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We narrowed to 1,259 results for: naga

Showing: 481 - 520 of 1259 results

  6. pMRX-IP-EGFP-LOV2-OMP25

    Plasmid
    #233346
    Purpose
    Stable expression of EGFP-LOV2(N538E)-OMP25 in mammalian cells. This construct lacks BAX domain, and causes no rupture upon photostimulation.
    Depositor
    Noboru Mizushima
    Article
    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.
    Inserts
    LOV2 domain of phototropin1
    Omp25 (Synj2bp Rat)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N-terminus is deleted and N538E
    Promoter
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233346-plasmid-map-sequence-id-469428

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  7. pYI2294

    Plasmid
    #228352
    Purpose
    Methanol-inducible ALX-0081 expression
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
    Insert
    MFalpha(2xAdv)-LE-ALX-0081-3A-FLAG-His
    Use
    Affinity Reagent/ Antibody
    Tags
    AAA linker followed by FLAG-His6 and MF_ secretio…
    Expression
    Yeast
    Mutation
    Promoter
    KpAOX1 promoter
    Available since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228352-plasmid-map-sequence-id-457091

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  8. pYI2292

    Plasmid
    #228350
    Purpose
    Methanol-inducible ALX-0171 expression
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
    Insert
    MFalpha(2xAdv)-LE-ALX-0171-3A-FLAG-His
    Use
    Affinity Reagent/ Antibody
    Tags
    AAA linker followed by FLAG-His6 and MF_ secretio…
    Expression
    Yeast
    Mutation
    Promoter
    KpAOX1 promoter
    Available since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228350-plasmid-map-sequence-id-457095

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  9. pYI2293

    Plasmid
    #228351
    Purpose
    Constitutive ALX-0081 expression
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
    Insert
    MFalpha(2xAdv)-LE-ALX-0081-3A-FLAG-His
    Use
    Affinity Reagent/ Antibody
    Tags
    AAA linker followed by FLAG-His6 and MF_ secretio…
    Expression
    Yeast
    Mutation
    Promoter
    KpGAPDH promoter
    Available since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228351-plasmid-map-sequence-id-457078

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  10. pMT1461

    Plasmid
    #228319
    Purpose
    Doxycycline-regulatable ALX-0081 expression
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
    Insert
    MFalpha(2xAdv)-ALX-0081-3A-FLAG-His
    Use
    Affinity Reagent/ Antibody
    Tags
    AAA linker followed by FLAG-His6 and MF_ secretio…
    Expression
    Yeast
    Mutation
    Promoter
    Doxycycline-regulatable synthetic promoter (KpTc7…
    Available since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228319-plasmid-map-sequence-id-457683

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  11. p3R112C/T180CHFm

    Plasmid
    #85091
    Purpose
    Expresses PCNA3 variant (R112C/T180C) fused to heme-FMN domain of P450 BM3 variant (A74G) in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi
    Insert
    The R112C/T180C variant of PCNA3 fused to Heme-FMN domain of P450 BM3
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    R112C/T180C in PCNA3, A74G in heme-FMN domain of …
    Promoter
    T7 promoter
    Available since
    Dec. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    85091-plasmid-map-sequence-id-164485

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  13. pYI2165

    Plasmid
    #228347
    Purpose
    2,4-Diacetylphloroglucinol (DAPG)-regulatable ALX-0171 expression
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
    Insert
    MFalpha(2xAdv)-LE-ALX-0171-3A-FLAG-His
    Use
    Affinity Reagent/ Antibody
    Tags
    AAA linker followed by FLAG-His6 and MF_ secretio…
    Expression
    Yeast
    Mutation
    Promoter
    2,4-Diacetylphloroglucinol-regulatable synthetic …
    Available since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228347-plasmid-map-sequence-id-457108

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  14. pMRX-IP-EGFP-LOV2-Cyb5a

