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Plasmid#108886PurposeExpression of membrane localized mCherryDepositorInsertmCherry-CaaX(Hras)
UseCan be used for mammalian expression or in vitro …TagsExpressionMammalianMutationPromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
nls-mCherry
Plasmid#108881PurposeExpression of nuclear localized mCherryDepositorInsertnls-mCherry
UseCan be used for mammalian expression or in vitro …TagsExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTRE-tdTomato
Plasmid#50798PurposeExpression of tdTomato under the control of the TRE-promoterDepositorInsertTREtight driven tdTomato fluorescent protein
UseMutli-speciesTagsExpressionMutationPromoterTREtightAvailable sinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHR_EF1a-->myc-LaG17-tTA synNotch (LA1)
Plasmid#162230PurposeLentiviral vector for the expression of GFP nanobody LaG17 tTA SynNotch: pHR_EF1a-->myc-LaG17-tTA synNotch (LA1) in mammalian cellsDepositorInsertLaG17 GFP nanobody SynNotch tTA
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNIR-FbGFP
Plasmid#184675PurposeNIR-Fb to GFPDepositorInsertNIR-FbGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
H2B-mCherry
Plasmid#108880PurposeExpression of human histone2B fused to mCherry (nuclear marker)DepositorInsertH2B-mCherry
UseCan be used for mammalian expression or in vitro …TagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBTSG
Plasmid#159420PurposeTransient expression of proteins with a C-terminal thermostable GFP (TGP) tag in mammalian cellsDepositorInsertthermostable GFP (TGP)
UseTagspolyhistidine (poly-His)ExpressionMammalianMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUAS-Cre
Plasmid#50797PurposeExpression of Cre recombinase under the control of the UAS-promoterDepositorInsertCre recombinase
UseMulti-speciesTagsExpressionMutationPromoterUAS-hsp70Available sinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pETSG
Plasmid#159418PurposeExpression of proteins with a C-terminal thermostable GFP (TGP) tag in Escherichia coliDepositorInsertthermostable GFP (TGP)
UseTagspolyhistidine (poly-His)ExpressionBacterialMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNIR-Fbactin
Plasmid#184677PurposeNIR-Fb to actinDepositorInsertNIR-Fbactin
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Flp-DOG
Plasmid#80084PurposeFlp recombinase dependent on GFP (Flp-DOG) for expression in mammalian cellsDepositorInsertFlp-DOG
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS2+_NLS-Tir1-HA
Plasmid#117716PurposemRNA synthesis of NLS-TIR1-HA for injectionDepositorInsertTRANSPORT INHIBITOR RESPONSE 1
UseTagsHA and NLSExpressionMammalianMutationcodon-optimized TIR1 for murine expressionPromoterSP6Available sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOPINVHH_BAP_his
Plasmid#210402PurposeFor expression of VHH with C-terminal biotin acceptor peptide and his tagDepositorTypeEmpty backboneUseTagsbiotin acceptor peptide-his6ExpressionBacterialMutationPromoterlacAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINVHH_his
Plasmid#210405PurposeFor expression of VHH with C-terminal his tagDepositorTypeEmpty backboneUseTagshis6ExpressionBacterialMutationPromoterlacAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiex-1 Myosin VI HMM_eGFP
Plasmid#82714PurposeExpresses Myosin VI HMM with a C-terminal eGFP.DepositorInsertMyosin VI
UseTagsFLAG purification tag and eGFPExpressionBacterial and InsectMutationPromoterT7 PromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-2xMARS-nGFP-puro
Plasmid#205239PurposeDOX inducible expression of two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells from the Sleeping Beauty transposon systemDepositorInsert2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
UseTransposonTagsHA, Nuclear Export Sequence, mScarlet-i, and nGFP…ExpressionMammalianMutationamino acids 143-271 of PLEKHA5 (GenBank reference…Promotertight TRE promoterAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNIR-FbmCherry
Plasmid#184676PurposeNIR-Fb to mCherryDepositorInsertNIR-FbmCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
BbZIP-cALFA
Plasmid#160498PurposeFor the protein expression of BbZIP with ALFA tag at C-terminus in E. coliDepositorInsertBbZIP
UseTagsHRV3C protease cleavage site-ALFA tag-8histidineExpressionBacterialMutationPromoterAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDX_init containing Sb-loop
Plasmid#132697PurposepDX_init plasmid containing a loop Sybody for the generation of a qPCR standard curveDepositorInsertSb_loop
