We narrowed to 584 results for: pcmv gfp

1. pCMV-OptoSTIM1(Cry2(D387A))

   Plasmid

   #70160

   Purpose

   Light-insensitive mutant of OptoSTIM1

   Depositor

   Won Do Heo

   Article

   Kyung et al Nat Biotechnol. 2015 Sep 14. doi: 10.103

   Insert

   OptoSTIM1(Cry2(D387A)) (STIM1 Mustard Weed, Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-ABE-P48R

   Plasmid

   #161816

   Purpose

   Expresses ABE-P48R in mammalian cells

   Depositor

   Sangsu Bae

   Insert

   bpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A in S. pyogenes Cas9, TadA mutations describe…

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV-SpdCas9-VP64

   Plasmid

   #115794

   Purpose

   Expresses SpdCas9-Vp64 fusion protein when packaged into AAV

   Depositor

   Nadav Ahituv

   Article

   Matharu et al Science. 2018 Dec 13. pii: science.aau06

   Insert

   VP64
   

   Use

   AAV

   Tags

   3XFLAG and dCas9-VP64

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-mTagBFP2-PLCδ-PH

   Plasmid

   #214275

   Purpose

   Mammalian expression of blue fluorescent PI(4,5)P2 reporter mTagBFP2-PLCδ-PH

   Depositor

   Shinya Tsukiji

   Article

   Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

   Insert

   mTagBFP2-PLCδ(1-175) (PLCD1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-iRFP713-ORP9-PH

   Plasmid

   #214269

   Purpose

   Mammalian expression of near-IR fluorescent PI4P reporter iRFP713-ORP9-PH

   Depositor

   Shinya Tsukiji

   Article

   Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

   Insert

   iRFP713-ORP9(1-111) (Osbpl9 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-dTomato-ORP9-PH

   Plasmid

   #214268

   Purpose

   Mammalian expression of red fluorescent PI4P reporter dTomato-ORP9-PH

   Depositor

   Shinya Tsukiji

   Article

   Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

   Insert

   dTomato-ORP9(1-111) (Osbpl9 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-mTagBFP2-ORP9-PH

   Plasmid

   #214267

   Purpose

   Mammalian expression of blue fluorescent PI4P reporter mTagBFP2-ORP9-PH

   Depositor

   Shinya Tsukiji

   Article

   Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

   Insert

   mTagBFP2-ORP9(1-111) (Osbpl9 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-GalT-mCherry

   Plasmid

   #214271

   Purpose

   Mammalian expression of GalT-mCherry (Golgi marker)

   Depositor

   Shinya Tsukiji

   Article

   Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

   Insert

   GalT(1-82)-mCherry (B4GALT1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-LAMP1-mCherry

   Plasmid

   #214274

   Purpose

   Mammalian expression of LAMP1-mCherry (lysosome marker)

   Depositor

   Shinya Tsukiji

   Article

   Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

   Insert

   LAMP1-mCherry (Lamp1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV AURKA-mTurq2

    Plasmid

    #157767

    Purpose

    Expression of AuroraA kinase fused to mTurquoise2 under CMV promoter

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-ABE-P48R-UGI

    Plasmid

    #161817

    Purpose

    Expresses ABE-P48R-UGI in mammalian cells

    Depositor

    Sangsu Bae

    Insert

    bpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-10AA linker-UGI-10AA linker-UGI-bpNLS-P2A-EGFP-NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Promoter

    Available since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV-mCherry-MHC-IIA

    Plasmid

    #35687

    Depositor

    Venkaiah Betapudi

    Article

    Dulyaninova et al Mol Biol Cell. 2007 Aug;18(8):3144-55. E

    Insert

    MHC IIA (MYH9 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-mTagBFP2-FRB-Rab5

    Plasmid

    #214272

    Purpose

    Mammalian expression of mTagBFP2-FRB-Rab5 (early endosome marker)

    Depositor

    Shinya Tsukiji

    Article

    Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

    Insert

    mTagBFP2-FRB-Rab5A (RAB5A Canis lupus familiaris (Dog))
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-miRFP670-eDHFR(69K6)

    Plasmid

    #178859

    Purpose

    Mammalian expression of miRFP670-eDHFR(69K6)

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    miRFP670-eDHFR(69K6)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV-miRFP2-IRES-HO1-N1

