We narrowed to 494 results for: blasticidin resistance

1. lentiBE3-SpRY-Blast

   Plasmid

   #199303

   Purpose

   Expresses SpRY Cas9 nickase cytosine base editor FNLS-BE3 and blasticidin resistance

   Depositor

   Neville Sanjana

   Article

   Morris et al Science. 2023 May 19;380(6646):eadh7699.

   Insert

   FNLS-BE3-SpRY
   

   Use

   CRISPR and Lentiviral

   Tags

   2A tag

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSH-EFIRES-B-AtAFB2-mCherry

   Plasmid

   #129718

   Purpose

   AAVS1 safe harbor targeting vector with blasticidin resistance gene expressing AtAFB2-mCherry

   Depositor

   Elina Ikonen

   Article

   Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

   Insert

   AFB2 (AFB2 Mustard Weed)
   

   Use

   CRISPR and TALEN; Human safe harbor locus (a avs1…

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   EF1a

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. lentiCRISPRi(v1)-Blast

   Plasmid

   #170067

   Purpose

   Expresses dCas9 repressor KRAB-dCas9 and blasticidin resistance

   Depositor

   Neville Sanjana

   Article

   Morris et al Science. 2023 May 19;380(6646):eadh7699.

   Insert

   KRAB-dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   2A and 2xHA tag

   Expression

   Mammalian

   Promoter

   EFS-NS

   Available Since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti_Cas9_T2A_mNeonGreen_P2A_blasticidin

   Plasmid

   #211471

   Purpose

   lentiviral tricistronic complex expressing Cas9, mNeonGreen, and blasticidin resistance

   Depositor

   James Eshleman

   Article

   Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

   Insert

   Cas9_T2A_mNeonGreen_P2A_BlasticidinR
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFlag

   Expression

   Mammalian

   Promoter

   hU6, EF-1a

   Available Since

   March 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. SpCas9-NG-APOBEC-nCas9-Ung

   Plasmid

   #198539

   Purpose

   Expresses human codon-optimized SpCas9-NG-APOBEC-nCas9-Ung and blasticidin resistance: EFS promoter-SpCas9-NG-APOBEC-nCas9-Ung-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NG-APOBEC-nCas9-Ung
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCKC764

   Plasmid

   #226625

   Purpose

   piggyBAC pCAG-hCas9-T2A-Unbiased3

   Depositor

   Chang Liu

   Article

   Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

   Inserts

   CAG promoter, SpCas9, T2A, Unbiased3 variant of TdT (DNTT S. pyogenes, Human, Chicken)
   EF1a promoter, blasticidin resistance
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV and EF1a

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. SpCas9-NRCH-ABE8.17-m+V106W

   Plasmid

   #198551

   Purpose

   Expresses human codon-optimized SpCas9-NRCH-ABE8.17-m+V106W and blasticidin resistance: EFS promoter-SpCas9-NRCH-ABE8.17-m+V106W-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NRCH-ABE8.17-m+V106W
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. BlastR-P2A-DD-TCOF1-donor

   Plasmid

   #60748

   Purpose

   Donor plasmid for HR-mediated recombination to insert blasticidin resistance and append a destabilization domain to the N-terminus of TCOF1

   Depositor

   Benhur Lee

   Article

   Park et al PLoS One. 2014 Apr 17;9(4):e95101. doi:

   Insert

   TCOF1 (TCOF1 Human)
   

   Use

   Targeting donor

   Available Since

   Oct. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

9. SpCas9-NRCH-APOBEC-nCas9-Ung

   Plasmid

   #198552

   Purpose

   Expresses human codon-optimized SpCas9-NRCH-APOBEC-nCas9-Ung and blasticidin resistance: EFS promoter-SpCas9-NRCH-APOBEC-nCas9-Ung-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NRCH-APOBEC-nCas9-Ung
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLex_Cas9

    Plasmid

    #117987

    Purpose

    pLex_Cas9 is a single insert lentiviral vector expressing Cas9 driven by CMV promoter.

    Depositor

    Paul Khavari

    Article

    Rubin et al Cell. 2018 Dec 11. pii: S0092-8674(18)31

    Insert

    Cas9-P2A-NLS-BASTR
    

    Use

    Lentiviral

    Tags

    P2A linker, nuclear localization signal and blast…

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. VRQR-HF1 variant

    Plasmid

    #138563

    Purpose

    Expresses human codon-optimized SpCas9-VRQR-HF1 and blasticidin resistance: EFS promoter-VRQR-HF1-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    VRQR-HF1
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…

    Promoter

    EFS

    Available Since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pWZLblasti-HA-MEK1

    Plasmid

    #53161

    Purpose

    Expresses HA tagged human MEK1 from blasticidin resistance retroviral vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    MEK1 (MAP2K1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Available Since

    May 28, 2014

    Availability

    Academic Institutions and Nonprofits only

13. BE3-NLS-NLS-P2A-BLAST

    Plasmid

    #135348

    Purpose

    Expression of BE3 (Base editor generation three) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    BE3-2XNLS
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pWZLblasti-HA-MEK1 C121S

    Plasmid

    #53165

    Purpose

    Expresses HA tagged human MEK1 C121S from blasticidin resistance retroviral vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    MEK1 (MAP2K1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C121S

    Available Since

    June 24, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pCAG-AviMta3-3xFiBsd

    Plasmid

    #140962

    Purpose

    full-length wt Avi-Mta3-3XFLAG-iBsd cDNA w/CAG promoter and Blasticidin resistance, cloned from mouse ES cells

    Depositor

    Brian Hendrich

    Article

    Burgold et al EMBO J. 2019 Jun 17;38(12). pii: embj.20

    Insert

    metastasis associated 3 (Mta3 Mouse, Synthetic)
    

    Tags

    3xFLAG, Avi, and TEV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pWZLblasti-HA-MEK5

    Plasmid

    #53167

    Purpose

    Expresses HA tagged human MEK5 from blasticidin resistance retroviral vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    MEK5 (MAP2K5 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Available Since

    June 24, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pCMV-ABEMAX-NLS-P2A_BLAST

    Plasmid

    #135355

    Purpose

    Expression of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistance

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    ABEMax-2XNLS
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pWZLblasti-HA-MEK1-MEK5DD

    Plasmid

    #53177

    Purpose

    Expresses HA tagged chimeric human MEK1 (nucleotides 1–201)-MEK5 (498–1344 with S311DT315D) from blasticidin resistance retroviral vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    MEK1 (1-201)-MEK5 (498–1344) fusion (MAP2K1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    MEK1 (1-201) MEK5(498–1344 with S311D/T315D)

    Available Since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

19. TRE3G-ZIM3-Cas9-P2A-GFP-PGK-Blasti

    Plasmid

    #239604

    Purpose

    Doxycycline inducible CRISPRgenee construct with a blasticidin resistance

    Depositor

    Philipp Rathert

    Article

    Stadager et al Cell Rep Methods. 2025 Jun 16;5(6):10107

    Insert

    ZIM3 KRAB domain (ZIM3 )
    

    Use

    CRISPR and Lentiviral

    Promoter

    TRE3G

    Available Since

    Sept. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. EF1a-ZIM3-Cas9-P2A-GFP-PGK-Blasti

    Plasmid

    #239610

    Purpose

    Constitutive active CRISPRgenee construct with a blasticidin resistance

    Depositor

    Philipp Rathert

    Article

    Stadager et al Cell Rep Methods. 2025 Jun 16;5(6):10107

    Insert

    ZIM3 KRAB domain (ZIM3 )
    

    Use

    CRISPR and Lentiviral

    Available Since

    Sept. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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