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Str-STIM1-NN_ST-SBP-EGFP

Plasmid

#65262

Purpose

synchronize trafficking of ST from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Str-STIM1-NN and truncated sialyltransferase (ST6GAL1 Human)

Use

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Mutation

contains only amino acids 1 to 43 of ST

Promoter

CMV

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-MBP-HsStaufen1-T3_V

Plasmid

#147744

Purpose

Mammalian Expression of HsStaufen1-T3

Depositor

Elisa Izaurralde

Insert

HsStaufen1-T3 (STAU1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-MBP-HsStaufen1-T2_V

Plasmid

#147743

Purpose

Mammalian Expression of HsStaufen1-T2

Depositor

Elisa Izaurralde

Insert

HsStaufen1-T2 (STAU1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pStr-KDEL_TPV-CXCL9-mCherry-SBP

Plasmid

#170677

Purpose

Expresses ER-targeted streptavidin-KDEL (hook) and CXCL9-mCherry-SBP targeted to the ER lumen with an N-terminal TPV tripeptide (cargo) for RUSH.

Depositor

Ke Xu

Article

Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

Inserts

CXCL9 (CXCL9 Human, Synthetic)
streptavidin-KDEL

Use

Tags

SBP and mCherry

Expression

Mammalian

Mutation

Promoter

IRES and T7

Available since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO/FLAG-HA-SBP-TNRC6A

Plasmid

#42044

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Use

Tags

FLAG, HA, and SBP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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Str-STIM1-NN_ST-SBP-mCherry

Plasmid

#65263

Purpose

synchronize trafficking of ST from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Str-STIM1-NN and truncated sialyltransferase (ST6GAL1 Human)

Use

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Mutation

contains only amino acids 1 to 43 of ST

Promoter

CMV

Available since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-MBP-HsStaufen2-T1_V

Plasmid

#147745

Purpose

Mammalian Expression of HsStaufen2-T1

Depositor

Elisa Izaurralde

Insert

HsStaufen2-T1 (STAU2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRS406-PHO5-GFP-OSBP PH domain

Plasmid

#58840

Purpose

Expresses GFP-Tagged OSBP PH domain in yeast

Depositor

Sean Munro, Tim Levine

Article

Levine et al Curr Biol. 1998 Jun 18;8(13):729-39.

Insert

Oxysterol-binding Protein PH domain (OSBP Human)

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

PHO5

Available since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only
pT7-V5-SBP-C1-HsUpf1_1-925-siRNAres_P

Plasmid

#147191

Purpose

Mammalian Expression of HsUpf1_1-925-siRNAres

Depositor

Elisa Izaurralde

Insert

HsUpf1_1-925-siRNAres (UPF1 Human)

Use

Tags

Expression

Mammalian

Mutation

4 non silent mutations A69S, E167G, K282R and A4…

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HsUpf1-del296-926_P

Plasmid

#147195

Purpose

Mammalian Expression of HsUpf1-del296-926

Depositor

Elisa Izaurralde

Insert

HsUpf1-del296-926 (UPF1 Human)

Use

Tags

Expression

Mammalian

Mutation

4 non silent mutations A69S, E167G, K282R and T1…

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HsUpf1_115-1129-siRNAres_P

Plasmid

#147193

Purpose

Mammalian Expression of HsUpf1_115-1129-siRNAres

Depositor

Elisa Izaurralde

Insert

HsUpf1_115-1129-siRNAres (UPF1 Human)

Use

Tags

Expression

Mammalian

Mutation

5 non silent mutations A69S, E167G, K282R, A442G…

Promoter

Available since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-MBP-HsNot4_64-575_AG

Plasmid

#148766

Purpose

Mammalian Expression of HsNot4_64-575

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_64-575 (CNOT4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FLAG-SBP-STAG2 (S97X)

Plasmid

#73964

Purpose

STAG2 cDNA (CCDS43990) S97X

Depositor

Todd Waldman

Article

Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

Insert

STAG2 (STAG2 Human)

Use

Tags

FLAG-SBP

Expression

Mammalian

Mutation

S97X

Promoter

Available since

March 7, 2016

Availability

Academic Institutions and Nonprofits only
pVLAD6-mmPtch1-B-NNQ-TEV-SBP

Plasmid

#120887

Purpose

BacMam vector for Ptch1-B NNQ variant expression

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

Ptch1 (Ptch1 Mouse)

Use

Tags

SBP

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291, N…

Promoter

CMV

Available since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FLAG-SBP-STAG2 (S1215X)

Plasmid

#73970

Purpose

STAG2 cDNA (CCDS43990) S1215X

Depositor

Todd Waldman

Article

Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

Insert

STAG2 (STAG2 Human)

Use

Tags

FLAG-SBP

Expression

Mammalian

Mutation

S1215X

Promoter

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-FLAG-SBP-STAG2 (Q1117X)

Plasmid

#73968

Purpose

STAG2 cDNA (CCDS43990) Q1117X

Depositor

Todd Waldman

Article

Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

Insert

STAG2 (STAG2 Human)

Use

Tags

FLAG-SBP

Expression

Mammalian

Mutation

Q1117X

Promoter

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-FLAG-SBP-STAG2 (S1075X)

Plasmid

#73967

Purpose

STAG2 cDNA (CCDS43990) S1075X

Depositor

Todd Waldman

Article

Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

Insert

STAG2 (STAG2 Human)

Use

Tags

FLAG-SBP

Expression

Mammalian

Mutation

S1075X

Promoter

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-FLAG-SBP-STAG2 (S653X)

Plasmid

#73965

Purpose

STAG2 cDNA (CCDS43990) S653X

Depositor

Todd Waldman

Article

Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

Insert

STAG2 (STAG2 Human)

Use

Tags

FLAG-SBP

Expression

Mammalian

Mutation

S653X

Promoter

Available since

March 7, 2016

Availability

Academic Institutions and Nonprofits only
Str-KDEL_SBP-EGFP-CCR5-Cys3A

Plasmid

#222313

Purpose

Synchronize the trafficking of CCR5-Cys3A from the ER.

Depositor

Franck Perez

Article

Boncompain et al Sci Adv. 2019 Oct 16;5(10):eaax0821. doi

Insert

Streptavidin-KDEL and CCR5-Cys3A fused to SBP-EGFP (CCR5 Human)

Use

Tags

Expression

Mammalian

Mutation

C321A, C323A, C324A

Promoter

CMV

Available since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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Str-KDEL-SBP-EGFP-LAMP1

Plasmid

#222317

Purpose

Synchronize the trafficking of LAMP1 from the ER.

Depositor

Franck Perez

Article

Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

Insert

Streptavidin-KDEL and LAMP1 fused to SBP-EGFP (LAMP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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