras 12V (37G)

    Plasmid

    #12589

    Depositor

    Channing Der

    Article

    McFall et al Mol Cell Biol. 2001 Aug . 21(16):5488-99

    Insert

    HRas 12V 37G (HRAS Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    12V, 37G

    Promoter

    Available since

    Sept. 21, 2006

    Availability

    Academic Institutions and Nonprofits only

18. LentiU6-mNeurog2 gRNA_1-MS2-Puro

    Plasmid

    #192680

    Purpose

    Lentiviral expression of sgRNA targeting mIL1RN promoter to activate mouse Neurog2 transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Mouse Neurog2 activating gRNA #1 (Neurog2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBRPy CAGGS-Mbd3-IRES-PURO

    Plasmid

    #52376

    Purpose

    Mammalian expression vector for constitutive expression of Mbd3

    Depositor

    Jacob Hanna

    Article

    Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

    Insert

    Mbd3 (Mbd3 Mouse)
    

    Use

    Tags

    2xFlag (DYKDDK)

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKLV2-U6gRNA5(hYAP1-Y2B)-PGKpuro2ABFP-W

    Plasmid

    #163178

    Purpose

    Lentiviral gRNA plasmid targeting human YAP1 gene, co-expression of BFP tag

    Depositor

    Sok Ching Cheong

    Article

    Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

    Insert

    YAP1 (YAP1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    BFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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