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pmCherry-NLS

Plasmid

#39319

Depositor

Martin Offterdinger

Article

Micutkova et al Int J Cancer. 2012 Apr 1;130(7):1544-57.

Insert

mCherry-3xNLS

Use

Tags

3xSV40LT NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 26, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV-PEmax-P2A-GFP

Plasmid

#180020

Purpose

Mammalian expression of SpCas9 PEmax prime editor with P2A-EGFP marker

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

PEmax-P2A-EGFP

Use

Tags

P2A-EGFP, SV40 bpNLS, and c-Myc NLS

Expression

Mammalian

Mutation

Detailed in manuscript

Promoter

CMV

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgRNA

Plasmid

#124844

Purpose

Vector for Cre-dependent expression of SaCas9

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

SaCas9, gRNA scaffold

Use

AAV, CRISPR, and Mouse Targeting

Tags

NLS and NLS-3xHA

Expression

Mutation

Promoter

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-YFP

Plasmid

#11180

Purpose

Mammalian expression vector for expression of YFP

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Insert

EYFP from Aequorea victoria

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 13, 2006

Availability

Academic Institutions and Nonprofits only

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pLEX_307

Plasmid

#41392

Purpose

Constitutive lentiviral expression, SV40-puro; EF1a-gateway-V5 tag

Depositor

David Root

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral; Gateway destination vector

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pU6-pegRNA-GG-acceptor

Plasmid

#132777

Purpose

Prime editing in mammalian cells

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

Exchangeable cassette

Use

Tags

Expression

Mammalian

Mutation

See manuscript

Promoter

U6

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG RABV-G

Plasmid

#36398

Depositor

Connie Cepko

Article

Beier et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

Insert

rabies virus glycoprotein

Use

Tags

Expression

Mutation

Promoter

CAGGs

Available since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only
pYL156 (TRV RNA2)

Plasmid

#148969

Purpose

Modified Tobacco Rattle Virus RNA 2; used for virus induced gene silencing in plants along with pYL192 (TRV RNA1)

Depositor

Savithramma Dinesh-Kumar

Article

Liu et al Plant J. 2002 May;30(4):415-29. doi: 10.

Insert

Modified TRV RNA2

Use

T-dna vector

Tags

Expression

Mutation

see comments section below

Promoter

Available since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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pSLQ8001 - pCMV-R8.91 (D64V)

Plasmid

#202687

Purpose

R8.91 plasmid with mutated integrase (D64V)

Depositor

Stanley Qi

Article

Chavez et al Nat Biomed Eng. 2023 May 1. doi: 10.1038

Insert

gag pol

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

D64V

Promoter

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pRT029

Plasmid

#127969

Purpose

double-DHFR-degron controlled expression of dCas9-BFP-KRAB from the CLYBL locus

Depositor

Martin Kampmann

Article

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Insert

CLYBL-CAG-DHFR-dCas9-BFP-KRAB-DHFR

Use

TALEN

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 24, 2019

Availability

Academic Institutions and Nonprofits only

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