UseM13 phagmid vectorTagsExpressionMutationPromoterlacAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GFPmG1
Plasmid#50444PurposeExpression of a EGFP variant in mammalian cellsDepositorInsertGFPmG1
UseTagsExpressionMammalianMutationE143K, N147QPromoterCAGAvailable sinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pME-EGFP-P2A-APEX2-NS
Plasmid#108895PurposeMultisite gateway vector for 5' tagging with APEX2 (includes cytoplasmic GFP reporter)DepositorInsertEGFP-P2A-APEX2
UseMultisite gatewayTagsExpressionMutationPromoterAvailable sinceMay 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOPINVHH_cys_his
Plasmid#210403PurposeFor expression of VHH with C-terminal cysteine and his tagDepositorTypeEmpty backboneUseTagscys-his6ExpressionBacterialMutationPromoterlacAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINVHH_his_flag
Plasmid#210404PurposeFor expression of VHH with C-terminal his and Flag tagsDepositorTypeEmpty backboneUseTagshis6-flagExpressionBacterialMutationPromoterlacAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINVHH_sortase_his
Plasmid#210406PurposeFor expression of VHH with C-terminal sortase and his tagsDepositorTypeEmpty backboneUseTagssortase-his6ExpressionBacterialMutationPromoterlacAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINVHH_snap_his
Plasmid#210407PurposeFor expression of VHH with C-terminal snap protein and his tagDepositorTypeEmpty backboneUseTagsHis6ExpressionBacterialMutationPromoterlacAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINVHH_his_myc
Plasmid#210526PurposeFor expression of VHH with C-terminal his and myc tagsDepositorTypeEmpty backboneUseTagsHis6-mycExpressionBacterialMutationPromoterlacAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pB2AR-miRFP670nano3
Plasmid#184671PurposeBeta-2-adrenergic receptor internally tagged with miRFP670nano3DepositorInsertB2AR-miRFP670nano3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYTSG
Plasmid#159419PurposeExpression of proteins with a C-terminal thermostable GFP (TGP) tag in Saccharomyces cerevisiaeDepositorInsertthermostable GFP (TGP)
UseTagspolyhistidine (poly-His)ExpressionYeastMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNIR-Fb2E7
Plasmid#184681PurposeNIR-Fb to HIV gp41DepositorInsertNIR-Fb2E7
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTSG
Plasmid#162389PurposeExpression of proteins with a C-terminal thermostable GFP (TGP) tag in insect cellDepositorInsertthermostable GFP (TGP)
UseTagsHisExpressionInsectMutationPromoterAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNIR-Fb4EIG
Plasmid#184683PurposeNIR-Fb to E.coli dihydrofolate reductaseDepositorInsertNIR-Fb4EIG
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNIR-Fb59H10
Plasmid#184682PurposeNIR-Fb to HIV p24DepositorInsertNIR-Fb59H10
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTrcHis-7D12Gly42TAG
Plasmid#125265PurposeExpresses 7D12 anti-EGFR nanobodies with UAG codon at Gly42 for synthetic amino acid in combination with pEVOL-pAzFDepositorInsert7D12 Gly42 nanobody
UseTagsHis-tagExpressionBacterialMutationPromotertrc promoterAvailable sinceMay 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCOLA-Gent-ppiB
Plasmid#202482PurposeExpresses peptidyl-prolyl cis-trans isomerase (ppiB) after phosphae depletionDepositorInsertpeptidyl-prolyl cis-trans isomerase (ppiB)
UseTagsExpressionBacterialMutationPromoteryibDpAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETM6-Amp-T7-GFPuv
Plasmid#202463PurposeExpresses GFPuv with T7 inductionDepositorInsertGFPuv
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-LCAmp-trxB
Plasmid#202465PurposeExpresses a guide RNA to silence trxB after phosphate depletionDepositorInserttrxB guide RNA
UseTagsExpressionBacterialMutationPromoterugpBAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-sfGFP(1-157)
Plasmid#220743PurposeGAL4-sfGFP(1-157) construct expression in mammlian cells.DepositorInsertGAL4-sfGFP(1-157)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-sfGFP(158-238)
Plasmid#220744PurposeGAL4-sfGFP(158-238) construct expression in mammlian cells.DepositorInsertGAL4-sfGFP(158-238)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-NIR-FbLAG30
Plasmid#220746PurposeGAL4-NIR-FbLAG30 construct expression in mammlian cells.DepositorInsertGAL4-NIR-FbLAG30
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNIR-FbLAG16
Plasmid#220739PurposeNIR-FbLAG16 expression in mammalian cells.DepositorInsertNIR-FbLAG16
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only