    Plasmid
    #233348
    Purpose
    Stable expression of EGFP-LOV2(N538E)-Cyb5a in mammalian cells. This construct lacks BAX domain, and causes no rupture upon photostimulation.
    Depositor
    Noboru Mizushima
    Article
    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.
    Inserts
    LOV2 domain of phototropin1
    Cyb5a (Cyb5a Rat)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N-terminus is deleted (Cyb5a 95-134) and N538E
    Promoter
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233348-plasmid-map-sequence-id-466824

    Enlarge

  18. pMRX-IP-EGFP-LOV2-TMEM106B

    Plasmid
    #233347
    Purpose
    Stable expression of EGFP-LOV2(G528A, N538E)-TMEM106B in mammalian cells. This construct lacks BAX domain, and causes no rupture upon photostimulation.
    Depositor
    Noboru Mizushima
    Article
    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.
    Inserts
    LOV2 domain of phototropin1
    TMEM106B (TMEM106B Human)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N-terminus is deleted (TMEM106B 90-274) and N538E
    Promoter
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233347-plasmid-map-sequence-id-469408

    Enlarge

  19. pYI2216

    Plasmid
    #228349
    Purpose
    2,4-Diacetylphloroglucinol (DAPG)-regulatable ALX-0081 expression
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
    Insert
    MFalpha(2xAdv)-LE-ALX-0081-3A-FLAG-His
    Use
    Affinity Reagent/ Antibody
    Tags
    AAA linker followed by FLAG-His6 and MF_ secretio…
    Expression
    Yeast
    Mutation
    Promoter
    2,4-Diacetylphloroglucinol-regulatable synthetic …
    Available since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228349-plasmid-map-sequence-id-457093

    Enlarge

  20. pATP416-­pluxO5-­pphlO6-­crtYBI

    Plasmid
    #165977
    Purpose
    Expresses carotenoid biosynthesis gene in response to homoserine lactone and 2,4-diacetylphloroglucinol in yeast expressing PhlTA and LuxTA
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:
    Inserts
    Xanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
    Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
    BTS1 (BTS1 Budding Yeast)
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    C1281A, G1698A and C66A, C1359T
    Promoter
    Synthetic promoter (pluxO5), Synthetic promoter (…
    Available since
    March 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    165977-plasmid-map-sequence-id-328856

    Enlarge

  21. pATP416-­ptetO7-­pphlO6-­crtYBI

    Plasmid
    #165976
    Purpose
    Expresses carotenoid biosynthesis gene in response to 2,4-diacetylphloroglucinol and doxycycline in yeast expressing rtTA and PhlTA
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:
    Inserts
    Xanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
    Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
    BTS1 (BTS1 Budding Yeast)
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    C1281A, G1698A and C66A, C1359T
    Promoter
    Synthetic promoter (pphlO6), Synthetic promoter (…
    Available since
    March 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    165976-plasmid-map-sequence-id-328850

    Enlarge

  22. pATP416-­pluxO5-­ptetO7-­crtYBI

    Plasmid
    #165975
    Purpose
    Expresses carotenoid biosynthesis gene in response to homoserine lactone and doxycycline in yeast expressing LuxTA and rtTA
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:
    Inserts
    Xanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
    Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
    BTS1 (BTS1 Budding Yeast)
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    C1281A, G1698A and C66A, C1359T
    Promoter
    Synthetic promoter (pluxO5), Synthetic promoter (…
    Available since
    March 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    165975-plasmid-map-sequence-id-328841

    Enlarge

  24. pMRX-IP-EGFP-PA-BAX(mito)

    Plasmid
    #228942
    Purpose
    Stable expression of EGFP-LOV2(N538E)-BAX-OMP25 in mammalian cells. Photoactivatable BAX localizes on the outer mitochondrial membrane and induces mitochondrial rupture upon blue light stimulation.
    Depositor
    Noboru Mizushima
    Article
    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.
    Inserts
    LOV2 domain of phototropin1
    BAX (BAX Human)
    Omp25 (Synj2bp Rat)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N-terminus and C-terminus are deleted. BAX(3-171)…
    Promoter
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228942-plasmid-map-sequence-id-466826

    Enlarge

  25. pMRX-IP-EGFP-PA-BAX(ER)

