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Plasmid#148949PurposeMammalian Expression of HsGIGYF2_1-718-del280-310DepositorInsertHsGIGYF2_1-718-del280-310 (GIGYF2 Human)
UseTagsExpressionMammalianMutationone silent mutation and one deletion of Q1211 com…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot1iso1_1-1605-IKGRQtoDSYAA-shRNAres_W
Plasmid#147888PurposeMammalian Expression of HsNot1iso1_1-1605-IKGRQtoDSYAA-shRNAresDepositorInsertHsNot1iso1_1-1605-IKGRQtoDSYAA-shRNAres (CNOT1 Human)
UseTagsExpressionMammalianMutation1 silent mutation compared to the sequence of iso…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot1iso1_1-1840-IKGRQtoDSYAA-shRNAres_W
Plasmid#147890PurposeMammalian Expression of HsNot1iso1_1-1840-IKGRQtoDSYAA-shRNAresDepositorInsertHsNot1iso1_1-1840-IKGRQtoDSYAA-shRNAres (CNOT1 Human)
UseTagsExpressionMammalianMutation1 silent mutation compared to the sequence of iso…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0209 CMV-TO-mCherry-SG(s70587)SG-EGFP (FLP-IN)
Plasmid#191174PurposeMammalian expression of EK0285 with CMV-tetO promoter instead of SFFV.DepositorInsertCMV-TO:mCherry:s70587:EGFP
UseTagsExpressionMammalianMutationWTPromoterCMV-TOAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0441 CMV-TO-BFPmut-stop-EGFP.t2 (FLP-IN)
Plasmid#191161PurposeExpression of a trigger sequence from EGFP CDS in 3' UTR context in mammalian cellsDepositorInsertEGFP.t2
UseTagsExpressionMammalianMutationWTPromoterCMV-TOAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0341 SFFV-mCherry-SG(Bdnf.s1L153)SG-EGFP (FLP-IN)
Plasmid#191150PurposeExpression of a 153 bp long sensor for murine Bdnf 3' UTR in mammalian cellsDepositorInsertmCherry:Bdnf.s1L153:EGFP
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
EK0358 SFFV-mCherry-SG(Bdnf.s1L36)SG-EGFP (FLP-IN)
Plasmid#191152PurposeExpression of a 36 bp long sensor for murine Bdnf 3' UTR in mammalian cellsDepositorInsertmCherry:Bdnf.s1L36:EGFP
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-PDGFRA-D842V+R914W_EGFP-PEST_reporter
Plasmid#172599PurposeMammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-D842V+R914W mutant signaling.DepositorInsertmyc-PDGFRA-D842V+R914W (PDGFRA Synthetic, Human)
UseEgfp-pest_reporterTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPDGFRA-D842V+R914WPromoterCMVAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-PDGFRA-K627R+R914W_EGFP-PEST_reporter
Plasmid#172600PurposeMammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-K627R+R914W mutant signaling.DepositorInsertmyc-PDGFRA-K627R+R914W (PDGFRA Synthetic, Human)
UseEgfp-pest_reporterTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPDGFRA-K627R+R914WPromoterCMVAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-Rock1-GBD (840-110 aa) in pEGFPN1
Plasmid#187279PurposeExpress EGFP-Rock1 GTPase-binding domainDepositorInsertRock1 (Rock1 Mouse)
UseTagsEGFPExpressionMammalianMutationGBD (84-110aa)PromoterCMVAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
myr-palm-mEYFP-(L)-mCherry2-(L)-mEGFP-(L)-mApple
Plasmid#182854PurposeHetero-tetrmer expression vector with plasma membrane localization signalDepositorInsertmyr-palm-mEYFP-mCherry2-mEGFP-mApple
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
AiP963-Ai193 (TICL-EGFP-ICF-tdT)
Plasmid#181759Purposetargeting vectorDepositorInsertEGFP and tdTomato
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-LSm-E23K-V5His6_B
Plasmid#145983PurposeInsect Expression of DmTral-LSm-E23KDepositorInsertDmTral-LSm-E23K (tral Fly)
UseTagsExpressionInsectMutationone silent mutation A198C compared to the sequenc…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-LSm-R21E-V5His6_B
Plasmid#145984PurposeInsect Expression of DmTral-LSm-R21EDepositorInsertDmTral-LSm-R21E (tral Fly)
UseTagsExpressionInsectMutationone silent mutation A198C compared to the sequenc…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-LSmTex-del46-49-V5His6_B
Plasmid#145985PurposeInsect Expression of DmTral-LSmTex-del46-49DepositorInsertDmTral-LSmTex-del46-49 (tral Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-Dm4E-T_1-510-V5His6_D
Plasmid#146149PurposeInsect Expression of Dm4E-T_1-510DepositorInsertDm4E-T_1-510 (4E-T Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-LSm-F44A-V5His6_C
