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Showing: 601 - 650 of 2294 results

  2. PET28-FnCas12a-EP16

    Plasmid
    #183636
    Purpose
    PET28-FnCas12a-EP16 can efficiently recognize a broad range of PAM sequences including YN (Y = C or T), TAC and CAA.
    Depositor
    Yunzi Luo
    Article
    Wang et al Biotechnol Bioeng. 2019 Jun;116(6):1463-
    Insert
    FnCas12a-EP16
    Use
    CRISPR
    Tags
    6×His Tag
    Expression
    Bacterial
    Mutation
    N607R, K613V, N617R, K180S, K660R, D616N
    Promoter
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    183636-plasmid-map-sequence-id-375575

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  25. pET28-his-3C_Clu3B6p

    Plasmid
    #100093
    Purpose
    expresses easily crystallizable engineered 14-3-3 sigma variant fused to the HSPB6 phosphorylatable peptide
    Depositor
    Nikolai Sluchanko
    Article
    Sluchanko et al Sci Rep. 2017 Sep 20;7(1):12014. doi: 10
    Insert
    14-3-3 sigma human (SFN Human)
    Use
    Tags
    HSPB6 peptide and His6
    Expression
    Bacterial
    Mutation
    75-77 residues changed to AAA to facilitate cryst…
    Promoter
    T7
    Available since
    Sept. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    100093-plasmid-map-sequence-id-193872

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  32. pET28(a)-hFX(I154F)-HA

    Plasmid
    #14992
    Depositor
    Michael Ristow
    Insert
    human frataxin cDNA (FXN Human)
    Use
    Tags
    AU1, HA, and HIS
    Expression
    Bacterial
    Mutation
    contains cDNA coding sequence for amino acids 88-…
    Promoter
    Available since
    Aug. 17, 2007
    Availability
    Academic Institutions and Nonprofits only

  33. pET28a-RANGAP1-CTD

    Plasmid
    #228947
    Purpose
    express the C-terminal domain of the human RANGAP1 protein (residues 418-587 of UniProt P46060 with the N-terminal thrombin-cleavable MGSSHHHHHHSSGLVPR|GSHM- tag). Codon-optimized
    Depositor
    Marcin Suskiewicz
    Insert
    RANGAP1-CTD (RANGAP1 Human)
    Use
    Tags
    His tag and thrombine cleavage site : MGSSHHHHHHS…
    Expression
    Bacterial
    Mutation
    contains aa 418-587 only
    Promoter
    T7-lac
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    228947-plasmid-map-sequence-id-472386

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  46. pET28-NFHΔ462-638

    Plasmid
    #232061
    Purpose
    Expresses construct for surface tethering: R. norvegicus NFH tail domain lacking residues 462-638
    Depositor
    Sanjay Kumar
    Article
    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121
    Insert
    Neurofilament-Heavy tail domain lacking residues 462-638
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    added N-terminal tryptophan and tyrosine, and cys…
    Promoter
    Available since
    March 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    232061-plasmid-map-sequence-id-464068

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  50. pET28b-SUMO-SeKaiA

    Plasmid
    #229218
    Purpose
    For expression of S. elongatus KaiA protein
    Depositor
    Andy LiWang
    Insert
    kaiA (kaiA S. elongatus)
    Use
    Tags
    FLAG and SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    229218-plasmid-map-sequence-id-457045

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Showing: 601 - 650 of 2294 results