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pHAGE-NLS-dPspCas13b-3xmRuby3-NLS

Plasmid

#132405

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Expression

Mammalian

Mutation

H133A; H1059A

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pSB-CMV-MCS-Puro dr_cPLA2-mKate2

Plasmid

#163844

Purpose

Used as a driver for the readout of cPLA2 activation in live cells with an mKate2 tag.

Depositor

Philipp Niethammer

Article

Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

Insert

cytosolic phospholipase a2 (pla2g4aa Zebrafish)

Tags

mKate2

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCX:Gachapin-4A-P2A-mCherry-NLS

Plasmid

#251133

Purpose

Gachapin-A with a transfection marker mCherry-NLS

Depositor

Takeharu Nagai

Article

Kanadome et al Cell Rep Methods. 2026 Jan 23:101292. do

Insert

Gachapin-4A

Tags

P2A-mCherry-NLS

Expression

Mammalian

Promoter

Chicken β-actin promoter

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pCX:Gachapin-4B-P2A-EBFP2-NLS

Plasmid

#251134

Purpose

Gachapin-B with a transfection marker EBFP2-NLS

Depositor

Takeharu Nagai

Article

Kanadome et al Cell Rep Methods. 2026 Jan 23:101292. do

Insert

Gachapin-4B

Tags

P2A-EBFP2-NLS

Expression

Mammalian

Promoter

Chicken β-actin promoter

Available Since

Feb. 18, 2026

Availability

Academic Institutions and Nonprofits only

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pBluescriptSKII+ U6-sgRNA(F+E) empty

Plasmid

#74707

Purpose

Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and BbsI sites that allow insertion of a spacer sequence

Depositor

Eugene Yeo

Article

Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

Insert

U6 promoter driving sgRNA with Bbsi sites for insertion of spacer sequence

Expression

Mammalian

Available Since

July 15, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_eGFP

Plasmid

#26822

Depositor

Derrick Rossi

Article

Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

Insert

5'UTR-eGFP-3'UTR

Expression

Mammalian

Available Since

Nov. 17, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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Cas9 sgRNA vector

Plasmid

#68463

Purpose

Cas9 sgRNA vector for cloning

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

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Flag-GFP-HRP-TM_pLX304

Plasmid

#202021

Purpose

expresses GFP-HRP anchored to the extracellular side of the plasma membrane, lentiviral vector

Depositor

Alice Ting

Article

Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

Insert

Flag-GFP-HRP-TM

Use

Lentiviral

Tags

Flag and GFP

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmp7

Plasmid

#163591

Purpose

Expresses BMP-7 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmp7 (Bmp7 Mouse)

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pLX208_CMV::mCherry-myc-VAMP2

Plasmid

#199716

Purpose

Expresses VAMP2, fused to mCherry, in mammalian cells. Can be used in generating stable cell lines via lentiviral transduction.

Depositor

Alice Ting

Article

Roh et al J Am Chem Soc. 2023 May 10;145(18):10220

Insert

mCherry-myc-VAMP2

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1aL-mCherry-W

Plasmid

#126703

Purpose

Constitutive, ubiquitous mCherry expression.

Depositor

Darrell Kotton

Article

McCauley et al Cell Stem Cell. 2017 Jun 1;20(6):844-857

Insert

mCherry

Use

Lentiviral

Promoter

Ef1aL

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pT3-Neo-EF1a-GFP

Plasmid

#69134

Purpose

Sleeping Beaquty (SB) vector encoding G418 resistance and GFP from two separate promoters

Depositor

Martin Bonamino

Article

Chicaybam et al Front Bioeng Biotechnol. 2017 Jan 23;4:9

Type

Empty backbone

Use

Sleeping beauty transposon

Expression

Mammalian

Promoter

U3MSCV

Available Since

Jan. 19, 2017

Availability

Academic Institutions and Nonprofits only

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PL552

Plasmid

#68407

Purpose

Donor empty vector with floxed puromycin resistance gene expression cassette

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Type

Empty backbone

Use

Cre/Lox

Expression

Mammalian

Promoter

mouse PGK promoter

Available Since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pHAGE-NLS-dPguCas13b-3xmRuby3-NLS

Plasmid

#132406

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPguCas13b

Use

Lentiviral

Expression

Mammalian

Mutation

H151A; H1121A

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLOVE-miR302/367

Plasmid

#31310

Depositor

Ed Morrisey

Article

Anokye-Danso et al Cell Stem Cell. 2011 Apr 8. 8(4):376-88.

Insert

mmu-miR302/367

Use

Lentiviral

Expression

Mammalian

Mutation

None

Available Since

Oct. 25, 2011

Availability

Academic Institutions and Nonprofits only
pEB-Tg

Plasmid

#28220

Depositor

Linzhao Cheng

Article

Chou et al Cell Res. 2011 Mar;21(3):518-29. doi: 10

Insert

SV40 large T

Expression

Mammalian

Available Since

April 20, 2011

Availability

Academic Institutions and Nonprofits only

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LentiGuidPuro-hTP53_sgRNA-1

Plasmid

#88853

Purpose

CRISPR KO of Trp53

Depositor

Joan Massague

Article

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Trp53

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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AICSDP-19:LAMP1-mEGFP

Plasmid

#101782

Purpose

Homology arms and linker-mEGFP sequence for C-terminus tagging of human LAMP1

Depositor

Allen Institute for Cell Science

Insert

LAMP1 Homology Arms with linker-mEGFP (LAMP1 Human)

Use

CRISPR; Donor template

Tags

linker-mEGFP

Expression

Mammalian

Available Since

Nov. 15, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pLV-EF1a-mCherry-MinD-IRES-Puro

Plasmid

#213112

Purpose

Expression of mCherry-MinD in mammalian cells

Depositor

Scott Coyle

Article

Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

Insert

mCherry-MinD (minD Escherichia coli)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-Akt

Plasmid

#39531

Depositor

Julian Downward

Article

Watton et al Curr Biol. 1999 Apr 22;9(8):433-6.

Insert

Akt (Akt1 Mouse)

Tags

6G linker and EGFP

Expression

Mammalian

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only