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pTRE-TIGHT-EGFP-donor fw copy

Plasmid

#22074

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

Insert

TETO-eGFP

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 28, 2009

Availability

Academic Institutions and Nonprofits only

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TALED_Right-mouse-ATP6-1397C-AD(V28R)

Plasmid

#212955

Purpose

Expresses TALED_Right-mouse-ATP6-1397C-AD(V28R) in mammalian cells

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2024 Jan 4;187(1):95-109.e26. doi:

Insert

Right-mouse-ATP6-1397C-AD(V28R) (ATP6 Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-GAL4-iLight-VP16

Plasmid

#170274

Purpose

NLS-Gal4(1-65)-iLight(human codon optimized)-VP16 construct expression in mammalian cells. pAAV vector.

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

Insert

NLS-Gal4(1-65)-iLight(human codon optimized)-VP16

Use

AAV

Tags

Expression

Mutation

See Comments

Promoter

CaMKII

Available since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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pLEVI(408)-ColE-iLight-msfGFP

Plasmid

#170268

Purpose

Expresses LexA_DBD-iLight-msfGFP under J23116 promoter and mCherry under ColE promoter with a LexA408 operator in bacteria.

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

Insert

iLight

Use

Tags

msfGFP

Expression

Bacterial

Mutation

To design iLight for expression in bacteria, a fu…

Promoter

pJ23116

Available since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti CMVtight Blast DEST (w762-1)

Plasmid

#26434

Depositor

Eric Campeau

Type

Empty backbone

Use

Lentiviral; Tet-on advanced destination vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2011

Availability

Academic Institutions and Nonprofits only

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ND4 right TALE–G1397 DddAtox C

Plasmid

#158097

Purpose

expresses the right-side ND4 TALE–DddAtox half in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

COX8A MTS–3xFLAG–ND4 right TALE–G1397 DddA tox-C–1xUGI

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pEF-pIgH-ESCargo(FTV,Crimson)

Plasmid

#140162

Purpose

Expresses a regulatable secretory cargo for mammalian cells

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

piGH-FTVNTT-E2-Crimson-FKBP(LV)(C22V)

Use

Tags

ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a(+)_beta2M (MHC Class I light)

Plasmid

#187357

Purpose

Bacterial expression and refolding of gp100 pMHC

Depositor

Simon Davis

Article

Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

Insert

beta2M (MHC Class I light)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti CMVtight eGFP Blast (w782-1)

Plasmid

#26584

Purpose

2nd generation lentiviral vector

Depositor

Eric Campeau

Insert

Enhanced Green Fluorescent Protein

Use

Lentiviral; Tet-on advanced vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2011

Availability

Academic Institutions and Nonprofits only

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ND4.2-Right DdCBE-G1397-C- T1413I

Plasmid

#179685

Purpose

Expression of base editor in mammalian cells

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

SOD2 MTS_3xHA_ND4.2 Right TALE_2aa_DddA G1397-C (T1413I)_1xUGI_SOD2 3'UTR

Use

Tags

Expression

Mammalian

Mutation

T1413I

Promoter

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti CMVtight eGFP Neo (w784-1)

Plasmid

#26586

Purpose

2nd generation lentiviral vector

Depositor

Eric Campeau

Insert

Enhanced Green Fluorescent Protein

Use

Lentiviral; Tet-on advanced vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pEF-pIgH-ESCargo(FTV,Orange)

Plasmid

#140161

Purpose

Expresses a regulatable secretory cargo for mammalian cells

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

piGH-FTVNTT-E2-Orange-FKBP(LV)(C22V)

Use

Tags

ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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LB1353 (Bcl-2 promoter, no IgH enh)

Plasmid

#15393

Depositor

Linda Boxer

Article

Duan et al Oncogene. 2007 Apr 19. 26(18):2635-41.

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial and Mammalian

Mutation

Bcl-2 with full promoter and no IgH enhancers MHS…

Promoter

Available since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
ND4 right TALE–G1333 DddAtox C

Plasmid

#158095

Purpose

expresses the right-side ND4 TALE–DddAtox half in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

COX8A MTS–3xFLAG–ND4 right TALE–G1333 DddA tox-C–1xUGI

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pTre-Tight-luciferase-GFP (467)

Plasmid

#65491

Purpose

negative control for expression assays

Depositor

Rory Johnson, Roderic Guigo

Insert

EGFP

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

tight TRE promoter

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pLVX-TIGHT-CYCLINF-Tet-OFF

Plasmid

#32977

Depositor

Michele Pagano

Insert

CYCLIN F (CCNF Human)

Use

Lentiviral

Tags

Flag and mCherry

Expression

Mammalian

Mutation

Promoter

P-TIGHT

Available since

Feb. 13, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-ND1 Right-GSVG-UGI

Plasmid

#187413

Purpose

human mitochondrial DNA ND1 target TALE

Depositor

Jin-Soo Kim

Article

Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

Insert

DddA GSVG variant

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pTre-tight-SV40-luciferase (496)

Plasmid

#65489

Purpose

Inducible expression of coding/noncoding genes

Depositor

Rory Johnson, Roderic Guigo

Insert

SV40-luciferase

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

tight Tre promoter

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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Agarabi CHO Light Chain in pJ201

Plasmid

#111566

Purpose

Cloning vector containing CDS for the murine light chain of the model chimeric mouse-human IgG1 expressed by the Agarabi CHO cell line

Depositor

Cyrus Agarabi

Article

Velugula-Yellela et al Biotechnol Prog. 2018 Jan;34(1):262-270.

Insert

Murine light chain

Use

Synthetic Biology; Cloning vector

Tags

Expression

Mutation

Promoter

None

Available since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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pTRE-Tight-FLAG-CYLD-C601A

Plasmid

#60029

Purpose

CYLD (C601A) catalytic dead under control of Tet promoter with N-terminal FLAG tag

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

Insert

Cylindromatosis (CYLD Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Cysteine 601 changed to Alanine

Promoter

TRE

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only