Plasmid#146047PurposeInsect Expression of DmTral-LSm-F44ADepositorInsertDmTral-LSm-F44A (tral Fly)
UseTagsExpressionInsectMutationone silent mutation A198C compared to the sequenc…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-LSm-R21EE23KR42EF44A-V5His6_C
Plasmid#146048PurposeInsect Expression of DmTral-LSm-R21EE23KR42EF44ADepositorInsertDmTral-LSm-R21EE23KR42EF44A (tral Fly)
UseTagsExpressionInsectMutationone silent mutation A198C compared to the sequenc…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-LSm-R21EE23K-V5His6_C
Plasmid#146050PurposeInsect Expression of DmTral-LSm-R21EE23KDepositorInsertDmTral-LSm-R21EE23K (tral Fly)
UseTagsExpressionInsectMutationone silent mutation A198C compared to the sequenc…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-LSm-R21EF44A-V5His6_C
Plasmid#146051PurposeInsect Expression of DmTral-LSm-R21EF44ADepositorInsertDmTral-LSm-R21EF44A (tral Fly)
UseTagsExpressionInsectMutationone silent mutation A198C compared to the sequenc…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-LSm-R21ER42EF44A-V5His6_C
Plasmid#146052PurposeInsect Expression of DmTral-LSm-R21ER42EF44ADepositorInsertDmTral-LSm-R21ER42EF44A (tral Fly)
UseTagsExpressionInsectMutationone silent mutation A198C compared to the sequenc…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-LSm-R21ES13AI15A-V5His6_C
Plasmid#146053PurposeInsect Expression of DmTral-LSm-R21ES13AI15ADepositorInsertDmTral-LSm-R21ES13AI15A (tral Fly)
UseTagsExpressionInsectMutationone silent mutation A198C compared to the sequenc…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-LSm-R42EF44A-V5His6_C
Plasmid#146054PurposeInsect Expression of DmTral-LSm-R42EF44ADepositorInsertDmTral-LSm-R42EF44A (tral Fly)
UseTagsExpressionInsectMutationone silent mutation A198C compared to the sequenc…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-LSm-S13AI15A-V5His6_C
Plasmid#146055PurposeInsect Expression of DmTral-LSm-S13AI15ADepositorInsertDmTral-LSm-S13AI15A (tral Fly)
UseTagsExpressionInsectMutationone silent mutation A198C compared to the sequenc…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-LSm-del46-60-V5His6_C
Plasmid#146046PurposeInsect Expression of DmTral-LSm-del46-60DepositorInsertDmTral-LSm-del46-60 (tral Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmSmaug-del584-762-V5His6_C
Plasmid#146006PurposeInsect Expression of DmSmaug-del584-762DepositorInsertDmSmaug-del584-762 (smg Fly)
UseTagsExpressionInsectMutation7 silent mutations and a deletion of the AA 961-9…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET29b-AviTag-mEGFP-dspB(E184Q)-6xHis
Plasmid#176573PurposePlasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
UseTagsAvitag, Hexahistidine tag, and mEGFPExpressionBacterialMutationE184QPromoterT7Available sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-SPN_ARR Q37A/R38A/N52R (Shank3)
Plasmid#175256PurposeSPN-ARR fragment with structure opening N52R and actin binding site Q37A/R38A mutations in EGFP backbone for mammalian expressionDepositorInsertSPN-ARR fragment with Q37A/R38A/N52R mutations from SHANK3
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-LSm-R21EE23KS13AI15A-V5His6_C
Plasmid#146049PurposeInsect Expression of DmTral-LSmR21EE23KS13AI15ADepositorInsertDmTral-LSmR21EE23KS13AI15A (tral Fly)
UseTagsExpressionInsectMutationS13A, I15A, R21E, E23K, one silent mutation A198C…PromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
C512-E01: attB5r-meGFP-nostop-attB1r
Plasmid#162940PurposeGateway attB5r/attB1r entry clone for generation of N-terminal eGFP fusion proteinsDepositorInsertmonomeric enhanced Green fluorescent protein
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-mEGFP(A206K)-paCaMKII(I539E)-ShadowG
Plasmid#167478PurposeExpresses paCaMKII in mammalian cells to measure structural change of paCaMKII.DepositorInsertmEGFP(A206K)-paCaMKII(I539E)-ShadowG
UseTagsShadowG and mEGFP(A206K)ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-mEGFP(A206K)-paCaMKII(C450A)-ShadowG
Plasmid#167479PurposeExpresses paCaMKII in mammalian cells to measure structural change of paCaMKII.DepositorInsertmEGFP(A206K)-paCaMKII(C450A)-ShadowG
