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actc1b-mKate2-ING2

Plasmid

#109502

Purpose

Muscle specific zebrafish expression of mKate2 fused to 3xPHD domain from Mouse inhibitor of growth family member 2 (ING2), codon optimised. Binds PtdIns5P

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

actc1b-mKate2-ING2

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_NR1D1_Hormone-recep

Plasmid

#109960

Purpose

Protein expression and purification of NR1D1_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

NR1D1_Hormone-recep

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pSB1C3-H3

Plasmid

#109381

Purpose

Arabinose inducible VioB-mCherry expression (weaker RBS). Used to test burden response in E. coli

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Insert

pARABAD-VioB-mCherry

Expression

Bacterial

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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CLXSN 5myc-CDC6-mut

Plasmid

#109333

Purpose

express tagged Cdc6 mutant

Depositor

Jean Cook

Article

Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

Insert

CDC6 (CDC6 Human)

Use

Retroviral

Tags

5x Myc

Expression

Mammalian

Mutation

R56A, L59A, K81A, E82A, N83A

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pTHSSe_51

Plasmid

#109249

Purpose

p15A BbsI cloning vector

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Expression

Bacterial

Promoter

PJ23105

Available Since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pDJSVT91

Plasmid

#109305

Purpose

Express periplasmic, outer membrane, or secreted proteins in E. coli - SufI signal (TAT) and His6 tag

Depositor

Daniel Slade

Article

Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

Type

Empty backbone

Tags

SufI 1-30, 6His

Expression

Bacterial

Promoter

T7

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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pKM490-Clip

Plasmid

#109281

Purpose

ORBIT integrating plasmid for C-terminal tagging with CLIP tag

Depositor

Kenan Murphy

Article

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Insert

attB-CLIP tag

Use

Orbit integrating plasmid

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1hisA cdc25c

Plasmid

#10964

Depositor

Toren Finkel

Article

Savitsky et al J Biol Chem. 2002 Jun 7. 277(23):20535-4

Insert

cdc25c (CDC25C Human)

Tags

6xHis

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pCAG-C1V1-TT-mRuby2-ST

Plasmid

#109124

Purpose

Cation channelrhodopsin C1V1 fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

C1V1-TT-mRuby2-ST

Expression

Mammalian

Promoter

CAG

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-GFP-ATL1

Plasmid

#109018

Purpose

stable lentiviral expression of cDNA

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL1 (ATL1 Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Promoter

EIF-1a promoter

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_LASP1_LIM

Plasmid

#109780

Purpose

Protein expression and purification of LASP1_LIM

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

LASP1_LIM (LASP1 Human)

Expression

Bacterial

Available Since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_RAB5A_Ras

Plasmid

#109935

Purpose

Protein expression and purification of RAB5A_Ras

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

RAB5A_Ras

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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GST cdc25c

Plasmid

#10969

Depositor

Toren Finkel

Article

Savitsky et al J Biol Chem. 2002 Jun 7. 277(23):20535-4

Insert

cdc25c (CDC25C Human)

Tags

GST

Expression

Bacterial

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only
pDS-Red1-N1 AMPA GluR-B(flip)

Plasmid

#109142

Purpose

The expression vector carries the red fluorescent protein tagged at the C-terminus of GluA2 subunit flip spliced form of the AMPA receptor.

Depositor

Stefano Vicini

Insert

AMPA receptor

Tags

RFP

Expression

Mammalian

Mutation

Glua2 subunit flip spliced form of the AMPA recep…

Promoter

CMV

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-ChETA-mRuby2-ST

Plasmid

#109126

Purpose

Cation channelrhodopsin ChETA fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

ChETA-mRuby2-ST

Expression

Mammalian

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pEXL flag Smad3

Plasmid

#10920

Depositor

Bob Weinberg

Article

Liu et al Proc Natl Acad Sci U S A. 1997 Sep 30. 9

Insert

Smad3 (SMAD3 Human)

Tags

flag

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pHH0103_GNA15_G-alpha

Plasmid

#109782

Purpose

Protein expression and purification of GNA15_G-alpha

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

GNA15_G-alpha (GNA15 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b- mKate2-KDEL-pA

Plasmid

#109494

Purpose

Muscle specific zebrafish expression of mKate2 fused to the 5' signal sequence from calreticulin and 3' KDEL ER retention sequence

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

actc1b- mKate2-KDEL-pA

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-rab27a

Plasmid

#109617

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab27a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab27a (rab27a Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-LbCpf1-U6_FlipArray

Plasmid

#109349

Purpose

Lentiviral vector to express Cpf1 from EFS promoter, as well as a Cpf1 FlipArray from U6 promoter.

Depositor

Sidi Chen

Article

Chow et al Nat Commun. 2018 May 15;9(1):1903. doi:

Insert

U6-FlipArray

Use

CRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…

Expression

Mammalian

Promoter

U6

Available Since

July 14, 2018

Availability

Academic Institutions and Nonprofits only

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