    Plasmid
    #228941
    Purpose
    Stable expression of EGFP-LOV2(N538E)-BAX-Cyb5a in mammalian cells. Photoactivatable BAX localizes on ER and induces ER rupture upon blue light stimulation.
    Depositor
    Noboru Mizushima
    Article
    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.
    Inserts
    LOV2 domain of phototropin1
    BAX (BAX Human)
    Cyb5a (Cyb5a Rat)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N-terminus and C-terminus are deleted. BAX(3-171)…
    Promoter
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228941-plasmid-map-sequence-id-469413

    Enlarge

  26. pMRX-IP-EGFP-PA-BAX(ER, G108V)

    Plasmid
    #233344
    Purpose
    Stable expression of EGFP-LOV2(N538E)-BAX(G108V)-Cyb5a in mammalian cells. The G108V mutation suppresses pore-forming activity of BAX.
    Depositor
    Noboru Mizushima
    Article
    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.
    Inserts
    LOV2 domain of phototropin1
    BAX (BAX Human)
    Cyb5a (Cyb5a Rat)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    BAX(3-171), N-terminus and C-terminus are deleted…
    Promoter
    Available since
    June 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    233344-plasmid-map-sequence-id-474567

    Enlarge

  27. pMRX-IP-EGFP-PA-BAX(lyso)

    Plasmid
    #228939
    Purpose
    Stable expression of EGFP-LOV2(G528A, N538E)-BAX-TMEM106B in mammalian cells. Photoactivatable BAX localizes on lysosomal membrane and induces lysosomal rupture upon blue light stimulation.
    Depositor
    Noboru Mizushima
    Article
    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.
    Inserts
    LOV2 domain of phototropin1
    BAX (BAX Human)
    TMEM106B (TMEM106B Human)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N-terminus and C-terminus are deleted. BAX(3-171)…
    Promoter
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228939-plasmid-map-sequence-id-469416

    Enlarge

  28. pMRX-IP-EGFP-PA-BAX(mito, G108V)

    Plasmid
    #233343
    Purpose
    Stable expression of EGFP-LOV2(N538E)-BAX(G108V)-OMP25 in mammalian cells. The G108V mutation suppresses pore-forming activity of BAX.
    Depositor
    Noboru Mizushima
    Article
    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.
    Inserts
    LOV2 domain of phototropin1
    BAX (BAX Human)
    Omp25 (Synj2bp Rat)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N-terminus and C-terminus are deleted. BAX(3-171)…
    Promoter
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233343-plasmid-map-sequence-id-469412

    Enlarge

  29. pMRX-IP-EGFP-PA-BAX(lyso, G108V)

    Plasmid
    #233345
    Purpose
    Stable expression of EGFP-LOV2(G528A, N538E)-BAX-TMEM106B in mammalian cells. The G108V mutation suppresses pore-forming activity of BAX.
    Depositor
    Noboru Mizushima
    Article
    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.
    Inserts
    LOV2 domain of phototropin1
    BAX (BAX Human)
    TMEM106B (TMEM106B Human)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    BAX(3-171), N-terminus and C-terminus are deleted…
    Promoter
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233345-plasmid-map-sequence-id-469414

    Enlarge

  30. pMT1219

    Plasmid
    #228301
    Purpose
    2,4-Diacetylphloroglucinol (DAPG)-regulatable expression of SARS-CoV-2 RBD omicron variant
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
    Insert
    MFalpha(2xAdv)-SARS CoV-2 receptor binding domain (RBD)-His (S Severe acute respiratory syndrome-related coronavirus 2, Synthetic)
    Use
    Synthetic Biology
    Tags
    GGG linker followed by His6 and MF_ secretion sig…
    Expression
    Yeast
    Mutation
    G339D, S371L, S373P, S375F, K417N, N440K, G446S, …
    Promoter
    2,4-Diacetylphloroglucinol-regulatable synthetic …
    Available since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228301-plasmid-map-sequence-id-457688

    Enlarge

Showing: 481 - 520 of 1259 results