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Addgene

We narrowed to 594 results for: Ngf

Showing: 481 - 520 of 594 results
  1. pU6_B2M_sgRNA(PP7)

    Plasmid
    #232438
    Purpose
    sgRNA to facilitate a splice donor site disruption via adenine base editing in the B2M locus, contains a PP7 aptamer in the scaffold tetraloop
    Depositor
    Insert
    PP7-tagged gRNA for B2M disruption via splice donor consensus disruption via ABE8e or Cas9 driven by human U6 promoter
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    U6
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
  2. pUCM-CLYBL-NGN2-BRN3A

    Plasmid
    #165597
    Purpose
    Donor construct for insertion of NGN2 and BRN3A into the CLYBL safe harbor site. This is an all-in-one doxycycline-inducible construct, enabling iPSC differentiation into peripheral sensory neurons.
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    BRN3A (POU4F1 Human)
    EGFP
    Puromycin N-acetyl transferase
    rtTA3G
    SBP Delta-LNGFR
    Use
    CRISPR and TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG, EF-1alpha, Endogenous CLYBL (splice acceptor…
    Available since
    March 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  3. R777-E317 Hs.SOS1

    Plasmid
    #70601
    Purpose
    Gateway ORF clone of human SOS1 [NM_005633.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Insert
    SOS1 (SOS1 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
  4. pCGN-Sos Cat

    Plasmid
    #32917
    Depositor
    Insert
    SOS1 (SOS1 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Contains only aa 564-1049
    Promoter
    CMV
    Available since
    Jan. 31, 2012
    Availability
    Academic Institutions and Nonprofits only
  5. LentiGuide-10X-CS1-3'-puro

    Plasmid
    #212011
    Purpose
    Deliver gRNA through lentivirus (compatible with 10x capture Sequence 1)
    Depositor
    Insert
    gRNA scaffold
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 20, 2024
    Availability
    Academic Institutions and Nonprofits only
  6. R777-E318 Hs.SOS1-nostop

    Plasmid
    #70602
    Purpose
    Gateway ORF clone of human SOS1 [NM_005633.3] without stop codon (for C-terminal fusions)
    Depositor
    Insert
    SOS1 (SOS1 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
  7. mEgr2/LZRS

    Plasmid
    #27784
    Depositor
    Insert
    Egr2 (Egr2 Mouse)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 18, 2011
    Availability
    Academic Institutions and Nonprofits only
  8. NR4A1

    Plasmid
    #25477
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    NR4A1 (NR4A1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
  9. pNR4A1.1.0-gDNA

    Plasmid
    #112391
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor NR4A1
    Depositor
    Insert
    NR4A1 (NR4A1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
  10. pREST-donor

    Plasmid
    #113807
    Purpose
    CRISPR donor plasmid to tag human transcription factor REST with GFP
    Depositor
    Insert
    REST homology arms flanking EGFP-IRES-Neo cassette (REST Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    rs4864608
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
  11. pREST.1.0-gDNA

    Plasmid
    #113774
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor REST
    Depositor
    Insert
    REST (REST Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
  12. pNR4A1-donor

    Plasmid
    #112295
    Purpose
    CRISPR donor plasmid to tag human transcription factor NR4A1 with GFP
    Depositor
    Insert
    NR4A1 homology arms flanking EGFP-IRES-Neo cassette (NR4A1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:12:52058840:C>T, hg38:12:52058930:A>C,…
    Promoter
    Available since
    Nov. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
  13. AAVS1-DRGFP

    Plasmid
    #113193
    Purpose
    DR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.
    Depositor
    Insert
    DR-GFP
    Use
    Donor construct with t2a-hygromycin for gene trap…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
  14. pCDNA3-2xMARS-PLD

    Plasmid
    #205237
    Purpose
    Expresses two repeats of PLEKAH5 aa 143-271 (K163A and R164A) fused to phospholipase D from Streptomyces sp. PMF
    Depositor
    Inserts
    2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
    phospholipase D (PLD)
    Use
    Tags
    Nuclear Export Sequence and mCherry
    Expression
    Mammalian
    Mutation
    amino acids 143-271 of PLEKHA5 (GenBank reference…
    Promoter
    CMV
    Available since
    July 30, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 481 - 520 of 594 results