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Addgene
Showing: 241 - 260 of 17278 results
  1. pGL3 basic Mut E2

    Plasmid
    #48748
    Purpose
    Expression of luciferase driven by mPer2 promoter fragment containing mutated E-box2 (bases -112 to +98 with respect to transcription start site at +1)
    Depositor
    Insert
    mPer2 promoter/enhancer region containing mutated E-box2 (-112 to +98)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Mutated E-box2 sequence from CACGTT to GCTAGT
    Promoter
    Available since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
  2. pmIL-6 mut AP-1+C/EBP

    Plasmid
    #61292
    Purpose
    drives luciferase from mouse IL-6 promoter with mutations in both AP-1 & C/EBP sites
    Depositor
    Insert
    IL-6 promoter (Il6 Mouse)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Mutated both AP-1 binding site from TGAGTCT to TG…
    Promoter
    IL-6 promoter with mutant AP-1 and C/EBP binding …
    Available since
    May 4, 2015
    Availability
    Academic Institutions and Nonprofits only
  3. pAAV-hSyn-GRAB_NEmut

    Plasmid
    #123310
    Purpose
    Expresses the genetically-encoded fluorescent norepinephrine(NE) control sensor GRAB_NEmut in neurons
    Depositor
    Has Service
    AAV Retrograde and AAV9
    Insert
    GPCR activation based NE control sensor GRAB_NEmut
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    hSyn
    Available since
    April 4, 2019
    Availability
    Academic Institutions and Nonprofits only
  4. pINDUCER dCas9-TET1CDMut

    Plasmid
    #101920
    Purpose
    The dCas9-TET1CDMut fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948). The TET1 catalytic domain is mutated to abolish catalytic function.
    Depositor
    Insert
    dCas9-TET1CD Mut
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Mutated TET1 catalytic domain
    Promoter
    Available since
    Nov. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
  5. pcDNA3-CBX1mut-PA-mCherry

    Plasmid
    #138251
    Purpose
    Triple-mutant CBX1 chromodomain (residues 20-73) tagged with photoactivatable mCherry (PAmCherry). Mutations V3E/K6E/D40S correspond to positions V22/K25/D59 in full-length CBX1.
    Depositor
    Insert
    CBX1mut-PAmCherry
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 30, 2020
    Availability
    Academic Institutions and Nonprofits only
  6. pET-APOBEC1-YTHmut

    Plasmid
    #200159
    Purpose
    For bacterial expression of the APOBEC1-YTHmut fusion protein for protein purification
    Depositor
    Insert
    APOBEC1-YTHmut
    Use
    Tags
    6xHis, HA, and MBP
    Expression
    Bacterial
    Mutation
    Codon optimized for E coli expression
    Promoter
    LacZ
    Available since
    June 9, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 241 - 260 of 17278 results