    Plasmid

    #178967

    Purpose

    Expresses miRFP2 and HO1 in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

    Insert

    miRFP2
    

    Use

    Tags

    HO1 and no

    Expression

    Mammalian

    Mutation

    NO

    Promoter

    CMV

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-mTagBFP2-FRB-Rab7

    Plasmid

    #214273

    Purpose

    Mammalian expression of mTagBFP2-FRB-Rab7 (late endosome marker)

    Depositor

    Shinya Tsukiji

    Article

    Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

    Insert

    mTagBFP2-FRB-Rab7A (RAB7A Canis lupus familiaris (Dog))
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV superYFP-AURKA-mTurq2

    Plasmid

    #157772

    Purpose

    Expression of AuroraA kinase biosensor under CMV promoter

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Use

    Tags

    mTurquoise2 and superYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pOTTC591 - pCMV Mem-iRFP

    Plasmid

    #59134

    Purpose

    A plasmid that express membrane-targeted iRFP under the CMV promoter.

    Depositor

    Brandon Harvey

    Article

    Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

    Insert

    Membrane-localized Infrared Fluorescent Protein
    

    Use

    Tags

    Palmitylation signal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-IE

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-RA-HRas-IRES2-Lyn-iRFP682

    Plasmid

    #180584

    Purpose

    RA-Hras and Lyn-iRFP682

    Depositor

    Won Do Heo

    Article

    Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

    Insert

    RA-Hras and Lyn-iRFP682
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-mScarlet-I-eDHFR-ORP5(ΔPH)

    Plasmid

    #214277

    Purpose

    Mammalian expression of ORP5(ΔPH) fused to mScarlet-I-tagged E. coli DHFR

    Depositor

    Shinya Tsukiji

    Article

    Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

    Insert

    mScarlet-I-eDHFR-ORP5(253-879) (OSBPL5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Polymerase expression - pCMV-Phi29 (LM2708)

    Plasmid

    #208965

    Purpose

    Unfused Phi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    Phi29-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    Phi29(-exo;D169A)

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Polymerase expression - pCMV-B103 (LM2907)

    Plasmid

    #208968

    Purpose

    Unfused B103 DNA polymerase, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    B103-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Polymerase expression - pCMV-T7-EcKlenow (LM2692)

    Plasmid

    #208963

    Purpose

    Unfused EcKlenow DNA polymerase (-exo), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    EcKlenow-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Polymerase expression - pCMV-ePhi29 (LM2802)

    Plasmid

    #208967

    Purpose

    Unfused ePhi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    ePhi29-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    ePhi29(-exo)(D169A;M8R/V51A/M97T/G197D/E221K/Q497…

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCMV superYFP-AURKA K162M-mTurq2

    Plasmid

    #157773

    Purpose

    Expression of AuroraA kinase-dead biosensor under CMV promoter

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Use

    Tags

    mTurquoise2 and superYFP

    Expression

    Mammalian

    Mutation

    AURKA K162M is a kinase-dead version of AURKA

    Promoter

    CMV

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Polymerase expression - pCMV-Phi29(+exo) (LM2759)

    Plasmid

    #208964

    Purpose

    Unfused Phi29 DNA polymerase (+exo), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    Phi29(+exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Recruited polymerase expression - pCMV-T7-EcKlenow-CC(N5) (LM2696)

    Plasmid

    #208969

    Purpose

    Recruited EcKlenow DNA polymerase (-exo) with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    EcKlenow-BPNLS-CC(N5)
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Recruited polymerase expression - pCMV-T7-B103-CC(N5) (LM2946)

    Plasmid

    #208974

    Purpose

    Recruited B103 DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    B103-BPNLS-CC(N5)
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Recruited polymerase expression - pCMV-T7-Phi29-CC(N5) (LM2726)

    Plasmid

    #208971

    Purpose

    Recruited Phi29 DNA polymerase (-exo) with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    Phi29-BPNLS-CC(N5)
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    Phi29(-exo;D169A)

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Polymerase expression - pCMV-ePhi29(+exo) (LM2995)

    Plasmid

    #208966

    Purpose

    Unfused ePhi29 DNA polymerase (+exo), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    ePhi29(+exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    ePhi29(+exo)(M8R/V51A/M97T/G197D/E221K/Q497P/K512…

    Promoter

    CMV and T7

    Available since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge