173,273 results
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Plasmid#219564Purpose3` circularization cassette.To induce Cre-mediated circularization or inversion of a genomic region with concomitant GFP reconstitution and mScarlet expression from the chromosomeDepositorInsert3` CC: Hygro – GFP-N – loxP – mScarlet
UseUnspecifiedPromoterEF1-aAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGBScrispri Sham
Plasmid#223201PurposeGroup B Streptococcus inducible dCas12 CRISPRi with Modular crRNADepositorInsertN/A
UseCRISPRAvailable SinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV4-BlaM-Vpr
Plasmid#21950DepositorInsertBlaM-Vpr (vpr Fusion of BlaM and HIV-1 Vpr)
ExpressionMammalianAvailable SinceOct. 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
KG#865
Plasmid#110912PurposeCoding sequence for attaching the Auxin Inducible Degron to the C-terminus of a protein followed by a 5X Myc tag with spacersDepositorInsertAuxin Inducible Degron-5X Myc-Spacers for C-terminal attachment
ExpressionBacterialAvailable SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET22b_PstS_1
Plasmid#78198PurposeExpression of single-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensorDepositorInsertpstS (A197C)
ExpressionBacterialMutationA197C (please see depositors comment below)PromoterT7Available SinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.CMVs.Pl.Cre.rBG
Plasmid#105537PurposeAAV expression of Cre from CMV promoterDepositorHas ServiceAAV rh10, AAV1, AAV2, AAV5, AAV8, and AAV9InsertCre
UseAAV and Cre/LoxExpressionMammalianPromoterCMVAvailable SinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKSE401
Plasmid#62202PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Kan resistanceDepositorInsertsgRNA scaffold
zCas9
UsePlant binary vectorTags3x FLAG and NLSExpressionPlantMutationmaize codon optimizedPromoter35S and AtU6-26pAvailable SinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEcCas
Plasmid#73227PurposeConstitutive expression of cas9 and inducible expression of lambda RED and sgRNADepositorInsertsCas9
sgRNA
UseCRISPR; Red recombinaseExpressionBacterialPromoternative promoterAvailable SinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDual_dsCas9_Puro
Plasmid#214680PurposeLentiviral expression vector for an inducible Cas9-P2A-Puromycin resistance casette with two empty sgRNA cloning sitesDepositorTypeEmpty backboneUseCRISPR and LentiviralAvailable SinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSET-MagLOV
Plasmid#219957PurposeBacterial expression of MagLOV, a magnetic-field-sensitive fluorescent protein engineered from AsLOV2DepositorInsertMagLOV
Tags6xHisExpressionBacterialMutationAsLOV2 C450P L496V Q513K G528K D540MPromoterT7Available SinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only