Addgene

We narrowed to 13,587 results for: nts

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pDest17_A1-LCD swap V2

Plasmid

#226539

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD V2

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap V2

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pDest17_A1-LCD swap V3

Plasmid

#226540

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD V3

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap V3

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pDest17_A1-LCD swap X5

Plasmid

#226547

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X5

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X5

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pDest17_A1-LCD swap X7

Plasmid

#226549

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X7

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X7

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pDest17_A1-LCD swap X1

Plasmid

#226543

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X1

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X1

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_A1-LCD swap V5

Plasmid

#226542

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD V5

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap V5

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_A1-LCD swap X4

Plasmid

#226546

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X4

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X4

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_A1-LCD swapX3

Plasmid

#226545

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X3

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X3

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_A1-LCD swap V4

Plasmid

#226541

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD V4

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap V4

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_A1-LCD swap X10

Plasmid

#226552

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X10

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X10

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pDest17_A1-LCD swap X9

Plasmid

#226551

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X9

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X9

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pDest17_A1-LCD swap X8

Plasmid

#226550

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X8

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X8

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pDest17_A1-LCD swap X6

Plasmid

#226548

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X6

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X6

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_A1-LCD swap X2

Plasmid

#226544

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X2

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X2

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pTBL3481 MTK3-mCherry

Plasmid

#226672

Purpose

MTK3 part containing the coding sequence of mCherry.

Depositor

Chang Liu

Article

Loveless et al bioRxiv 2021.11.05.467507

Insert

mCherry

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Removed BbsI sites

Promoter

none

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pTBL2670 MTK3-PEmax

Plasmid

#226663

Purpose

MTK3 part containing the coding sequence of PEmax

Depositor

Chang Liu

Article

Loveless et al bioRxiv 2021.11.05.467507

Insert

PEmax

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

none

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pTBL3241 PB-AtoBpegRNA-mCherry

Plasmid

#226666

Purpose

PiggyBac cargo vector encoding A to B pegRNA marked with mCherry

Depositor

Chang Liu

Article

Loveless et al bioRxiv 2021.11.05.467507

Insert

AtoB pegRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pTBL3401 BtoApegRNA-sfGFP

Plasmid

#226667

Purpose

PiggyBac cargo vector encoding B to A pegRNA marked with sfGFP

Depositor

Chang Liu

Article

Loveless et al bioRxiv 2021.11.05.467507

Insert

BtoA pegRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pBSsgRNA

Plasmid

#224867

Purpose

Yeast genome editing, Insertion of 20 bp oligo into sgRNA gene

Depositor

Katsunori Suzuki

Article

Kiyokawa et al Appl Microbiol Biotechnol. 2024 Jul 12;1

Type

Empty backbone

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-iGlu3slow1

Plasmid

#226501

Purpose

Superslow-decay iGluSnFR3 variant 1 for population imaging

Depositor

Katalin Torok

Article

Tran et al bioRxiv, August 30th, 2024

Insert

iGluSnFR3 D25A

Use

Tags

IgK, Myc tag, and PDGFR Beta TM domain

Expression

Mammalian

Mutation

D25A

Promoter

CMV

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-iGlu3slow2

Plasmid

#226502

Purpose

Superslow-decay iGluSnFR3 variant 2 for population imaging

Depositor

Katalin Torok

Article

Tran et al bioRxiv, August 30th, 2024

Insert

iGluSnFR3 D25R

Use

Tags

IgK, Myc tag, and PDGFR Beta TM domain

Expression

Mammalian

Mutation

D25R

Promoter

CMV

Available since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pBi2FC-N-MPK5-P2A:mCherry

Plasmid

#216136

Purpose

Controls for Bicistronic BiFC

Depositor

Imran Siddiqi

Article

Sivakumar et al Physiol Mol Biol Plants. 2024 Jul;30(7):

Insert

MPK5 (MPK5 Mustard Weed)

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pCPH3-GS3

Plasmid

#204761

Purpose

Expression of Cas9 and human H3

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H3 (H3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCPH3.3-GS3

Plasmid

#204764

Purpose

Expression of Cas9 and human H3.3

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H3.3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pUCIDT-AMP-Fp16SrRNA

Plasmid

#218519

Purpose

pUCIDT-AMP containing 16S rRNA gene of F. prausnitzii for absolute concentration quantification

Depositor

Ami Bhatt

Article

Maghini et al Nat Biotechnol. 2024 Feb;42(2):328-338.

Insert

16S rRNA

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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sgRNA TR KO 2

Plasmid

#207610

Purpose

sgRNA 2 to knockout TR by replacement with a PuroR cassette

Depositor

Jens Schmidt

Article

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

TCAGGCCGCAGGAAGAGGAA

Use

Tags

None

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
sgRNA TR KO 1

Plasmid

#207609

Purpose

sgRNA 1 to knockout TR by replacement with a PuroR cassette

Depositor

Jens Schmidt

Article

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

ACCCTAACTGAGAAGGGCGT

Use

Tags

None

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
CTNNB1 - G752A in pENTR

Plasmid

#216623

Purpose

gateway cloning donor vector containing CTNNB1 ASD mutation

Depositor

Christopher Loewen, Douglas Allan

Insert

CTNNB1 (CTNNB1 Human)

Use

Gateway entry plasmid

Tags

Expression

Mutation

G752A

Promoter

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
CTNNB1 - R673w in pENTR

Plasmid

#216622

Purpose

gateway cloning donor vector containing CTNNB1 ASD mutation

Depositor

Christopher Loewen, Douglas Allan

Insert

CTNNB1 (CTNNB1 Human)

Use

Gateway entry plasmid

Tags

Expression

Mutation

R673W

Promoter

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
CTNNB1 - T551M in pENTR

Plasmid

#216621

Purpose

gateway cloning donor vector containing CTNNB1 ASD mutation

Depositor

Christopher Loewen, Douglas Allan

Insert

CTNNB1 (CTNNB1 Human)

Use

Gateway entry plasmid

Tags

Expression

Mutation

T551M

Promoter

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge