We narrowed to 29,679 results for: REP

1. pJRH051

   Plasmid

   #171625

   Purpose

   2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA (no spacer, BsmBI cutsites) and an EF1-a promoter expressing mNeonGreen

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-BsmBI-Destination
   mNeon
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-a and U6

   Available Since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGL4.10-VEGFprom(-1000 to -1)

   Plasmid

   #66128

   Purpose

   Luciferase-based reporter for VEGF promoter region (-1000 to -1 bp)

   Depositor

   David Mu

   Article

   Wood et al Sci Rep. 2016 Feb 25;6:19857. doi: 10.10

   Insert

   Vascular Endothelial Growth Factor -A promoter region (VEGFA Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   VEGF -1000 to -1

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-EF1a-double floxed-Gat1-HA-WPRE-HGHpA

   Plasmid

   #184635

   Purpose

   Expresses the GABA membrane transporter Gat1 fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashion

   Depositor

   Nicolas Tritsch

   Article

   Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

   Insert

   Gat1 (Slc6a1 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLVX-IRES-Hygro_RGECO1.2

   Plasmid

   #164592

   Purpose

   Stable integration and expression of RGECO1.2 in mammalian cells

   Depositor

   Joseph Hyser

   Article

   Chang-Graham et al Sci Rep. 2019 Jul 25;9(1):10822. doi: 10

   Insert

   R-GECO1.2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   R-GECO1 M164R/I166V/V174L/F222L/N267S/S270T/I330M…

   Promoter

   CMV

   Available Since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. -5.5kb-UCP1-GFP

   Plasmid

   #104585

   Purpose

   Reporter assay to monitor the transcription activity of the UCP1 promoter by GFP expression

   Depositor

   Irfan Lodhi

   Article

   Lodhi et al Cell Rep. 2017 Sep 19;20(12):2766-2774.

   Insert

   UCP1 promoter (Ucp1 Mouse)
   

   Use

   Tags

   TurboGFP

   Expression

   Mammalian

   Mutation

   Promoter

   UCP1

   Available Since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. SuperNova/pRSETB

   Plasmid

   #53234

   Purpose

   monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation

   Depositor

   Takeharu Nagai

   Article

   Takemoto et al Sci Rep. 2013;3:2629. doi: 10.1038/srep0

   Insert

   monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation
   

   Use

   Tags

   6xHis, T7 tag, and Xpress tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. 3xFLAG-CUL4A-pCMV7.1

   Plasmid

   #155022

   Purpose

   N-terminal 3xFLAG-tagged CUL4A in a pCMV7.1 vector

   Depositor

   Andre Catic

   Article

   Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

   Insert

   Cullin 4A (CUL4A Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Bacterial

   Mutation

   Promoter

   pCMV7.1

   Available Since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. eGFP-KIF5B-Rigor

   Plasmid

   #172204

   Purpose

   Expresses KIF5B G234A rigor mutant tagged with eGFP in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Guardia et al Cell Rep. 2016 Nov 15;17(8):1950-1961. d

   Insert

   KIF5B (Kif5b Mouse)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV CaMKIIa iChloC 2A tDimer

   Plasmid

   #66709

   Purpose

   Chloride-conducting Channelrhodopsin with neuron-specific promoter, plus red fluorescent protein

   Depositor

   Thomas Oertner

   Article

   Wietek et al Sci Rep. 2015 Oct 7;5:14807. doi: 10.103

   Inserts

   Channelrhodopsin-2
   red fluorescent protein
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   E83Q,E90R,E101S,D156N,T159C

   Promoter

   CaMKIIa

   Available Since

   Oct. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Green Pegassos

    Plasmid

    #163114

    Purpose

    Expresses biosensors for pyruvate in mammalian cells

    Depositor

    Takashi Tsuboi

    Article

    Harada et al Sci Rep. 2020 Nov 11;10(1):19562. doi: 1

    Insert

    Green Pegassos
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-EF1a-DIO-EGFP-WPRE

    Plasmid

    #187103

    Purpose

    Double floxed EGFP under the control of EF1a promoter

    Depositor

    Yongling Zhu

    Article

    Xu et al Cell Rep. 2022 Jul 5;40(1):111036. doi:

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CLYBL-TO-PAX7

    Plasmid

    #204346

    Purpose

    Knock-in vector to insert a dox-inducible PAX7 transgene into the CYBL locus in human pluripotent stem cells. PAX7 induction results in myogenic differentiation.

    Depositor

    Ivo Lieberam

    Article

    Harley et al Cell Rep. 2023 Dec 26;42(12):113509. doi

    Insert

    human PAX7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available Since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMSCV-NLS-CBRed-d2-hygro

    Plasmid

    #139196

    Purpose

    non-ERK dependent control plasmid for pMSCV-NLS-CBGreen-FIRE-puro

    Depositor

    Matthew Lazzara

    Article

    Day et al Cell Rep. 2020 Mar 10;30(10):3383-3396.e

    Insert

    CBRed
    

    Use

    Luciferase and Retroviral

    Tags

    d2 PEST domain and nuclear localization signal (N…

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDEST40-2XFL-Src-K298M

    Plasmid

    #140315

    Purpose

    Expresses Flag-tagged human Src-K298M in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src mutant (SRC Human)
    

    Use

    Tags

    2X Flag

    Expression

    Mammalian

    Mutation

    K298M

    Promoter

    CMV

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBJB20 - eft-3p::mitofilin::Mac::GFP::unc-54 3'UTR

    Plasmid

    #158967

    Purpose

    Ubiquitous expression of mtON in C. elegans

    Depositor

    Andrew Wojtovich

    Article

    Berry et al EMBO Rep. 2020 Apr 3;21(4):e49113. doi:

    Insert

    eft-3p::mitofilin::Mac::GFP::unc-54 3'UTR
    

    Use

    Tags

    GFP

    Expression

    Worm

    Mutation

    Promoter

    eft-3

    Available Since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLVX-puro_G-CEPIA1er

    Plasmid

    #164590

    Purpose

    Stable integration and expression of G-CEPIA1er in mammalian cells

    Depositor

    Joseph Hyser

    Article

    Chang-Graham et al Sci Rep. 2019 Jul 25;9(1):10822. doi: 10

    Insert

    G-CEPIA1er
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    G-GECO1.1 E299D M304L D328N N345K F360W G369S E37…

    Promoter

    CMV

    Available Since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-CAG-mTagBFP2-P2A-EGFP

    Plasmid

    #184943

    Purpose

    Expresses blue fluorescent protein mTagBFP2 and EGFP in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    mTagBFP2-P2A-EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCDNA3_Sec61B-V5-APEX2

    Plasmid

    #83411

    Purpose

    Expressed C-term tagged APEX2 on Sec61B in mammalian cell

    Depositor

    Hyun-Woo Rhee

    Article

    Lee et al Cell Rep. 2016 May 11. pii: S2211-1247(1

    Insert

    Sec61B-V5-APEX2 (SEC61B Human, Synthetic)
    

    Use

    Tags

    APEX2 and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-F

    Available Since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEGFP_WT-talin1 (1-2541)

    Plasmid

    #166112

    Purpose

    For expression of full-length wild-type mouse talin-1 in mammalian cells. Contains C-terminal EGFP tag.

    Depositor

    Vesa Hytönen

    Article

    Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

    Insert

    WT-Talin-1 (Tln1 Mouse)
    

    Use

    Cmv promoter

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDEST40-2XHA-Src-Y530F

    Plasmid

    #140318

    Purpose

    Expresses HA-tagged human Src-Y530F in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src mutant (SRC Human)
    

    Use

    Tags

    2X HA

    Expression

    Mammalian

    Mutation

    Y530F

    Promoter

    CMV

    Available Since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-CAG-mBlueberry2-P2A-EGFP

    Plasmid

    #184937

    Purpose

    Expresses blue fluorescent protein mBlueberry2 and EGFP in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    mBlueberry2-P2A-EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCDNA3.1 (-) + SLNCR1 + 12x MS2 bs

    Plasmid

    #86828

    Purpose

    Expression of SLNCR1 lncRNA tagged with 12 copies of the MS2 stem-loop sequence

    Depositor

    Carl Novina

    Article

    Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

    Insert

    LINC00673 (LINC00673 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGEX nsp1 CoV2

    Plasmid

    #175512

    Purpose

    For protein expression of WT nsp1 CoV2

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    nsp1 CoV2
    

    Use

    Tags

    GST tag

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Tom70 (1-70)-GFP

    Plasmid

    #127633

    Purpose

    Mitochondrial targeting sequence of Tom70 fused to GFP

    Depositor

    Josef Kittler

    Article

    Covill-Cooke et al EMBO Rep. 2020 Feb 5;21(2):e49865. doi:

    Insert

    Tom70 (TOMM70 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Only first 70 amino acids

    Promoter

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCSIIbsr-Eevee-ROCK-NES

    Plasmid

    #209918

    Purpose

    A FRET biosensor for ROCK activity

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    Eevee-ROCK-NES
    

    Use

    Lentiviral

    Tags

    ECFP and YPet

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET151-GyrA

    Plasmid

    #216229

    Purpose

    S. aureus USA300 GyrA Codon-optimized for E. coli

    Depositor

    Eric Brown

    Article

    French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

    Insert

    DNA gyrase
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pENTR221-FOS

    Plasmid

    #79511

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human FOS CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    FOS (FOS Human)
    

    Use

    Multisite gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    no Promoter

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. SGEN

    Plasmid

    #111171

    Purpose

    miR-E (miR-30 variant)-based RNAi

    Depositor

    Johannes Zuber

    Article

    Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

    Insert

    miR-E (miR-30 variant)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRubiC-T2A-Cas9

    Plasmid

    #75347

    Purpose

    Ubiquitin promoter expresses mCherry and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.

    Depositor

    Bryan Luikart

    Article

    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

    Insert

    Cas9
    

    Use

    CRISPR and Retroviral

    Tags

    mCherry (via t2a)

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pKRG3-mU6-PUb-hSpCas9

    Plasmid

    #162162

    Purpose

    Expresses hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)

    Depositor

    Charles Rice

    Article

    Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

    Inserts

    hSpCas9
    sgRNA
    

    Use

    CRISPR

    Tags

    N/A

    Expression

    Insect

    Mutation

    Promoter

    Ae. aegypti PUb and Ae. aegypti U6

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge