We narrowed to 11,617 results for: SHA

1. pEF1a-2xSV40_NLS-CeNLuc

   Plasmid

   #135952

   Purpose

   Expresses the CeNLuc LumiFluor targeted to the nucleus in mammalian cells.

   Depositor

   Antonio Amelio

   Article

   Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

   Insert

   CeNLuc LumiFluor
   

   Use

   Tags

   2xSV40-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEF1a-T2-CeNLuc

   Plasmid

   #135948

   Purpose

   Expresses the CeNLuc LumiFluor targeted to the mitochondira in mammalian cells.

   Depositor

   Antonio Amelio

   Article

   Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

   Insert

   CeNLuc LumiFluor
   

   Use

   Tags

   T2 mitochondrial localization sequence

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRG/III-hs-MBP-A2aTr316-H10

   Plasmid

   #121683

   Purpose

   Bacterial expression of adenosine A2a receptor fusion protein

   Depositor

   Reinhard Grisshammer, Richard Henderson

   Article

   Weiss et al Eur J Biochem. 2002 Jan;269(1):82-92.

   Insert

   adenosine A2a receptor (ADORA2A Human)
   

   Use

   Tags

   His10

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRII-TOPO CMV-cGFP-SV40 Poly(A) Sense

   Plasmid

   #46836

   Purpose

   Express cGFP transcript ending in a poly(A) tail [using the SV40 poly(A) signal]

   Depositor

   Phil Sharp

   Article

   Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

   Insert

   Coral Green Fluorescent Protein (cGFP)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-NIrD-Mefv iso1

   Plasmid

   #92108

   Purpose

   inducible expression in eukaryotic cells

   Depositor

   Feng Shao

   Article

   Gao et al Proc Natl Acad Sci U S A. 2016 Aug 16;11

   Insert

   Mefv isoform1 (Mefv Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMMP-puro-Flag-HA-NR6A1

   Plasmid

   #46833

   Depositor

   Phil Sharp

   Article

   Gurtan et al Genes Dev. 2013 Apr 15;27(8):941-54. doi

   Insert

   NR6A1 (Nr6a1 Mouse)
   

   Use

   Retroviral

   Tags

   Flag-HA, plus 8-amino acid residue linker

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Nov. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

7. eDrTrkA

   Plasmid

   #183996

   Purpose

   Optically controlled eDrTrkA expression in mammalian cells

   Depositor

   Vladislav Verkhusha, Dmitry Gerashchenko

   Article

   Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

   Insert

   eDrTrkA (NTRK1 Human)
   

   Use

   Tags

   Ig kappa secretory signal, mCherry, Deinococcus r…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLaNGo11-1

   Plasmid

   #126053

   Purpose

   A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell

   Depositor

   Ralf-Bernd Klösgen

   Article

   Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

   Inserts

   chloroplastic FNR transit peptide
   ccdB cassette (BsaI)
   GFP11
   

   Use

   Binary

   Tags

   GFP1-10

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCEP4-myc-H2-Ld

   Plasmid

   #135519

   Purpose

   Mammalian expression of myc-tagged H2-Ld

   Depositor

   Erik Procko

   Article

   McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

   Insert

   H2-Ld
   

   Use

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHJ424

    Plasmid

    #20682

    Depositor

    Stephen Shaw

    Article

    Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

    Insert

    Ezrin (EZR Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed Lys 253, 254, 262, 263 to Asn

    Promoter

    Available since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

11. FLAG HDAC5 S259A

    Plasmid

    #32214

    Depositor

    Reuben Shaw

    Article

    Mihaylova et al Cell. 2011 May 13;145(4):607-21.

    Insert

    HDAC5 (HDAC5 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser259Ala

    Promoter

    CMV

    Available since

    Nov. 2, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pBAD-BpBBS

    Plasmid

    #199467

    Purpose

    Arabinose-induced expression of His-tagged biliverdin-binding serpin of Boana punctata treefrog (BpBBS) in E. coli.

    Depositor

    Vladislav Verkhusha

    Article

    Manoilov et al J Mol Biol. 2022 Jan 30;434(2):167359. d

    Insert

    BpBBS
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. EF1a_SPIC_P2A_Hygro_Barcode

    Plasmid

    #120484

    Purpose

    Barcoded lentiviral vector to express SPIC in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    SPIC (SPIC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLAMP1-miRFP718nano

    Plasmid

    #195749

    Purpose

    LAMP1 (Lysosome Membrane) labelled with near-infrared fluorescent protein miRFP718nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

    Insert

    LAMP1-miRFP718nano
    

    Use

    Tags

    miRFP718nano

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pActin-miRFP718nano

    Plasmid

    #195745

    Purpose

    Human beta-actin labelled with near-infrared fluorescent protein miRFP718nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

    Insert

    miRFP718nano-human beta-actin
    

    Use

    Tags

    miRFP718nano

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMC_mNG2(11)_BSDminus_F1

    Plasmid

    #184163

    Purpose

    Donor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 1, as well as a blasticidin resistance gene for selection of genomic integrants.

    Depositor

    Ophir Shalem

    Article

    Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

    Inserts

    mNG2(11)
    bsd
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-T5-ASG_ClpXP

    Plasmid

    #153299

    Purpose

    Expresses acoustic sensor genes or ClpXP-sensitive gas vesicles in E. coli Nissle 1917

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

    Insert

    ASG
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. p3xFlag-CMV14-hISD11

    Plasmid

    #31897

    Depositor

    Gino Cortopassi

    Article

    Shan et al Hum Mol Genet. 2007 Apr 15;16(8):929-41.

    Insert

    ISD11 (LYRM4 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pPN022

    Plasmid

    #91668

    Purpose

    Express sgRNA targeting human SHANK3

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    SHANK3 (SHANK3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNIR-Fb6m

    Plasmid

    #184680

    Purpose

    NIR-Fb to spike-Cov-19 RBD

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    NIR-Fb6m (S Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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