58,075 results

1. pLJM60 FLAG-RAB35 S22N

   Plasmid

   #107106

   Purpose

   Lentiviral vector that codes for FLAG-RAB35 S22N (human) protein. Puromycin resistance for mammalian selection and ampicillin resistance for growth in Stbl3 cells.

   Depositor

   Charles Sawyers

   Article

   Wheeler et al Science. 2015 Oct 9;350(6257):211-7. doi

   Insert

   RAB35 (RAB35 Human)
   

   Use

   Lentiviral

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-C-Myc-DDK-IRES-Puro HEXIM1

   Plasmid

   #200726

   Purpose

   Lentiviral expression of Flag-Tagged human HEXIM1

   Depositor

   Claudio Alarcón

   Insert

   HEXIM1 (HEXIM1 Human)
   

   Use

   Lentiviral

   Tags

   Myc-DDK from backbone plasmid

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJB010

   Plasmid

   #22616

   Depositor

   Lynn Hudson

   Article

   Berndt et al J Biol Chem. 1992 Jul 25. 267(21):14730-

   Insert

   human genomic PLP promoter region (PLP1 Human)
   

   Use

   Phagemid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 24, 2009

   Availability

   Academic Institutions and Nonprofits only

4. TAp63alpha-FLAG

   Plasmid

   #27008

   Depositor

   David Sidransky

   Insert

   TAp63alpha (TP63 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-His6-ERK2-MEK1_R4F_coexpression

   Plasmid

   #39212

   Depositor

   Melanie Cobb

   Article

   Khokhlatchev et al J Biol Chem. 1997 Apr 25;272(17):11057-6

   Inserts

   Mek1 R4F (MAP2K1 Human)
   Erk2 (Mapk1 Rat)
   

   Use

   Tags

   His6 and RBS

   Expression

   Bacterial

   Mutation

   MEK1(R4F) = S218E, S222D and deletion of AA32-51

   Promoter

   T7 and T7 via RBS

   Available since

   July 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti HRE-Luc pGK Hygro

   Plasmid

   #118706

   Purpose

   reporter plasmid HIF-responsive promoter (3xHRE) wt- fused to firefly luciferase

   Depositor

   William Kaelin

   Article

   Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

   Insert

   HRE (HIF-responsive promoter (3xHRE)-luc
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. AASDHPPT

   Plasmid

   #39192

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   AASDHPPT (AASDHPPT Human)
   

   Use

   Tags

   6xHis TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pLJM1 Raptor GFP

   Plasmid

   #112744

   Purpose

   express Raptor-GFP

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

   Insert

   Raptor-GFP (RPTOR Human)
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mutation

   Promoter

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA-FLAG-SF2

   Plasmid

   #99021

   Purpose

   Mammalian expression of SF2

   Depositor

   Honglin Chen

   Article

   Huang et al Nat Commun. 2017 Mar 21;8:14751. doi: 10

   Insert

   SF2 (SRSF1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-hsp27 wt FL

    Plasmid

    #17444

    Depositor

    Andrea Doseff

    Article

    Voss et al J Biol Chem. 2007 Aug 24. 282(34):25088-

    Insert

    small heat shock protein27 (HSPB1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE-3FLAG-RAB11A

    Plasmid

    #176489

    Purpose

    For lentiviral transduction of 3FLAG-RAB11A

    Depositor

    Wade Harper

    Article

    Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

    Insert

    RAB11A (RAB11A Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAIP4_2A4D

    Plasmid

    #234196

    Purpose

    Heterologous protein expression of ubiquitin-conjugating enzyme E2 variant 1 (UEV-1) (CROC-1) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    2A4D (UBE2V1 Human)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pGM238

    Plasmid

    #220178

    Purpose

    Expresses exogenous ribosomal-binding domain mutant NAC complex members (K78E NACA and K43E BTF3)

    Depositor

    Jonathan Weissman

    Article

    Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

    Insert

    NACA and BTF3 (NACA, BTF3 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG (NACA); 6xHis (BTF3)

    Expression

    Mutation

    K78E (NACA) + K43B (BTF3)

    Promoter

    Available since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. SS-Cyclin D1

    Plasmid

    #215113

    Purpose

    Expresses SS-Cyclin D1 in mammalian cells.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Tags

    SS (STREP-STREP)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-sCAG-eGFP-ACAP1

    Plasmid

    #203800

    Purpose

    AAV vector plasmid expressing human ACAP1 fused to eGFP under a short variant of the CAG (sCAG) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis

    Insert

    ACAP1 (ACAP1 Human)
    

    Use

    AAV

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. TFORF0160

    Plasmid

    #141537

    Purpose

    Lentiviral vector for overexpressing the EWSR1 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    EWSR1 (EWSR1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP‐RNASEH2A

    Plasmid

    #108700

    Purpose

    For expression of N-terminally eGFP-tagged human RNASEH2A in mammalian cells

    Depositor

    Martin Reijns, Andrew Jackson

    Article

    Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

    Insert

    RNASEH2A (RNASEH2A Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. phL1A-pcDNA3

    Plasmid

    #12307

    Depositor

    Vance Lemmon

    Article

    Hlavin et al Genomics. 1991 Oct . 11(2):416-23.

    Insert

    L1CAM (L1CAM Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGART-GFP

    Plasmid

    #99106

    Purpose

    Expresses human GART-GFP fusion in mammalian cells

    Depositor

    Stephen Benkovic

    Article

    An et al Science. 2008 Apr 4;320(5872):103-6. doi

    Insert

    GART (GART Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    A65T, L68V, and A72S mutations in GFP gene; kanR/…

    Promoter

    CMV

    Available since

    Dec. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. SpRY-ABE8e(V106W)

    Plasmid

    #198553

    Purpose

    Expresses human codon-optimized SpRY-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpRY-ABE8e(V106W)-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpRY-ABE8e(V106W)
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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