UseTagsShadowG and mEGFP(A206K)ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Mm_Plk5(300-595) PBD domain
Plasmid#136316PurposeMammalian expression of mouse Plk5-D300 (PBD domain 300-595) with N-terminal EGFPDepositorInsertPLK5 (Plk5 Mouse)
UseTagsExpressionMammalianMutationfragment 300-595PromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP(1-233)-mApple(2-236)-HRas(2-189)
Plasmid#133855PurposeMammalian expression of HRas N-terminally tagged with EGFP and mAppleDepositorInsertHRas (HRAS Human)
UseTagsEGFP and mAppleExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
phage - ubc - nls - 2xmcp - egfp - cMycBirA*
Plasmid#131133PurposeBirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.DepositorInsertNLS-HA-2xMCP-EGFP-cMycBirA*
UseLentiviralTagsNLS, HA, cMyc (N-terminal of BirA*)ExpressionMammalianMutationBirA* = BIrA >R118GPromoterhuman ubiquitin C (UBC)Available sinceAug. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
JN511: pMVP (L3-L2) pA; EF1a::eGFP-P2A-TETa-pA
Plasmid#121803PurposepMVP L3-L2 entry plasmid, contains polyA + EF1a-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds EF1a-driven GFP-P2A-TETa downstream of gene of interest.DepositorInsertpolyA + EF1a::eGFP-P2A-TETa-polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IG806: pMVP (L3-L2) pA; RIP::eGFP-P2A-TETa-pA
Plasmid#121807PurposepMVP L3-L2 entry plasmid, contains polyA + RIP-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds RIP-driven GFP-P2A-TETa downstream of gene of interest.DepositorInsertpolyA + RIP::eGFP-P2A-TETa-polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IX702: pMVP (L3-L2) OLLAS-IRES-eGFP + polyA
Plasmid#121748PurposepMVP L3-L2 entry plasmid, contains OLLAS epitope tag + IRES2-eGFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion and IRES expression of eGFP reporterDepositorInsertOLLAS epitope tag-IRES2-eGFP + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1088 - pAAV TH gRNA A+B pair EF1a EGFP
Plasmid#113155PurposeAn AAV vector that expresses guide RNAs targeting rat TH and expresses EGFP reporterDepositorInsertTwo gRNAs for rat TH
UseAAV and CRISPRTagsExpressionMammalianMutationPromotermU6 and hU6Available sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1388 - pAAV MANF gRNA A+B EF1a EGFP
Plasmid#113157PurposeAn AAV vector that expresses guide RNAs targeting rat MANF and expresses EGFP reporterDepositorInsertTwo gRNAs for rat MANF
UseAAV and CRISPRTagsExpressionMammalianMutationPromotermU6 and hU6Available sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1 Dynamin1 T78R L84R T92R V118R
Plasmid#112106PurposeMammalian expression plasmid of GFP-tagged Dynamin protein.DepositorInsertDynamin1 (DNM1 Human)
UseTagsEGFPExpressionMammalianMutationT78R L84R T92R V118RPromoterCMVAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p15A-Pl-lacO1-deGFP-T500 (123p)
Plasmid#70199PurposePromoter panelDepositorInsertdeGFP
UseTagsExpressionMutationPromoterAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p15A-J23113-deGFP-T500 (124p)
Plasmid#70200PurposePromoter panelDepositorInsertdeGFP
UseTagsExpressionMutationPromoterAvailable sinceFeb. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p15A-pNull-deGFP-T500 (133p)
Plasmid#70209PurposePromoter panelDepositorInsertdeGFP
UseTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p15A-J23102-deGFP-T500 (132p)
Plasmid#70208PurposePromoter panelDepositorInsertdeGFP
UseTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p15A-J23100-deGFP-T500 (130p)
Plasmid#70206PurposePromoter panelDepositorInsertdeGFP
UseTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p15A-J23151-deGFP-T500 (129p)
Plasmid#70205PurposePromoter panelDepositorInsertdeGFP
UseTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p15A-J23106-deGFP-T500 (128p)
Plasmid#70204PurposePromoter panelDepositorInsertdeGFP
UseTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p15A-J23150-deGFP-T500 (127p)
Plasmid#70203PurposePromoter panelDepositorInsertdeGFP
UseTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p15A-J23116-deGFP-T500 (126p)
Plasmid#70202PurposePromoter panelDepositorInsertdeGFP
UseTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only